Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6KYK

Crystal structure of Shank3 NTD-ANK mutant in complex with Rap1

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005811	cellular_component	lipid droplet
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005911	cellular_component	cell-cell junction
C	0007165	biological_process	signal transduction
C	0007264	biological_process	small GTPase-mediated signal transduction
C	0008283	biological_process	cell population proliferation
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0017156	biological_process	calcium-ion regulated exocytosis
C	0019003	molecular_function	GDP binding
C	0030033	biological_process	microvillus assembly
C	0032486	biological_process	Rap protein signal transduction
C	0033625	biological_process	positive regulation of integrin activation
C	0035577	cellular_component	azurophil granule membrane
C	0044877	molecular_function	protein-containing complex binding
C	0045121	cellular_component	membrane raft
C	0045955	biological_process	negative regulation of calcium ion-dependent exocytosis
C	0051649	biological_process	establishment of localization in cell
C	0061028	biological_process	establishment of endothelial barrier
C	0070062	cellular_component	extracellular exosome
C	0070161	cellular_component	anchoring junction
C	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
C	0071320	biological_process	cellular response to cAMP
C	0098978	cellular_component	glutamatergic synapse
C	0099010	biological_process	modification of postsynaptic structure
C	1901888	biological_process	regulation of cell junction assembly
C	2000114	biological_process	regulation of establishment of cell polarity
C	2000301	biological_process	negative regulation of synaptic vesicle exocytosis
D	0003924	molecular_function	GTPase activity
D	0003925	molecular_function	G protein activity
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005811	cellular_component	lipid droplet
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005911	cellular_component	cell-cell junction
D	0007165	biological_process	signal transduction
D	0007264	biological_process	small GTPase-mediated signal transduction
D	0008283	biological_process	cell population proliferation
D	0016020	cellular_component	membrane
D	0016787	molecular_function	hydrolase activity
D	0017156	biological_process	calcium-ion regulated exocytosis
D	0019003	molecular_function	GDP binding
D	0030033	biological_process	microvillus assembly
D	0032486	biological_process	Rap protein signal transduction
D	0033625	biological_process	positive regulation of integrin activation
D	0035577	cellular_component	azurophil granule membrane
D	0044877	molecular_function	protein-containing complex binding
D	0045121	cellular_component	membrane raft
D	0045955	biological_process	negative regulation of calcium ion-dependent exocytosis
D	0051649	biological_process	establishment of localization in cell
D	0061028	biological_process	establishment of endothelial barrier
D	0070062	cellular_component	extracellular exosome
D	0070161	cellular_component	anchoring junction
D	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
D	0071320	biological_process	cellular response to cAMP
D	0098978	cellular_component	glutamatergic synapse
D	0099010	biological_process	modification of postsynaptic structure
D	1901888	biological_process	regulation of cell junction assembly
D	2000114	biological_process	regulation of establishment of cell polarity
D	2000301	biological_process	negative regulation of synaptic vesicle exocytosis
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005811	cellular_component	lipid droplet
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005911	cellular_component	cell-cell junction
E	0007165	biological_process	signal transduction
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0008283	biological_process	cell population proliferation
E	0016020	cellular_component	membrane
E	0016787	molecular_function	hydrolase activity
E	0017156	biological_process	calcium-ion regulated exocytosis
E	0019003	molecular_function	GDP binding
E	0030033	biological_process	microvillus assembly
E	0032486	biological_process	Rap protein signal transduction
E	0033625	biological_process	positive regulation of integrin activation
E	0035577	cellular_component	azurophil granule membrane
E	0044877	molecular_function	protein-containing complex binding
E	0045121	cellular_component	membrane raft
E	0045955	biological_process	negative regulation of calcium ion-dependent exocytosis
E	0051649	biological_process	establishment of localization in cell
E	0061028	biological_process	establishment of endothelial barrier
E	0070062	cellular_component	extracellular exosome
E	0070161	cellular_component	anchoring junction
E	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
E	0071320	biological_process	cellular response to cAMP
E	0098978	cellular_component	glutamatergic synapse
E	0099010	biological_process	modification of postsynaptic structure
E	1901888	biological_process	regulation of cell junction assembly
E	2000114	biological_process	regulation of establishment of cell polarity
E	2000301	biological_process	negative regulation of synaptic vesicle exocytosis
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005737	cellular_component	cytoplasm
F	0005811	cellular_component	lipid droplet
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005911	cellular_component	cell-cell junction
F	0007165	biological_process	signal transduction
F	0007264	biological_process	small GTPase-mediated signal transduction
F	0008283	biological_process	cell population proliferation
F	0016020	cellular_component	membrane
F	0016787	molecular_function	hydrolase activity
F	0017156	biological_process	calcium-ion regulated exocytosis
F	0019003	molecular_function	GDP binding
F	0030033	biological_process	microvillus assembly
F	0032486	biological_process	Rap protein signal transduction
F	0033625	biological_process	positive regulation of integrin activation
F	0035577	cellular_component	azurophil granule membrane
F	0044877	molecular_function	protein-containing complex binding
F	0045121	cellular_component	membrane raft
F	0045955	biological_process	negative regulation of calcium ion-dependent exocytosis
F	0051649	biological_process	establishment of localization in cell
F	0061028	biological_process	establishment of endothelial barrier
F	0070062	cellular_component	extracellular exosome
F	0070161	cellular_component	anchoring junction
F	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
F	0071320	biological_process	cellular response to cAMP
F	0098978	cellular_component	glutamatergic synapse
F	0099010	biological_process	modification of postsynaptic structure
F	1901888	biological_process	regulation of cell junction assembly
F	2000114	biological_process	regulation of establishment of cell polarity
F	2000301	biological_process	negative regulation of synaptic vesicle exocytosis

