Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6H77

E1 enzyme for ubiquitin like protein activation in complex with UBL

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
C	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005783	cellular_component	endoplasmic reticulum
Q	0007420	biological_process	brain development
Q	0033146	biological_process	regulation of intracellular estrogen receptor signaling pathway
Q	0034976	biological_process	response to endoplasmic reticulum stress
Q	0042308	biological_process	negative regulation of protein import into nucleus
Q	0043066	biological_process	negative regulation of apoptotic process
Q	0061709	biological_process	reticulophagy
Q	0071569	biological_process	protein ufmylation
Q	1990592	biological_process	protein K69-linked ufmylation
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005783	cellular_component	endoplasmic reticulum
R	0007420	biological_process	brain development
R	0033146	biological_process	regulation of intracellular estrogen receptor signaling pathway
R	0034976	biological_process	response to endoplasmic reticulum stress
R	0042308	biological_process	negative regulation of protein import into nucleus
R	0043066	biological_process	negative regulation of apoptotic process
R	0061709	biological_process	reticulophagy
R	0071569	biological_process	protein ufmylation
R	1990592	biological_process	protein K69-linked ufmylation
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005737	cellular_component	cytoplasm
S	0005783	cellular_component	endoplasmic reticulum
S	0007420	biological_process	brain development
S	0033146	biological_process	regulation of intracellular estrogen receptor signaling pathway
S	0034976	biological_process	response to endoplasmic reticulum stress
S	0042308	biological_process	negative regulation of protein import into nucleus
S	0043066	biological_process	negative regulation of apoptotic process
S	0061709	biological_process	reticulophagy
S	0071569	biological_process	protein ufmylation
S	1990592	biological_process	protein K69-linked ufmylation
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005737	cellular_component	cytoplasm
T	0005783	cellular_component	endoplasmic reticulum
T	0007420	biological_process	brain development
T	0033146	biological_process	regulation of intracellular estrogen receptor signaling pathway
T	0034976	biological_process	response to endoplasmic reticulum stress
T	0042308	biological_process	negative regulation of protein import into nucleus
T	0043066	biological_process	negative regulation of apoptotic process
T	0061709	biological_process	reticulophagy
T	0071569	biological_process	protein ufmylation
T	1990592	biological_process	protein K69-linked ufmylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	29
Details	binding site for residue ATP A 401

Chain	Residue
A	GLY80
A	TYR149
A	ASN150
A	ILE151
A	THR152
A	CYS181
A	ASP183
A	ASN184
A	MG402
A	MG404
A	EDO411
A	GLY82
A	HOH515
A	HOH521
A	HOH523
A	HOH536
A	HOH539
A	HOH554
A	HOH570
A	HOH571
A	HOH575
B	ARG55
A	GLY83
A	ASP104
A	TYR105
A	ASP106
A	ASN112
A	ARG115
A	LYS127

