6H77
E1 enzyme for ubiquitin like protein activation in complex with UBL
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| B | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| C | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| D | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| Q | 0005515 | molecular_function | protein binding |
| Q | 0005634 | cellular_component | nucleus |
| Q | 0005737 | cellular_component | cytoplasm |
| Q | 0005783 | cellular_component | endoplasmic reticulum |
| Q | 0007420 | biological_process | brain development |
| Q | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| Q | 0034976 | biological_process | response to endoplasmic reticulum stress |
| Q | 0042308 | biological_process | negative regulation of protein import into nucleus |
| Q | 0043066 | biological_process | negative regulation of apoptotic process |
| Q | 0061709 | biological_process | reticulophagy |
| Q | 0071569 | biological_process | protein ufmylation |
| Q | 1990592 | biological_process | protein K69-linked ufmylation |
| R | 0005515 | molecular_function | protein binding |
| R | 0005634 | cellular_component | nucleus |
| R | 0005737 | cellular_component | cytoplasm |
| R | 0005783 | cellular_component | endoplasmic reticulum |
| R | 0007420 | biological_process | brain development |
| R | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| R | 0034976 | biological_process | response to endoplasmic reticulum stress |
| R | 0042308 | biological_process | negative regulation of protein import into nucleus |
| R | 0043066 | biological_process | negative regulation of apoptotic process |
| R | 0061709 | biological_process | reticulophagy |
| R | 0071569 | biological_process | protein ufmylation |
| R | 1990592 | biological_process | protein K69-linked ufmylation |
| S | 0005515 | molecular_function | protein binding |
| S | 0005634 | cellular_component | nucleus |
| S | 0005737 | cellular_component | cytoplasm |
| S | 0005783 | cellular_component | endoplasmic reticulum |
| S | 0007420 | biological_process | brain development |
| S | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| S | 0034976 | biological_process | response to endoplasmic reticulum stress |
| S | 0042308 | biological_process | negative regulation of protein import into nucleus |
| S | 0043066 | biological_process | negative regulation of apoptotic process |
| S | 0061709 | biological_process | reticulophagy |
| S | 0071569 | biological_process | protein ufmylation |
| S | 1990592 | biological_process | protein K69-linked ufmylation |
| T | 0005515 | molecular_function | protein binding |
| T | 0005634 | cellular_component | nucleus |
| T | 0005737 | cellular_component | cytoplasm |
| T | 0005783 | cellular_component | endoplasmic reticulum |
| T | 0007420 | biological_process | brain development |
| T | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| T | 0034976 | biological_process | response to endoplasmic reticulum stress |
| T | 0042308 | biological_process | negative regulation of protein import into nucleus |
| T | 0043066 | biological_process | negative regulation of apoptotic process |
| T | 0061709 | biological_process | reticulophagy |
| T | 0071569 | biological_process | protein ufmylation |
| T | 1990592 | biological_process | protein K69-linked ufmylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 29 |
| Details | binding site for residue ATP A 401 |
| Chain | Residue |
| A | GLY80 |
| A | TYR149 |
| A | ASN150 |
| A | ILE151 |
| A | THR152 |
| A | CYS181 |
| A | ASP183 |
| A | ASN184 |
| A | MG402 |
| A | MG404 |
| A | EDO411 |
| A | GLY82 |
| A | HOH515 |
| A | HOH521 |
| A | HOH523 |
| A | HOH536 |
| A | HOH539 |
| A | HOH554 |
| A | HOH570 |
| A | HOH571 |
| A | HOH575 |
| B | ARG55 |
| A | GLY83 |
| A | ASP104 |
| A | TYR105 |
| A | ASP106 |
| A | ASN112 |
| A | ARG115 |
| A | LYS127 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue MG A 402 |
| Chain | Residue |
| A | ASP183 |
| A | ATP401 |
| A | HOH536 |
