6H6T

Binary crystal structure of positively and negatively supercharged variants Ftn(pos) and Ftn(neg) from human heavy chain ferritin (propandiol condition, coordination number 8)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006826	biological_process	iron ion transport
A	0006879	biological_process	intracellular iron ion homeostasis
A	0006880	biological_process	intracellular sequestering of iron ion
A	0006955	biological_process	immune response
A	0008043	cellular_component	intracellular ferritin complex
A	0008198	molecular_function	ferrous iron binding
A	0008199	molecular_function	ferric iron binding
A	0008285	biological_process	negative regulation of cell population proliferation
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0042802	molecular_function	identical protein binding
A	0044754	cellular_component	autolysosome
A	0046872	molecular_function	metal ion binding
A	0048147	biological_process	negative regulation of fibroblast proliferation
A	0070062	cellular_component	extracellular exosome
A	0110076	biological_process	negative regulation of ferroptosis
A	0140315	molecular_function	iron ion sequestering activity
A	1904724	cellular_component	tertiary granule lumen
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0004322	molecular_function	ferroxidase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006826	biological_process	iron ion transport
B	0006879	biological_process	intracellular iron ion homeostasis
B	0006880	biological_process	intracellular sequestering of iron ion
B	0006955	biological_process	immune response
B	0008043	cellular_component	intracellular ferritin complex
B	0008198	molecular_function	ferrous iron binding
B	0008199	molecular_function	ferric iron binding
B	0008285	biological_process	negative regulation of cell population proliferation
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0042802	molecular_function	identical protein binding
B	0044754	cellular_component	autolysosome
B	0046872	molecular_function	metal ion binding
B	0048147	biological_process	negative regulation of fibroblast proliferation
B	0070062	cellular_component	extracellular exosome
B	0110076	biological_process	negative regulation of ferroptosis
B	0140315	molecular_function	iron ion sequestering activity
B	1904724	cellular_component	tertiary granule lumen
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0004322	molecular_function	ferroxidase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006826	biological_process	iron ion transport
C	0006879	biological_process	intracellular iron ion homeostasis
C	0006880	biological_process	intracellular sequestering of iron ion
C	0006955	biological_process	immune response
C	0008043	cellular_component	intracellular ferritin complex
C	0008198	molecular_function	ferrous iron binding
C	0008199	molecular_function	ferric iron binding
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0042802	molecular_function	identical protein binding
C	0044754	cellular_component	autolysosome
C	0046872	molecular_function	metal ion binding
C	0048147	biological_process	negative regulation of fibroblast proliferation
C	0070062	cellular_component	extracellular exosome
C	0110076	biological_process	negative regulation of ferroptosis
C	0140315	molecular_function	iron ion sequestering activity
C	1904724	cellular_component	tertiary granule lumen
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0004322	molecular_function	ferroxidase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006826	biological_process	iron ion transport
D	0006879	biological_process	intracellular iron ion homeostasis
D	0006880	biological_process	intracellular sequestering of iron ion
D	0006955	biological_process	immune response
D	0008043	cellular_component	intracellular ferritin complex
D	0008198	molecular_function	ferrous iron binding
D	0008199	molecular_function	ferric iron binding
D	0008285	biological_process	negative regulation of cell population proliferation
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0042802	molecular_function	identical protein binding
D	0044754	cellular_component	autolysosome
D	0046872	molecular_function	metal ion binding
D	0048147	biological_process	negative regulation of fibroblast proliferation
D	0070062	cellular_component	extracellular exosome
D	0110076	biological_process	negative regulation of ferroptosis
D	0140315	molecular_function	iron ion sequestering activity
D	1904724	cellular_component	tertiary granule lumen
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0004322	molecular_function	ferroxidase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006826	biological_process	iron ion transport
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006880	biological_process	intracellular sequestering of iron ion
E	0006955	biological_process	immune response
E	0008043	cellular_component	intracellular ferritin complex
E	0008198	molecular_function	ferrous iron binding
E	0008199	molecular_function	ferric iron binding
E	0008285	biological_process	negative regulation of cell population proliferation
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0042802	molecular_function	identical protein binding
E	0044754	cellular_component	autolysosome
E	0046872	molecular_function	metal ion binding
E	0048147	biological_process	negative regulation of fibroblast proliferation
E	0070062	cellular_component	extracellular exosome
E	0110076	biological_process	negative regulation of ferroptosis
E	0140315	molecular_function	iron ion sequestering activity
E	1904724	cellular_component	tertiary granule lumen
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0004322	molecular_function	ferroxidase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006826	biological_process	iron ion transport
F	0006879	biological_process	intracellular iron ion homeostasis
F	0006880	biological_process	intracellular sequestering of iron ion
F	0006955	biological_process	immune response
F	0008043	cellular_component	intracellular ferritin complex
F	0008198	molecular_function	ferrous iron binding
F	0008199	molecular_function	ferric iron binding
F	0008285	biological_process	negative regulation of cell population proliferation
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0042802	molecular_function	identical protein binding
F	0044754	cellular_component	autolysosome
F	0046872	molecular_function	metal ion binding
F	0048147	biological_process	negative regulation of fibroblast proliferation
F	0070062	cellular_component	extracellular exosome
F	0110076	biological_process	negative regulation of ferroptosis
F	0140315	molecular_function	iron ion sequestering activity
F	1904724	cellular_component	tertiary granule lumen
F	1904813	cellular_component	ficolin-1-rich granule lumen
G	0004322	molecular_function	ferroxidase activity
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006826	biological_process	iron ion transport