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	binding site for residue GNP C 201

Chain	Residue
C	GLY12
C	GLU30
C	TYR32
C	PRO34
C	THR35
C	ALA59
C	GLY60
C	ASN116
C	LYS117
C	ASP119
C	SER147
C	GLY13
C	ALA148
C	LYS149
C	MG202
C	HOH301
C	HOH303
C	VAL14
C	GLY15
C	LYS16
C	SER17
C	ALA18
C	PHE28
C	VAL29

site_id	AC2
Number of Residues	6
Details	binding site for residue MG C 202

Chain	Residue
C	SER17
C	THR35
C	ASP57
C	GNP201
C	HOH301
C	HOH303

site_id	AC3
Number of Residues	24
Details	binding site for residue GNP D 201

Chain	Residue
D	GLY12
D	GLY13
D	VAL14
D	GLY15
D	LYS16
D	SER17
D	ALA18
D	PHE28
D	VAL29
D	GLU30
D	TYR32
D	PRO34
D	THR35
D	ALA59
D	GLY60
D	ASN116
D	LYS117
D	ASP119
D	LEU120
D	SER147
D	ALA148
D	MG202
D	HOH303
D	HOH305

site_id	AC4
Number of Residues	5
Details	binding site for residue MG D 202

Chain	Residue
D	SER17
D	THR35
D	GNP201
D	HOH303
D	HOH305

site_id	AC5
Number of Residues	24
Details	binding site for residue GNP E 201

Chain	Residue
E	GLY13
E	VAL14
E	GLY15
E	LYS16
E	SER17
E	ALA18
E	PHE28
E	VAL29
E	GLU30
E	LYS31
E	TYR32
E	PRO34
E	THR35
E	ALA59
E	GLY60
E	ASN116
E	LYS117
E	ASP119
E	SER147
E	ALA148
E	LYS149
E	MG202
E	HOH302
E	HOH303

site_id	AC6
Number of Residues	6
Details	binding site for residue MG E 202

Chain	Residue
E	SER17
E	THR35
E	ASP57
E	GNP201
E	HOH302
E	HOH303

site_id	AC7
Number of Residues	23
Details	binding site for residue GNP F 201

Chain	Residue
F	THR35
F	ALA59
F	GLY60
F	ASN116
F	LYS117
F	ASP119
F	LEU120
F	SER147
F	ALA148
F	LYS149
F	MG202
F	HOH301
F	HOH303
F	GLY13
F	GLY15
F	LYS16
F	SER17
F	ALA18
F	PHE28
F	VAL29
F	GLU30
F	TYR32
F	PRO34

site_id	AC8
Number of Residues	5
Details	binding site for residue MG F 202

Chain	Residue
F	SER17
F	THR35
F	GNP201
F	HOH301
F	HOH303

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:18309292, ECO:0000269\|PubMed:22577140

Chain	Residue	Details
C	GLY10
C	ASN116
C	SER147
D	GLY10
D	ASP57
D	ASN116
D	SER147
E	GLY10
E	ASP57
E	ASN116
E	SER147
F	GLY10
F	ASP57
F	ASN116
F	SER147
C	ASP57

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: ADP-ribosylserine; by botulinum toxin => ECO:0000305\|PubMed:3141412

Chain	Residue	Details
C	SER39
D	SER39
E	SER39
F	SER39

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)