6JML
Re-refined structure of R-state L-lactate dehydrogenase fromLactobacillus casei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.382, 82.893, 179.771 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 34.100 - 2.300 |
| R-factor | 0.19535 |
| Rwork | 0.194 |
| R-free | 0.22166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zqz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.747 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.509 |
| Number of reflections | 168255 | 7480 |
| <I/σ(I)> | 7.1 | 0.9 |
| Completeness [%] | 99.9 | 93.3 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | ammonium sulfate, sodium citrate, potassium sodium tartrate |