G	0006879	biological_process	intracellular iron ion homeostasis
G	0006880	biological_process	intracellular sequestering of iron ion
G	0006955	biological_process	immune response
G	0008043	cellular_component	intracellular ferritin complex
G	0008198	molecular_function	ferrous iron binding
G	0008199	molecular_function	ferric iron binding
G	0008285	biological_process	negative regulation of cell population proliferation
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0042802	molecular_function	identical protein binding
G	0044754	cellular_component	autolysosome
G	0046872	molecular_function	metal ion binding
G	0048147	biological_process	negative regulation of fibroblast proliferation
G	0070062	cellular_component	extracellular exosome
G	0110076	biological_process	negative regulation of ferroptosis
G	0140315	molecular_function	iron ion sequestering activity
G	1904724	cellular_component	tertiary granule lumen
G	1904813	cellular_component	ficolin-1-rich granule lumen
H	0004322	molecular_function	ferroxidase activity
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006826	biological_process	iron ion transport
H	0006879	biological_process	intracellular iron ion homeostasis
H	0006880	biological_process	intracellular sequestering of iron ion
H	0006955	biological_process	immune response
H	0008043	cellular_component	intracellular ferritin complex
H	0008198	molecular_function	ferrous iron binding
H	0008199	molecular_function	ferric iron binding
H	0008285	biological_process	negative regulation of cell population proliferation
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0042802	molecular_function	identical protein binding
H	0044754	cellular_component	autolysosome
H	0046872	molecular_function	metal ion binding
H	0048147	biological_process	negative regulation of fibroblast proliferation
H	0070062	cellular_component	extracellular exosome
H	0110076	biological_process	negative regulation of ferroptosis
H	0140315	molecular_function	iron ion sequestering activity
H	1904724	cellular_component	tertiary granule lumen
H	1904813	cellular_component	ficolin-1-rich granule lumen
I	0004322	molecular_function	ferroxidase activity
I	0005506	molecular_function	iron ion binding
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006826	biological_process	iron ion transport
I	0006879	biological_process	intracellular iron ion homeostasis
I	0006880	biological_process	intracellular sequestering of iron ion
I	0006955	biological_process	immune response
I	0008043	cellular_component	intracellular ferritin complex
I	0008198	molecular_function	ferrous iron binding
I	0008199	molecular_function	ferric iron binding
I	0008285	biological_process	negative regulation of cell population proliferation
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0042802	molecular_function	identical protein binding
I	0044754	cellular_component	autolysosome
I	0046872	molecular_function	metal ion binding
I	0048147	biological_process	negative regulation of fibroblast proliferation
I	0070062	cellular_component	extracellular exosome
I	0110076	biological_process	negative regulation of ferroptosis
I	0140315	molecular_function	iron ion sequestering activity
I	1904724	cellular_component	tertiary granule lumen
I	1904813	cellular_component	ficolin-1-rich granule lumen
J	0004322	molecular_function	ferroxidase activity
J	0005506	molecular_function	iron ion binding
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006826	biological_process	iron ion transport
J	0006879	biological_process	intracellular iron ion homeostasis
J	0006880	biological_process	intracellular sequestering of iron ion
J	0006955	biological_process	immune response
J	0008043	cellular_component	intracellular ferritin complex
J	0008198	molecular_function	ferrous iron binding
J	0008199	molecular_function	ferric iron binding
J	0008285	biological_process	negative regulation of cell population proliferation
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0042802	molecular_function	identical protein binding
J	0044754	cellular_component	autolysosome
J	0046872	molecular_function	metal ion binding
J	0048147	biological_process	negative regulation of fibroblast proliferation
J	0070062	cellular_component	extracellular exosome
J	0110076	biological_process	negative regulation of ferroptosis
J	0140315	molecular_function	iron ion sequestering activity
J	1904724	cellular_component	tertiary granule lumen
J	1904813	cellular_component	ficolin-1-rich granule lumen
K	0004322	molecular_function	ferroxidase activity
K	0005506	molecular_function	iron ion binding
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006826	biological_process	iron ion transport
K	0006879	biological_process	intracellular iron ion homeostasis
K	0006880	biological_process	intracellular sequestering of iron ion
K	0006955	biological_process	immune response
K	0008043	cellular_component	intracellular ferritin complex
K	0008198	molecular_function	ferrous iron binding
K	0008199	molecular_function	ferric iron binding
K	0008285	biological_process	negative regulation of cell population proliferation
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0042802	molecular_function	identical protein binding
K	0044754	cellular_component	autolysosome
K	0046872	molecular_function	metal ion binding
K	0048147	biological_process	negative regulation of fibroblast proliferation
K	0070062	cellular_component	extracellular exosome
K	0110076	biological_process	negative regulation of ferroptosis
K	0140315	molecular_function	iron ion sequestering activity
K	1904724	cellular_component	tertiary granule lumen
K	1904813	cellular_component	ficolin-1-rich granule lumen
L	0004322	molecular_function	ferroxidase activity
L	0005506	molecular_function	iron ion binding
L	0005515	molecular_function	protein binding
L	0005576	cellular_component	extracellular region
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006826	biological_process	iron ion transport
L	0006879	biological_process	intracellular iron ion homeostasis
L	0006880	biological_process	intracellular sequestering of iron ion
L	0006955	biological_process	immune response
L	0008043	cellular_component	intracellular ferritin complex
L	0008198	molecular_function	ferrous iron binding
L	0008199	molecular_function	ferric iron binding
L	0008285	biological_process	negative regulation of cell population proliferation
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0042802	molecular_function	identical protein binding
L	0044754	cellular_component	autolysosome
L	0046872	molecular_function	metal ion binding
L	0048147	biological_process	negative regulation of fibroblast proliferation
L	0070062	cellular_component	extracellular exosome
L	0110076	biological_process	negative regulation of ferroptosis
L	0140315	molecular_function	iron ion sequestering activity
L	1904724	cellular_component	tertiary granule lumen
L	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue FE A 201

