6JML
Re-refined structure of R-state L-lactate dehydrogenase fromLactobacillus casei
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0006090 | biological_process | pyruvate metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0006090 | biological_process | pyruvate metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0006090 | biological_process | pyruvate metabolic process |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0006090 | biological_process | pyruvate metabolic process |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006089 | biological_process | lactate metabolic process |
| E | 0006090 | biological_process | pyruvate metabolic process |
| E | 0006096 | biological_process | glycolytic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| E | 0019752 | biological_process | carboxylic acid metabolic process |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006089 | biological_process | lactate metabolic process |
| F | 0006090 | biological_process | pyruvate metabolic process |
| F | 0006096 | biological_process | glycolytic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| F | 0019752 | biological_process | carboxylic acid metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 A 401 |
| Chain | Residue |
| A | LEU153 |
| A | ARG157 |
| A | HIS181 |
| A | ALA225 |
| A | THR235 |
| A | HOH502 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | binding site for residue SO4 A 402 |
| Chain | Residue |
| A | HOH505 |
| A | HOH520 |
| B | SER172 |
| B | HIS174 |
| B | GLY195 |
| B | HOH509 |
| B | HOH530 |
| A | ARG159 |
| A | HIS174 |
| A | TYR176 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 B 401 |
| Chain | Residue |
| B | LEU153 |
| B | ARG157 |
| B | HIS181 |
| B | ALA225 |
| B | THR235 |
| B | HOH523 |
| B | HOH527 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | binding site for residue SO4 B 402 |
| Chain | Residue |
| A | SER172 |
| A | HIS174 |
| A | GLY195 |
| A | HOH539 |
| B | ARG159 |
| B | HIS174 |
| B | TYR176 |
| B | HOH520 |
| B | HOH536 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 C 401 |
| Chain | Residue |
| C | LEU153 |
| C | ARG157 |
| C | HIS181 |
| C | ALA225 |
| C | THR235 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue SO4 C 402 |
| Chain | Residue |
| C | ARG159 |
| C | HIS174 |
| C | TYR176 |
| C | HOH503 |
| C | HOH509 |
| C | HOH524 |
| C | HOH530 |
| F | SER172 |
| F | HIS174 |
| F | GLY195 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 D 401 |
| Chain | Residue |
| D | LEU153 |
| D | ARG157 |
| D | HIS181 |
| D | ALA225 |
| D | THR235 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 D 402 |
| Chain | Residue |
| D | ARG159 |
| D | HIS174 |
| D | TYR176 |
| D | HOH501 |
| E | HIS174 |
| E | HOH509 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 E 401 |
| Chain | Residue |
| E | ASN126 |
| E | LEU153 |
| E | ARG157 |
| E | HIS181 |
| E | ALA225 |
| E | THR235 |
| site_id | AD1 |
| Number of Residues | 10 |
| Details | binding site for residue SO4 E 402 |
| Chain | Residue |
| D | SER172 |
| D | HIS174 |
| D | GLY195 |
| D | HOH503 |
| E | ARG159 |
| E | HIS174 |
| E | TYR176 |
| E | HOH504 |
| E | HOH507 |
| E | HOH508 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 F 401 |
| Chain | Residue |
| F | LEU153 |
| F | ARG157 |
| F | HIS181 |
| F | ALA225 |
| F | THR235 |
| F | HOH501 |
| F | HOH504 |
| site_id | AD3 |
| Number of Residues | 9 |
| Details | binding site for residue SO4 F 402 |
| Chain | Residue |
| C | HIS174 |
| C | GLY195 |
| C | HOH502 |
| F | ARG159 |
| F | HIS174 |
| F | TYR176 |
| F | HOH506 |
| F | HOH509 |
| F | HOH518 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. MGEHGDT |
| Chain | Residue | Details |
| A | MET178-THR184 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305|Ref.6, ECO:0000305|Ref.8, ECO:0007744|PDB:1LLC, ECO:0007744|PDB:6J9T, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | HIS181 | |
| B | HIS181 | |
| C | HIS181 | |
| D | HIS181 | |
| E | HIS181 | |
| F | HIS181 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 60 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488 |
| Chain | Residue | Details |
| A | VAL20 | |
| A | HIS174 | |
| B | VAL20 | |
| B | ASP41 | |
| B | LYS46 | |
| B | TYR71 | |
| B | GLY85 | |
| B | GLN88 | |
| B | SER107 | |
| B | ALA124 | |
| B | SER149 | |
| A | ASP41 | |
| B | HIS174 | |
| C | VAL20 | |
| C | ASP41 | |
| C | LYS46 | |
| C | TYR71 | |
| C | GLY85 | |
| C | GLN88 | |
| C | SER107 | |
| C | ALA124 | |
| C | SER149 | |
| A | LYS46 | |
| C | HIS174 | |
| D | VAL20 | |
| D | ASP41 | |
| D | LYS46 | |
| D | TYR71 | |
| D | GLY85 | |
| D | GLN88 | |
| D | SER107 | |
| D | ALA124 | |
| D | SER149 | |
| A | TYR71 | |
| D | HIS174 | |
| E | VAL20 | |
| E | ASP41 | |
| E | LYS46 | |
| E | TYR71 | |
| E | GLY85 | |
| E | GLN88 | |
| E | SER107 | |
| E | ALA124 | |
| E | SER149 | |
| A | GLY85 | |
| E | HIS174 | |
| F | VAL20 | |
| F | ASP41 | |
| F | LYS46 | |
| F | TYR71 | |
| F | GLY85 | |
| F | GLN88 | |
| F | SER107 | |
| F | ALA124 | |
| F | SER149 | |
| A | GLN88 | |
| F | HIS174 | |
| A | SER107 | |
| A | ALA124 | |
| A | SER149 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000269|Ref.8, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | ARG94 | |
| B | ARG94 | |
| C | ARG94 | |
| D | ARG94 | |
| E | ARG94 | |
| F | ARG94 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305|Ref.8, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | ASN126 | |
| B | ASN126 | |
| C | ASN126 | |
| D | ASN126 | |
| E | ASN126 | |
| F | ASN126 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305|Ref.8, ECO:0007744|PDB:6J9S, ECO:0007744|PDB:6J9T, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | ASP154 | |
| B | ASP154 | |
| C | ASP154 | |
| D | ASP154 | |
| E | ASP154 | |
| F | ASP154 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000269|Ref.8, ECO:0007744|PDB:6J9T, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | ARG159 | |
| B | ARG159 | |
| C | ARG159 | |
| D | ARG159 | |
| E | ARG159 | |
| F | ARG159 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|Ref.8, ECO:0007744|PDB:6J9T |
| Chain | Residue | Details |
| A | ARG171 | |
| B | ARG171 | |
| C | ARG171 | |
| D | ARG171 | |
| E | ARG171 | |
| F | ARG171 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000269|Ref.8, ECO:0007744|PDB:6J9S, ECO:0007744|PDB:6J9U |
| Chain | Residue | Details |
| A | THR235 | |
| B | THR235 | |
| C | THR235 | |
| D | THR235 | |
| E | THR235 | |
| F | THR235 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000255|HAMAP-Rule:MF_00488 |
| Chain | Residue | Details |
| A | TYR226 | |
| B | TYR226 | |
| C | TYR226 | |
| D | TYR226 | |
| E | TYR226 | |
| F | TYR226 |