| A | HOH570 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 403 |
| Chain | Residue |
| A | CYS226 |
| A | CYS229 |
| A | CYS303 |
| A | CYS308 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 404 |
| Chain | Residue |
| A | ASP106 |
| A | ATP401 |
| A | HOH506 |
| A | HOH521 |
| A | HOH523 |
| A | HOH571 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 405 |
| Chain | Residue |
| A | GLU90 |
| A | PHE117 |
| A | GLN119 |
| B | PHE117 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 406 |
| Chain | Residue |
| A | GLU90 |
| A | PHE117 |
| B | GLU90 |
| B | LEU116 |
| B | PHE117 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 407 |
| Chain | Residue |
| A | SER213 |
| A | TYR282 |
| A | GLY284 |
| A | ASN286 |
| A | HOH541 |
| A | HOH576 |
| T | PRO78 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 408 |
| Chain | Residue |
| A | GLN289 |
| A | EDO409 |
| B | THR294 |
| B | HOH508 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 409 |
| Chain | Residue |
| A | PHE291 |
| A | PHE292 |
| A | EDO408 |
| B | PHE291 |
| B | PHE292 |
| B | HOH508 |
| B | HOH514 |
| site_id | AD1 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 410 |
| Chain | Residue |
| A | THR294 |
| T | HOH205 |
| site_id | AD2 |
| Number of Residues | 10 |
| Details | binding site for residue EDO A 411 |
| Chain | Residue |
| A | GLY82 |
| A | GLY83 |
| A | SER86 |
| A | ASN112 |
| A | ARG115 |
| A | LEU116 |
| A | PHE118 |
| A | LYS127 |
| A | ATP401 |
| A | HOH538 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 412 |
| Chain | Residue |
| A | HIS133 |
| A | ARG136 |
| A | HOH550 |
| A | HOH569 |
| C | ALA123 |
| C | GLY124 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 413 |
| Chain | Residue |
| A | GLN158 |
| D | ARG306 |
| D | ARG309 |
| D | GLU313 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 414 |
| Chain | Residue |
| A | GLU145 |
| A | HIS147 |
| A | HIS159 |
| A | ARG163 |
| A | HOH589 |
| site_id | AD6 |
| Number of Residues | 30 |
| Details | binding site for residue ATP B 401 |
| Chain | Residue |
| B | GLY82 |
| B | GLY83 |
| B | ASP104 |
| B | TYR105 |
| B | ASP106 |
| B | ASN112 |
| B | ARG115 |
| B | LYS127 |
| B | TYR149 |
| B | ASN150 |
| B | ILE151 |
| B | THR152 |
| B | VAL182 |
| B | ASP183 |
| B | ASN184 |
| B | MG402 |
| B | MG404 |
| B | HOH506 |
| B | HOH516 |
| B | HOH517 |
| B | HOH532 |
| B | HOH537 |
| B | HOH543 |
| B | HOH555 |
| B | HOH559 |
| B | HOH565 |
| B | HOH568 |
| B | HOH580 |
| A | ARG55 |
| B | GLY80 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue MG B 402 |
| Chain | Residue |
| B | ASP183 |
| B | ATP401 |
| B | HOH532 |
| B | HOH580 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 403 |
| Chain | Residue |
| B | CYS226 |
| B | CYS229 |
| B | CYS303 |
| B | CYS308 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue MG B 404 |
| Chain | Residue |
| B | ASP106 |
| B | ATP401 |
| B | HOH506 |
| B | HOH507 |
| B | HOH516 |
| B | HOH568 |
| site_id | AE1 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 405 |
| Chain | Residue |
| B | GLY206 |
| B | VAL207 |
| B | SER208 |
| B | GLY214 |
| B | HIS215 |
| B | EDO406 |
| Q | HOH109 |
| site_id | AE2 |
| Number of Residues | 10 |
| Details | binding site for residue EDO B 406 |
| Chain | Residue |
| B | SER213 |
| B | GLY214 |
| B | TYR282 |
| B | ASN286 |
| B | PRO293 |
| B | EDO405 |
| B | HOH502 |
| B | HOH552 |
| Q | SER12 |
| Q | PRO78 |
| site_id | AE3 |
| Number of Residues | 2 |
| Details | binding site for residue EDO B 407 |
| Chain | Residue |
| B | GLU132 |
| B | VAL146 |
| site_id | AE4 |
| Number of Residues | 2 |
| Details | binding site for residue EDO B 408 |
| Chain | Residue |
| B | GLU155 |
| B | ASN156 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 409 |
| Chain | Residue |
| B | ILE220 |
| B | PRO221 |
| B | GLU223 |
| B | TYR315 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 410 |
| Chain | Residue |
| B | ASP183 |
| B | PHE185 |
| B | ARG188 |
