Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6KPJ

298 K cryoEM structure of Sso-KARI in complex with Mg2+, NADH and CPD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004455	molecular_function	ketol-acid reductoisomerase activity
A	0008652	biological_process	amino acid biosynthetic process
A	0009082	biological_process	branched-chain amino acid biosynthetic process
A	0009097	biological_process	isoleucine biosynthetic process
A	0009099	biological_process	L-valine biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
B	0004455	molecular_function	ketol-acid reductoisomerase activity
B	0008652	biological_process	amino acid biosynthetic process
B	0009082	biological_process	branched-chain amino acid biosynthetic process
B	0009097	biological_process	isoleucine biosynthetic process
B	0009099	biological_process	L-valine biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
C	0004455	molecular_function	ketol-acid reductoisomerase activity
C	0008652	biological_process	amino acid biosynthetic process
C	0009082	biological_process	branched-chain amino acid biosynthetic process
C	0009097	biological_process	isoleucine biosynthetic process
C	0009099	biological_process	L-valine biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
D	0004455	molecular_function	ketol-acid reductoisomerase activity
D	0008652	biological_process	amino acid biosynthetic process
D	0009082	biological_process	branched-chain amino acid biosynthetic process
D	0009097	biological_process	isoleucine biosynthetic process
D	0009099	biological_process	L-valine biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
E	0004455	molecular_function	ketol-acid reductoisomerase activity
E	0008652	biological_process	amino acid biosynthetic process
E	0009082	biological_process	branched-chain amino acid biosynthetic process
E	0009097	biological_process	isoleucine biosynthetic process
E	0009099	biological_process	L-valine biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
F	0004455	molecular_function	ketol-acid reductoisomerase activity
F	0008652	biological_process	amino acid biosynthetic process
F	0009082	biological_process	branched-chain amino acid biosynthetic process
F	0009097	biological_process	isoleucine biosynthetic process
F	0009099	biological_process	L-valine biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
G	0004455	molecular_function	ketol-acid reductoisomerase activity
G	0008652	biological_process	amino acid biosynthetic process
G	0009082	biological_process	branched-chain amino acid biosynthetic process
G	0009097	biological_process	isoleucine biosynthetic process
G	0009099	biological_process	L-valine biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
H	0004455	molecular_function	ketol-acid reductoisomerase activity
H	0008652	biological_process	amino acid biosynthetic process
H	0009082	biological_process	branched-chain amino acid biosynthetic process
H	0009097	biological_process	isoleucine biosynthetic process
H	0009099	biological_process	L-valine biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
I	0004455	molecular_function	ketol-acid reductoisomerase activity
I	0008652	biological_process	amino acid biosynthetic process
I	0009082	biological_process	branched-chain amino acid biosynthetic process
I	0009097	biological_process	isoleucine biosynthetic process
I	0009099	biological_process	L-valine biosynthetic process
I	0016491	molecular_function	oxidoreductase activity
J	0004455	molecular_function	ketol-acid reductoisomerase activity
J	0008652	biological_process	amino acid biosynthetic process
J	0009082	biological_process	branched-chain amino acid biosynthetic process
J	0009097	biological_process	isoleucine biosynthetic process
J	0009099	biological_process	L-valine biosynthetic process
J	0016491	molecular_function	oxidoreductase activity
K	0004455	molecular_function	ketol-acid reductoisomerase activity
K	0008652	biological_process	amino acid biosynthetic process
K	0009082	biological_process	branched-chain amino acid biosynthetic process
K	0009097	biological_process	isoleucine biosynthetic process
K	0009099	biological_process	L-valine biosynthetic process
K	0016491	molecular_function	oxidoreductase activity
L	0004455	molecular_function	ketol-acid reductoisomerase activity
L	0008652	biological_process	amino acid biosynthetic process
L	0009082	biological_process	branched-chain amino acid biosynthetic process
L	0009097	biological_process	isoleucine biosynthetic process
L	0009099	biological_process	L-valine biosynthetic process
L	0016491	molecular_function	oxidoreductase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MG A 401

Chain	Residue
A	GLU227
A	GLU233
A	MG404
B	ASP191
B	9TY404

site_id	AC2
Number of Residues	13
Details	binding site for residue NAI A 402

Chain	Residue
A	GLN26
A	ASN45
A	TYR50
A	ILE78
A	ASP80
A	ALA106
A	PRO129
A	VAL132
A	9TY403
A	GLY22
A	TYR23
A	GLY24
A	ASN25