site_id	AC2
Number of Residues	4
Details	binding site for residue MG A 402

Chain	Residue
A	ASP183
A	ATP401
A	HOH536
A	HOH570

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 403

Chain	Residue
A	CYS226
A	CYS229
A	CYS303
A	CYS308

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 404

Chain	Residue
A	ASP106
A	ATP401
A	HOH506
A	HOH521
A	HOH523
A	HOH571

site_id	AC5
Number of Residues	4
Details	binding site for residue EDO A 405

Chain	Residue
A	GLU90
A	PHE117
A	GLN119
B	PHE117

site_id	AC6
Number of Residues	5
Details	binding site for residue EDO A 406

Chain	Residue
A	GLU90
A	PHE117
B	GLU90
B	LEU116
B	PHE117

site_id	AC7
Number of Residues	7
Details	binding site for residue EDO A 407

Chain	Residue
A	SER213
A	TYR282
A	GLY284
A	ASN286
A	HOH541
A	HOH576
T	PRO78

site_id	AC8
Number of Residues	4
Details	binding site for residue EDO A 408

Chain	Residue
A	GLN289
A	EDO409
B	THR294
B	HOH508

site_id	AC9
Number of Residues	7
Details	binding site for residue EDO A 409

Chain	Residue
A	PHE291
A	PHE292
A	EDO408
B	PHE291
B	PHE292
B	HOH508
B	HOH514

site_id	AD1
Number of Residues	2
Details	binding site for residue EDO A 410

Chain	Residue
A	THR294
T	HOH205

site_id	AD2
Number of Residues	10
Details	binding site for residue EDO A 411

Chain	Residue
A	GLY82
A	GLY83
A	SER86
A	ASN112
A	ARG115
A	LEU116
A	PHE118
A	LYS127
A	ATP401
A	HOH538

site_id	AD3
Number of Residues	6
Details	binding site for residue EDO A 412

Chain	Residue
A	HIS133
A	ARG136
A	HOH550
A	HOH569
C	ALA123
C	GLY124

site_id	AD4
Number of Residues	4
Details	binding site for residue EDO A 413

Chain	Residue
A	GLN158
D	ARG306
D	ARG309
D	GLU313

site_id	AD5
Number of Residues	5
Details	binding site for residue EDO A 414

Chain	Residue
A	GLU145
A	HIS147
A	HIS159
A	ARG163
A	HOH589

site_id	AD6
Number of Residues	30
Details	binding site for residue ATP B 401

Chain	Residue
B	GLY82
B	GLY83
B	ASP104
B	TYR105
B	ASP106
B	ASN112
B	ARG115
B	LYS127
B	TYR149
B	ASN150
B	ILE151
B	THR152
B	VAL182
B	ASP183
B	ASN184
B	MG402
B	MG404
B	HOH506
B	HOH516
B	HOH517
B	HOH532
B	HOH537
B	HOH543
B	HOH555
B	HOH559
B	HOH565
B	HOH568
B	HOH580
A	ARG55
B	GLY80

site_id	AD7
Number of Residues	4
Details	binding site for residue MG B 402

Chain	Residue
B	ASP183
B	ATP401
B	HOH532
B	HOH580

site_id	AD8
Number of Residues	4
Details	binding site for residue ZN B 403

Chain	Residue
B	CYS226
B	CYS229
B	CYS303
B	CYS308

site_id	AD9
Number of Residues	6
Details	binding site for residue MG B 404

Chain	Residue
B	ASP106
B	ATP401
B	HOH506
B	HOH507
B	HOH516
B	HOH568

site_id	AE1
Number of Residues	7
Details	binding site for residue EDO B 405

Chain	Residue
B	GLY206
B	VAL207
B	SER208
B	GLY214
B	HIS215
B	EDO406
Q	HOH109

site_id	AE2
Number of Residues	10
Details	binding site for residue EDO B 406

Chain	Residue
B	SER213
B	GLY214
B	TYR282
B	ASN286
B	PRO293
B	EDO405
B	HOH502
B	HOH552
Q	SER12
Q	PRO78

site_id	AE3
Number of Residues	2
Details	binding site for residue EDO B 407

Chain	Residue
B	GLU132
B	VAL146

site_id	AE4
Number of Residues	2
Details	binding site for residue EDO B 408

Chain	Residue
B	GLU155
B	ASN156

site_id	AE5
Number of Residues	4
Details	binding site for residue EDO B 409

Chain	Residue
B	ILE220
B	PRO221
B	GLU223
B	TYR315

site_id	AE6
Number of Residues	4
Details	binding site for residue EDO B 410

Chain	Residue
B	ASP183
B	PHE185
B	ARG188
B	GLU241

site_id	AE7
Number of Residues	3
Details	binding site for residue EDO B 411

Chain	Residue
B	PRO301
B	GLU313
B	LYS316

site_id	AE8
Number of Residues	29
Details	binding site for residue ATP C 401

Chain	Residue
C	GLY80
C	GLY82
C	GLY83
C	ASP104
C	TYR105
C	ASP106
C	ASN112
C	ARG115
C	LYS127
C	ASN150
C	ILE151
C	THR152
C	CYS181
C	VAL182
C	ASP183
C	ASN184
C	ALA187
C	MG402
C	MG404
C	EDO408
C	HOH507
C	HOH523
C	HOH525
C	HOH530
C	HOH547
C	HOH551
C	HOH552
C	HOH560
D	ARG55