| B | GLU241 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 411 |
| Chain | Residue |
| B | PRO301 |
| B | GLU313 |
| B | LYS316 |
| site_id | AE8 |
| Number of Residues | 29 |
| Details | binding site for residue ATP C 401 |
| Chain | Residue |
| C | GLY80 |
| C | GLY82 |
| C | GLY83 |
| C | ASP104 |
| C | TYR105 |
| C | ASP106 |
| C | ASN112 |
| C | ARG115 |
| C | LYS127 |
| C | ASN150 |
| C | ILE151 |
| C | THR152 |
| C | CYS181 |
| C | VAL182 |
| C | ASP183 |
| C | ASN184 |
| C | ALA187 |
| C | MG402 |
| C | MG404 |
| C | EDO408 |
| C | HOH507 |
| C | HOH523 |
| C | HOH525 |
| C | HOH530 |
| C | HOH547 |
| C | HOH551 |
| C | HOH552 |
| C | HOH560 |
| D | ARG55 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue MG C 402 |
| Chain | Residue |
| C | ASP183 |
| C | ATP401 |
| C | HOH523 |
| C | HOH560 |
| site_id | AF1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN C 403 |
| Chain | Residue |
| C | CYS226 |
| C | CYS229 |
| C | CYS303 |
| C | CYS308 |
| site_id | AF2 |
| Number of Residues | 6 |
| Details | binding site for residue MG C 404 |
| Chain | Residue |
| C | ASP106 |
| C | ATP401 |
| C | HOH501 |
| C | HOH507 |
| C | HOH551 |
| C | HOH552 |
| site_id | AF3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 405 |
| Chain | Residue |
| C | GLU90 |
| C | LEU116 |
| C | PHE117 |
| D | GLU90 |
| D | PHE117 |
| site_id | AF4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 406 |
| Chain | Residue |
| C | GLY206 |
| C | GLY214 |
| C | HIS215 |
| C | EDO407 |
| R | PRO78 |
| site_id | AF5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO C 407 |
| Chain | Residue |
| C | TYR282 |
| C | ASN286 |
| C | PRO293 |
| C | EDO406 |
| C | HOH549 |
| R | SER12 |
| R | PRO78 |
| site_id | AF6 |
| Number of Residues | 11 |
| Details | binding site for residue EDO C 408 |
| Chain | Residue |
| C | GLY82 |
| C | GLY83 |
| C | SER86 |
| C | ASN112 |
| C | ARG115 |
| C | LEU116 |
| C | PHE118 |
| C | LYS127 |
| C | ATP401 |
| C | HOH529 |
| C | HOH540 |
| site_id | AF7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 409 |
| Chain | Residue |
| C | PHE291 |
| C | PHE292 |
| C | HOH506 |
| D | PHE291 |
| D | PHE292 |
| site_id | AF8 |
| Number of Residues | 3 |
| Details | binding site for residue EDO C 410 |
| Chain | Residue |
| B | GLN158 |
| C | ARG306 |
| C | ARG309 |
| site_id | AF9 |
| Number of Residues | 5 |
| Details | binding site for residue PEG C 411 |
| Chain | Residue |
| C | ASP183 |
| C | ARG188 |
| C | VAL207 |
| C | GLU209 |
| C | ALA251 |
| site_id | AG1 |
| Number of Residues | 31 |
| Details | binding site for residue ATP D 401 |
| Chain | Residue |
| C | ARG55 |
| D | GLY80 |
| D | GLY82 |
| D | GLY83 |
| D | ASP104 |
| D | TYR105 |
| D | ASP106 |
| D | ASN112 |
| D | ARG115 |
| D | LYS127 |
| D | TYR149 |
| D | ASN150 |
| D | ILE151 |
| D | THR152 |
| D | CYS181 |
| D | VAL182 |
| D | ASP183 |
| D | ASN184 |
| D | ALA187 |
| D | MG402 |
| D | MG404 |
| D | EDO406 |
| D | PEG416 |
| D | HOH505 |
| D | HOH513 |
| D | HOH526 |
| D | HOH537 |
| D | HOH545 |
| D | HOH551 |
| D | HOH558 |
| D | HOH564 |
| site_id | AG2 |
| Number of Residues | 4 |
| Details | binding site for residue MG D 402 |
| Chain | Residue |
| D | ASP183 |
| D | ATP401 |
| D | HOH537 |
| D | HOH558 |
| site_id | AG3 |
| Number of Residues | 4 |
| Details | binding site for residue ZN D 403 |
| Chain | Residue |
| D | CYS226 |
| D | CYS229 |
| D | CYS303 |
| D | CYS308 |
| site_id | AG4 |
| Number of Residues | 6 |
| Details | binding site for residue MG D 404 |
| Chain | Residue |
| D | ASP106 |
| D | ATP401 |
| D | HOH505 |
| D | HOH507 |
| D | HOH513 |
| D | HOH551 |
| site_id | AG5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO D 405 |
| Chain | Residue |
| C | GLU90 |
| C | PHE117 |
| D | GLU90 |
| D | PHE117 |
| D | GLN119 |
| site_id | AG6 |
| Number of Residues | 10 |
| Details | binding site for residue EDO D 406 |
| Chain | Residue |
| D | GLY82 |
| D | GLY83 |
| D | SER86 |
| D | ASN112 |
| D | ARG115 |
| D | LEU116 |
| D | PHE118 |
| D | LYS127 |