site_id	AC3
Number of Residues	4
Details	binding site for residue 9TY A 403

Chain	Residue
A	ASP191
A	GLU195
A	NAI402
B	MG401

site_id	AC4
Number of Residues	5
Details	binding site for residue MG A 404

Chain	Residue
A	GLU227
A	GLU233
A	MG401
B	ASP191
B	9TY404

site_id	AC5
Number of Residues	4
Details	binding site for residue MG B 401

Chain	Residue
A	ASP191
A	9TY403
B	GLU227
B	GLU233

site_id	AC6
Number of Residues	3
Details	binding site for residue MG B 402

Chain	Residue
B	ASP191
B	GLU195
B	9TY404

site_id	AC7
Number of Residues	11
Details	binding site for residue NAI B 403

Chain	Residue
B	GLY22
B	TYR23
B	ASN25
B	GLN26
B	ASN45
B	TYR50
B	ILE78
B	PRO79
B	ASP80
B	VAL132
B	9TY404

site_id	AC8
Number of Residues	6
Details	binding site for residue 9TY B 404

Chain	Residue
A	MG401
A	MG404
B	ASP191
B	GLU195
B	MG402
B	NAI403

site_id	AC9
Number of Residues	5
Details	binding site for residue MG C 401

Chain	Residue
C	GLU227
C	GLU233
C	MG404
D	ASP191
D	9TY404

site_id	AD1
Number of Residues	12
Details	binding site for residue NAI C 402

Chain	Residue
C	GLY22
C	TYR23
C	GLY24
C	ASN25
C	GLN26
C	ASN45
C	TYR50
C	ILE78
C	PRO79
C	ASP80
C	SER107
C	VAL132

site_id	AD2
Number of Residues	4
Details	binding site for residue 9TY C 403

Chain	Residue
C	ASP191
C	GLU195
D	SER254
D	MG401

site_id	AD3
Number of Residues	4
Details	binding site for residue MG C 404

Chain	Residue
C	GLU227
C	MG401
D	ASP191
D	9TY404

site_id	AD4
Number of Residues	4
Details	binding site for residue MG D 401

Chain	Residue
C	ASP191
C	9TY403
D	GLU227
D	GLU233

site_id	AD5
Number of Residues	3
Details	binding site for residue MG D 402

Chain	Residue
D	ASP191
D	GLU195
D	9TY404

site_id	AD6
Number of Residues	14
Details	binding site for residue NAI D 403

Chain	Residue
D	GLY22
D	TYR23
D	GLY24
D	ASN25
D	GLN26
D	ASN45
D	TYR50
D	ILE78
D	PRO79
D	ASP80
D	ALA106
D	PRO129
D	VAL132
D	9TY404

site_id	AD7
Number of Residues	6
Details	binding site for residue 9TY D 404

Chain	Residue
D	ASP191
D	GLU195
D	MG402
D	NAI403
C	MG401
C	MG404

site_id	AD8
Number of Residues	6
Details	binding site for residue MG E 401

Chain	Residue
E	GLU227
E	GLU233
E	MG404
F	ARG130
F	ASP191
F	9TY404

site_id	AD9
Number of Residues	13
Details	binding site for residue NAI E 402

Chain	Residue
E	GLY22
E	TYR23
E	GLY24
E	ASN25
E	GLN26
E	ASN45
E	TYR50
E	ILE78
E	ASP80
E	ALA106
E	PRO129
E	VAL132
E	9TY403

