Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6I7P

Crystal structure of the full-length Zika virus NS5 protein (Human isolate Z1106033)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003968	molecular_function	RNA-directed RNA polymerase activity
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	methyltransferase cap1 activity
A	0005524	molecular_function	ATP binding
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
A	0039694	biological_process	viral RNA genome replication
B	0003968	molecular_function	RNA-directed RNA polymerase activity
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0004483	molecular_function	methyltransferase cap1 activity
B	0005524	molecular_function	ATP binding
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation
B	0039694	biological_process	viral RNA genome replication
C	0003968	molecular_function	RNA-directed RNA polymerase activity
C	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
C	0004483	molecular_function	methyltransferase cap1 activity
C	0005524	molecular_function	ATP binding
C	0008168	molecular_function	methyltransferase activity
C	0032259	biological_process	methylation
C	0039694	biological_process	viral RNA genome replication
D	0003968	molecular_function	RNA-directed RNA polymerase activity
D	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
D	0004483	molecular_function	methyltransferase cap1 activity
D	0005524	molecular_function	ATP binding
D	0008168	molecular_function	methyltransferase activity
D	0032259	biological_process	methylation
D	0039694	biological_process	viral RNA genome replication
E	0003968	molecular_function	RNA-directed RNA polymerase activity
E	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
E	0004483	molecular_function	methyltransferase cap1 activity
E	0005524	molecular_function	ATP binding
E	0008168	molecular_function	methyltransferase activity
E	0032259	biological_process	methylation
E	0039694	biological_process	viral RNA genome replication
F	0003968	molecular_function	RNA-directed RNA polymerase activity
F	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
F	0004483	molecular_function	methyltransferase cap1 activity
F	0005524	molecular_function	ATP binding
F	0008168	molecular_function	methyltransferase activity
F	0032259	biological_process	methylation
F	0039694	biological_process	viral RNA genome replication

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue SAH A 1001

Chain	Residue
A	SER56
A	HIS110
A	GLU111
A	VAL130
A	ASP131
A	VAL132
A	ASP146
A	ASP79
A	GLY81
A	CYS82
A	GLY85
A	GLY86
A	TRP87
A	THR104
A	LYS105

site_id	AC2
Number of Residues	2
Details	binding site for residue PO4 A 1002

Chain	Residue
A	LYS462
A	SER712

site_id	AC3
Number of Residues	3
Details	binding site for residue PO4 A 1003

Chain	Residue
A	ARG57
A	ARG213
B	LYS45

site_id	AC4
Number of Residues	3
Details	binding site for residue ZN A 1004

Chain	Residue
A	HIS714
A	CYS730
A	CYS849

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN A 1005

Chain	Residue
A	GLU439
A	HIS443
A	CYS448
A	CYS451

site_id	AC6
Number of Residues	14
Details	binding site for residue SAH B 1001

Chain	Residue
B	SER56
B	GLY58
B	GLY81
B	CYS82
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	HIS110
B	GLU111
B	VAL130
B	ASP131
B	VAL132
B	ASP146