| D | ATP401 |
| D | HOH533 |
| site_id | AG7 |
| Number of Residues | 3 |
| Details | binding site for residue EDO D 407 |
| Chain | Residue |
| D | GLU155 |
| D | ASN156 |
| D | HIS159 |
| site_id | AG8 |
| Number of Residues | 3 |
| Details | binding site for residue EDO D 408 |
| Chain | Residue |
| A | ASP304 |
| D | THR152 |
| D | THR190 |
| site_id | AG9 |
| Number of Residues | 3 |
| Details | binding site for residue EDO D 409 |
| Chain | Residue |
| D | PRO301 |
| D | GLU313 |
| D | LYS316 |
| site_id | AH1 |
| Number of Residues | 4 |
| Details | binding site for residue EDO D 410 |
| Chain | Residue |
| D | GLY214 |
| D | HIS215 |
| D | EDO411 |
| S | PRO78 |
| site_id | AH2 |
| Number of Residues | 7 |
| Details | binding site for residue EDO D 411 |
| Chain | Residue |
| D | SER213 |
| D | TYR282 |
| D | ASN286 |
| D | PRO293 |
| D | EDO410 |
| D | HOH567 |
| S | PRO78 |
| site_id | AH3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO D 412 |
| Chain | Residue |
| D | GLU186 |
| D | ILE239 |
| D | GLU241 |
| site_id | AH4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO D 413 |
| Chain | Residue |
| D | MET295 |
| D | SER296 |
| S | ASN52 |
| S | GLY54 |
| S | ARG75 |
| site_id | AH5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO D 414 |
| Chain | Residue |
| A | VAL154 |
| A | PHE157 |
| D | ASP304 |
| D | ARG309 |
| D | HOH534 |
| site_id | AH6 |
| Number of Residues | 6 |
| Details | binding site for residue PEG D 415 |
| Chain | Residue |
| B | GLU132 |
| B | HIS133 |
| B | ARG136 |
| C | GLY36 |
| D | HIS121 |
| D | ALA123 |
| site_id | AH7 |
| Number of Residues | 11 |
| Details | binding site for residue PEG D 416 |
| Chain | Residue |
| D | GLY83 |
| D | VAL84 |
| D | CYS181 |
| D | VAL182 |
| D | ARG188 |
| D | GLY206 |
| D | VAL207 |
| D | ATP401 |
| D | HOH508 |
| D | HOH511 |
| D | HOH584 |
| site_id | AH8 |
| Number of Residues | 2 |
| Details | binding site for residue EDO R 101 |
| Chain | Residue |
| R | ASP53 |
| T | SER72 |
| site_id | AH9 |
| Number of Residues | 5 |
| Details | binding site for residue EDO T 101 |
| Chain | Residue |
| A | MET295 |
| A | SER296 |
| T | ASN52 |
| T | GLY54 |
| T | ARG75 |
Functional Information from PROSITE/UniProt
| site_id | PS00065 |
| Number of Residues | 29 |
| Details | D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. VAIVGvGGVGsvtaemltrcgigkLLlFD |
| Chain | Residue | Details |
| A | VAL76-ASP104 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1) => ECO:0000269|PubMed:25219498 |
| Chain | Residue | Details |
| Q | LYS69 | |
| B | CYS250 | |
| R | LYS69 | |
| D | CYS250 | |
| S | LYS69 | |
| T | LYS69 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20368332, ECO:0000269|PubMed:27653677, ECO:0000269|PubMed:30412706, ECO:0007744|PDB:5L95, ECO:0007744|PDB:6H77, ECO:0007744|PDB:6H78 |
| Chain | Residue | Details |
| A | GLY83 | |
| C | LYS127 | |
| C | ASN150 | |
| C | ASN184 | |
| D | GLY83 | |
| D | LYS127 | |
| D | ASN150 | |
| D | ASN184 | |
| A | LYS127 | |
| A | ASN150 | |
| A | ASN184 | |
| B | GLY83 | |
| B | LYS127 | |
| B | ASN150 | |
| B | ASN184 | |
| C | GLY83 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20368332, ECO:0000269|PubMed:27653677, ECO:0000269|PubMed:30412706, ECO:0007744|PDB:5L95, ECO:0007744|PDB:6H78 |
| Chain | Residue | Details |
| A | ASP104 | |
| B | ASP104 | |
| C | ASP104 | |
| D | ASP104 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20368332, ECO:0000269|PubMed:27653677, ECO:0000269|PubMed:30412706, ECO:0007744|PDB:5IAA, ECO:0007744|PDB:5L95, ECO:0007744|PDB:6H77, ECO:0007744|PDB:6H78 |
| Chain | Residue | Details |
| A | CYS226 | |
| C | CYS229 | |
| C | CYS303 | |
| C | CYS308 | |
| D | CYS226 | |
| D | CYS229 | |
| D | CYS303 | |
| D | CYS308 | |
| A | CYS229 | |
| A | CYS303 | |
| A | CYS308 | |
| B | CYS226 | |
| B | CYS229 | |
| B | CYS303 | |
| B | CYS308 | |
| C | CYS226 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER45 | |
| B | SER45 | |
| C | SER45 | |
| D | SER45 |