site_id	AE1
Number of Residues	4
Details	binding site for residue 9TY E 403

Chain	Residue
E	ASP191
E	GLU195
E	NAI402
F	MG401

site_id	AE2
Number of Residues	5
Details	binding site for residue MG E 404

Chain	Residue
E	GLU227
E	GLU233
E	MG401
F	ASP191
F	9TY404

site_id	AE3
Number of Residues	4
Details	binding site for residue MG F 401

Chain	Residue
E	ASP191
E	9TY403
F	GLU227
F	GLU233

site_id	AE4
Number of Residues	3
Details	binding site for residue MG F 402

Chain	Residue
F	ASP191
F	GLU195
F	9TY404

site_id	AE5
Number of Residues	11
Details	binding site for residue NAI F 403

Chain	Residue
F	GLY22
F	TYR23
F	ASN25
F	GLN26
F	ASN45
F	TYR50
F	ILE78
F	PRO79
F	ASP80
F	SER107
F	VAL132

site_id	AE6
Number of Residues	6
Details	binding site for residue 9TY F 404

Chain	Residue
E	SER254
E	MG401
E	MG404
F	ASP191
F	GLU195
F	MG402

site_id	AE7
Number of Residues	5
Details	binding site for residue MG G 401

Chain	Residue
G	GLU227
G	GLU233
G	MG404
H	ASP191
H	9TY404

site_id	AE8
Number of Residues	12
Details	binding site for residue NAI G 402

Chain	Residue
G	GLY22
G	TYR23
G	GLY24
G	ASN25
G	GLN26
G	ASN45
G	TYR50
G	ILE78
G	ASP80
G	ALA106
G	VAL132
G	9TY403

site_id	AE9
Number of Residues	4
Details	binding site for residue 9TY G 403

Chain	Residue
G	ASP191
G	GLU195
G	NAI402
H	MG401

site_id	AF1
Number of Residues	5
Details	binding site for residue MG G 404

Chain	Residue
G	GLU227
G	GLU233
G	MG401
H	ASP191
H	9TY404

site_id	AF2
Number of Residues	4
Details	binding site for residue MG H 401

Chain	Residue
G	ASP191
G	9TY403
H	GLU227
H	GLU233

site_id	AF3
Number of Residues	3
Details	binding site for residue MG H 402

Chain	Residue
H	ASP191
H	GLU195
H	9TY404

site_id	AF4
Number of Residues	11
Details	binding site for residue NAI H 403

Chain	Residue
H	GLY22
H	TYR23
H	ASN25
H	GLN26
H	ASN45
H	TYR50
H	ILE78
H	ASP80
H	ALA106
H	VAL132
H	9TY404

site_id	AF5
Number of Residues	6
Details	binding site for residue 9TY H 404

Chain	Residue
G	MG401
G	MG404
H	ASP191
H	GLU195
H	MG402
H	NAI403

site_id	AF6
Number of Residues	5
Details	binding site for residue MG I 401

Chain	Residue
I	GLU227
I	GLU233
I	MG404
J	ASP191
J	9TY404

site_id	AF7
Number of Residues	13
Details	binding site for residue NAI I 402

Chain	Residue
I	GLY22
I	TYR23
I	GLY24
I	ASN25
I	GLN26
I	ASN45
I	TYR50
I	ILE78
I	ASP80
I	ALA106
I	PRO129
I	VAL132
I	9TY403

site_id	AF8
Number of Residues	4
Details	binding site for residue 9TY I 403

Chain	Residue
I	ASP191
I	GLU195
I	NAI402
J	MG401

site_id	AF9
Number of Residues	5
Details	binding site for residue MG I 404

Chain	Residue
I	GLU227
I	GLU233
I	MG401
J	ASP191
J	9TY404

site_id	AG1
Number of Residues	4
Details	binding site for residue MG J 401

Chain	Residue
I	ASP191
I	9TY403
J	GLU227
J	GLU233

site_id	AG2
Number of Residues	3
Details	binding site for residue MG J 402

Chain	Residue
J	ASP191
J	GLU195
J	9TY404

site_id	AG3
Number of Residues	12
Details	binding site for residue NAI J 403

Chain	Residue
J	GLY22
J	TYR23
J	GLY24
J	ASN25
J	GLN26
J	ASN45
J	TYR50
J	ILE78
J	ASP80
J	ALA106
J	VAL132
J	9TY404

site_id	AG4
Number of Residues	7
Details	binding site for residue 9TY J 404

Chain	Residue
I	SER254
I	MG401
I	MG404
J	ASP191
J	GLU195
J	MG402
J	NAI403

site_id	AG5
Number of Residues	6
Details	binding site for residue MG K 401

Chain	Residue
K	GLU227
K	GLU233
K	MG404
L	ARG130
L	ASP191
L	9TY404

site_id	AG6
Number of Residues	13
Details	binding site for residue NAI K 402

Chain	Residue
K	GLY22
K	TYR23
K	GLY24
K	ASN25
K	GLN26
K	ASN45
K	TYR50
K	ILE78
K	ASP80
K	ALA106
K	PRO129
K	VAL132
K	9TY403

site_id	AG7
Number of Residues	4
Details	binding site for residue 9TY K 403

Chain	Residue
K	ASP191
K	GLU195
K	NAI402
L	MG401

site_id	AG8
Number of Residues	5
Details	binding site for residue MG K 404

Chain	Residue
K	GLU227
K	GLU233
K	MG401
L	ASP191
L	9TY404

site_id	AG9
Number of Residues	5
Details	binding site for residue MG L 401

Chain	Residue
K	ARG130
K	ASP191
K	9TY403
L	GLU227
L	GLU233

site_id	AH1
Number of Residues	3
Details	binding site for residue MG L 402

Chain	Residue
L	ASP191
L	GLU195
L	9TY404

site_id	AH2
Number of Residues	13
Details	binding site for residue NAI L 403

Chain	Residue
L	GLY22
L	TYR23
L	GLY24
L	ASN25
L	GLN26
L	ASN45
L	TYR50
L	ILE78
L	ASP80
L	ALA106
L	PRO129
L	VAL132
L	9TY404

site_id	AH3
Number of Residues	7
Details	binding site for residue 9TY L 404

Chain	Residue
K	SER254
K	MG401
K	MG404
L	ASP191
L	GLU195
L	MG402
L	NAI403

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1752
Details	Domain: {"description":"KARI C-terminal knotted","evidences":[{"source":"PROSITE-ProRule","id":"PRU01198","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)