site_id	AC7
Number of Residues	4
Details	binding site for residue PO4 B 1002

Chain	Residue
A	LYS45
B	ARG41
B	ARG57
B	ARG213

site_id	AC8
Number of Residues	2
Details	binding site for residue PO4 B 1003

Chain	Residue
B	HIS855
B	ARG856

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN B 1004

Chain	Residue
B	HIS714
B	ASN716
B	CYS730
B	CYS849

site_id	AD1
Number of Residues	12
Details	binding site for residue SAH C 1001

Chain	Residue
C	SER56
C	GLY58
C	GLY81
C	CYS82
C	GLY83
C	TRP87
C	THR104
C	LYS105
C	HIS110
C	VAL130
C	ASP131
C	ASP146

site_id	AD2
Number of Residues	2
Details	binding site for residue PO4 C 1002

Chain	Residue
C	LYS76
C	LYS101

site_id	AD3
Number of Residues	4
Details	binding site for residue ZN C 1003

Chain	Residue
C	HIS714
C	ASN716
C	CYS730
C	CYS849

site_id	AD4
Number of Residues	4
Details	binding site for residue ZN C 1004

Chain	Residue
C	GLU439
C	HIS443
C	CYS448
C	CYS451

site_id	AD5
Number of Residues	11
Details	binding site for residue SAH D 1001

Chain	Residue
D	SER56
D	GLY58
D	GLY81
D	CYS82
D	GLY86
D	TRP87
D	THR104
D	LYS105
D	VAL130
D	ASP131
D	ASP146

site_id	AD6
Number of Residues	1
Details	binding site for residue PO4 D 1002

Chain	Residue
D	LYS101

site_id	AD7
Number of Residues	1
Details	binding site for residue PO4 D 1003

Chain	Residue
D	ARG125

site_id	AD8
Number of Residues	3
Details	binding site for residue PO4 D 1004

Chain	Residue
D	LYS470
D	ARG473
D	LYS691

site_id	AD9
Number of Residues	3
Details	binding site for residue PO4 D 1005

Chain	Residue
D	SER712
D	ARG731
D	ARG739

site_id	AE1
Number of Residues	3
Details	binding site for residue ZN D 1006

Chain	Residue
D	HIS714
D	CYS730
D	CYS849

site_id	AE2
Number of Residues	5
Details	binding site for residue ZN D 1007

Chain	Residue
D	CYS451
D	GLU439
D	HIS443
D	CYS448
D	SER450

site_id	AE3
Number of Residues	11
Details	binding site for residue SAH E 1001

Chain	Residue
E	SER56
E	GLY81
E	CYS82
E	GLY85
E	GLY86
E	TRP87
E	THR104
E	LYS105
E	HIS110
E	VAL132
E	ASP146

site_id	AE4
Number of Residues	3
Details	binding site for residue PO4 E 1002

Chain	Residue
E	ARG57
E	ARG213
F	LYS45

site_id	AE5
Number of Residues	1
Details	binding site for residue PO4 E 1003

Chain	Residue
E	HIS855

site_id	AE6
Number of Residues	3
Details	binding site for residue ZN E 1004

Chain	Residue
E	HIS714
E	CYS730
E	CYS849

site_id	AE7
Number of Residues	6
Details	binding site for residue ZN E 1005

Chain	Residue
E	GLU439
E	HIS443
E	CYS448
E	GLN449
E	SER450
E	CYS451

site_id	AE8
Number of Residues	12
Details	binding site for residue SAH F 1001

Chain	Residue
F	SER56
F	GLY81
F	CYS82
F	GLY86
F	TRP87
F	THR104
F	LYS105
F	HIS110
F	VAL130
F	PHE133
F	ASP146
F	ILE147

site_id	AE9
Number of Residues	1
Details	binding site for residue PO4 F 1002

Chain	Residue
F	SER712

site_id	AF1
Number of Residues	1
Details	binding site for residue PO4 F 1003

Chain	Residue
F	LYS691

site_id	AF2
Number of Residues	3
Details	binding site for residue PO4 F 1004

Chain	Residue
F	LYS76
F	GLU99
F	LYS101

site_id	AF3
Number of Residues	2
Details	binding site for residue PO4 F 1005

Chain	Residue
F	LYS101
F	ARG125

site_id	AF4
Number of Residues	5
Details	binding site for residue ZN F 1006

Chain	Residue
F	HIS714
F	ASN716
F	CYS730
F	LEU841
F	CYS849

site_id	AF5
Number of Residues	4
Details	binding site for residue ZN F 1007

Chain	Residue
F	GLU439
F	HIS443
F	CYS448
F	CYS451

site_id	AF6
Number of Residues	13
Details	binding site for Di-peptide GLU B 417 and TRP B 479

Chain	Residue
B	GLY411
B	ALA412
B	ILE413
B	PHE414
B	GLU415
B	GLU416
B	LYS418
B	GLU419
B	MET478
B	LEU480
B	GLY481
B	ALA482
B	ARG483

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI2
Number of Residues	18
Details	Region: {"description":"SUMO-interacting motif (SIM)","evidences":[{"source":"PubMed","id":"32699085","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	36
Details	Motif: {"description":"Nuclear localization signal (NLS)","evidences":[{"source":"UniProtKB","id":"Q32ZE1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	24
Details	Active site: {"description":"For 2'-O-MTase activity","evidences":[{"source":"UniProtKB","id":"Q6YMS4","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	84
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27866982","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27633330","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27866982","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28031359","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	42
Details	Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	12
Details	Site: {"description":"mRNA cap binding; via carbonyl oxygen","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	18
Details	Site: {"description":"Essential for 2'-O-methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	6
Details	Site: {"description":"Essential for 2'-O-methyltransferase and N-7 methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P03314","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)