site_id	AE9
Number of Residues	4
Details	binding site for residue MG C 402

Chain	Residue
C	ASP183
C	ATP401
C	HOH523
C	HOH560

site_id	AF1
Number of Residues	4
Details	binding site for residue ZN C 403

Chain	Residue
C	CYS226
C	CYS229
C	CYS303
C	CYS308

site_id	AF2
Number of Residues	6
Details	binding site for residue MG C 404

Chain	Residue
C	ASP106
C	ATP401
C	HOH501
C	HOH507
C	HOH551
C	HOH552

site_id	AF3
Number of Residues	5
Details	binding site for residue EDO C 405

Chain	Residue
C	GLU90
C	LEU116
C	PHE117
D	GLU90
D	PHE117

site_id	AF4
Number of Residues	5
Details	binding site for residue EDO C 406

Chain	Residue
C	GLY206
C	GLY214
C	HIS215
C	EDO407
R	PRO78

site_id	AF5
Number of Residues	7
Details	binding site for residue EDO C 407

Chain	Residue
C	TYR282
C	ASN286
C	PRO293
C	EDO406
C	HOH549
R	SER12
R	PRO78

site_id	AF6
Number of Residues	11
Details	binding site for residue EDO C 408

Chain	Residue
C	GLY82
C	GLY83
C	SER86
C	ASN112
C	ARG115
C	LEU116
C	PHE118
C	LYS127
C	ATP401
C	HOH529
C	HOH540

site_id	AF7
Number of Residues	5
Details	binding site for residue EDO C 409

Chain	Residue
C	PHE291
C	PHE292
C	HOH506
D	PHE291
D	PHE292

site_id	AF8
Number of Residues	3
Details	binding site for residue EDO C 410

Chain	Residue
B	GLN158
C	ARG306
C	ARG309

site_id	AF9
Number of Residues	5
Details	binding site for residue PEG C 411

Chain	Residue
C	ASP183
C	ARG188
C	VAL207
C	GLU209
C	ALA251

site_id	AG1
Number of Residues	31
Details	binding site for residue ATP D 401

Chain	Residue
C	ARG55
D	GLY80
D	GLY82
D	GLY83
D	ASP104
D	TYR105
D	ASP106
D	ASN112
D	ARG115
D	LYS127
D	TYR149
D	ASN150
D	ILE151
D	THR152
D	CYS181
D	VAL182
D	ASP183
D	ASN184
D	ALA187
D	MG402
D	MG404
D	EDO406
D	PEG416
D	HOH505
D	HOH513
D	HOH526
D	HOH537
D	HOH545
D	HOH551
D	HOH558
D	HOH564

site_id	AG2
Number of Residues	4
Details	binding site for residue MG D 402

Chain	Residue
D	ASP183
D	ATP401
D	HOH537
D	HOH558

site_id	AG3
Number of Residues	4
Details	binding site for residue ZN D 403

Chain	Residue
D	CYS226
D	CYS229
D	CYS303
D	CYS308

site_id	AG4
Number of Residues	6
Details	binding site for residue MG D 404

Chain	Residue
D	ASP106
D	ATP401
D	HOH505
D	HOH507
D	HOH513
D	HOH551

site_id	AG5
Number of Residues	5
Details	binding site for residue EDO D 405

Chain	Residue
C	GLU90
C	PHE117
D	GLU90
D	PHE117
D	GLN119

site_id	AG6
Number of Residues	10
Details	binding site for residue EDO D 406

Chain	Residue
D	GLY82
D	GLY83
D	SER86
D	ASN112
D	ARG115
D	LEU116
D	PHE118
D	LYS127
D	ATP401
D	HOH533

site_id	AG7
Number of Residues	3
Details	binding site for residue EDO D 407

Chain	Residue
D	GLU155
D	ASN156
D	HIS159

site_id	AG8
Number of Residues	3
Details	binding site for residue EDO D 408

Chain	Residue
A	ASP304
D	THR152
D	THR190

site_id	AG9
Number of Residues	3
Details	binding site for residue EDO D 409

Chain	Residue
D	PRO301
D	GLU313
D	LYS316

site_id	AH1
Number of Residues	4
Details	binding site for residue EDO D 410

Chain	Residue
D	GLY214
D	HIS215
D	EDO411
S	PRO78

site_id	AH2
Number of Residues	7
Details	binding site for residue EDO D 411

Chain	Residue
D	SER213
D	TYR282
D	ASN286
D	PRO293
D	EDO410
D	HOH567
S	PRO78

site_id	AH3
Number of Residues	3
Details	binding site for residue EDO D 412

Chain	Residue
D	GLU186
D	ILE239
D	GLU241

site_id	AH4
Number of Residues	5
Details	binding site for residue EDO D 413

Chain	Residue
D	MET295
D	SER296
S	ASN52
S	GLY54
S	ARG75

site_id	AH5
Number of Residues	5
Details	binding site for residue EDO D 414

Chain	Residue
A	VAL154
A	PHE157
D	ASP304
D	ARG309
D	HOH534

site_id	AH6
Number of Residues	6
Details	binding site for residue PEG D 415

Chain	Residue
B	GLU132
B	HIS133
B	ARG136
C	GLY36
D	HIS121
D	ALA123

site_id	AH7
Number of Residues	11
Details	binding site for residue PEG D 416

Chain	Residue
D	GLY83
D	VAL84
D	CYS181
D	VAL182
D	ARG188
D	GLY206
D	VAL207
D	ATP401
D	HOH508
D	HOH511
D	HOH584

site_id	AH8
Number of Residues	2
Details	binding site for residue EDO R 101

Chain	Residue
R	ASP53
T	SER72

site_id	AH9
Number of Residues	5
Details	binding site for residue EDO T 101

Chain	Residue
A	MET295
A	SER296
T	ASN52
T	GLY54
T	ARG75

Functional Information from PROSITE/UniProt

site_id	PS00065
Number of Residues	29
Details	D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. VAIVGvGGVGsvtaemltrcgigkLLlFD

Chain	Residue	Details
A	VAL76-ASP104

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	48
Details	Motif: {"description":"UFM1-interacting sequence (UIS)","evidences":[{"source":"PubMed","id":"26929408","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27545674","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34588452","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H77","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IAA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H77","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)","evidences":[{"source":"PubMed","id":"25219498","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)