Chain	Residue
A	GLU27
A	GLU62
A	HIS65
A	GLN141

---

site_id	AC2
Number of Residues	3
Details	binding site for residue ACT A 202

Chain	Residue
A	ARG25
A	ASP84
A	GLN86

---

site_id	AC3
Number of Residues	5
Details	binding site for residue MG A 203

Chain	Residue
D	GLU134
F	ASP131
F	GLU134
A	ASP131
A	GLU134

---

site_id	AC4
Number of Residues	4
Details	binding site for residue FE B 201

Chain	Residue
B	GLU27
B	GLU62
B	HIS65
B	GLN141

---

site_id	AC5
Number of Residues	5
Details	binding site for residue MG B 202

Chain	Residue
B	ASP131
B	GLU134
C	GLU134
E	ASP131
E	GLU134

---

site_id	AC6
Number of Residues	5
Details	binding site for residue ZN C 201

Chain	Residue
A	HIS173
B	HIS173
C	HIS173
C	CL203
D	HIS173

---

site_id	AC7
Number of Residues	4
Details	binding site for residue FE C 202

Chain	Residue
C	GLU27
C	GLU62
C	HIS65
C	GLN141

---

site_id	AC8
Number of Residues	5
Details	binding site for residue CL C 203

Chain	Residue
A	HIS173
B	HIS173
C	HIS173
C	ZN201
D	HIS173

---

site_id	AC9
Number of Residues	4
Details	binding site for residue FE D 201

Chain	Residue
D	GLU27
D	GLU62
D	HIS65
D	GLN141

---

site_id	AD1
Number of Residues	8
Details	binding site for residue ZN E 201

Chain	Residue
E	HIS173
E	HIS173
E	HIS173
E	HIS173
E	CL203
E	CL203
E	CL203
E	CL203

---

site_id	AD2
Number of Residues	4
Details	binding site for residue FE E 202

Chain	Residue
E	GLU27
E	GLU62
E	HIS65
E	GLN141

---

site_id	AD3
Number of Residues	8
Details	binding site for residue CL E 203

Chain	Residue
E	HIS173
E	HIS173
E	HIS173
E	HIS173
E	ZN201
E	ZN201
E	ZN201
E	ZN201

---

site_id	AD4
Number of Residues	8
Details	binding site for residue ZN F 201

Chain	Residue
F	HIS173
F	HIS173
F	HIS173
F	HIS173
F	CL203
F	CL203
F	CL203
F	CL203

---

site_id	AD5
Number of Residues	3
Details	binding site for residue FE F 202

Chain	Residue
F	GLU27
F	GLU62
F	HIS65

---

site_id	AD6
Number of Residues	8
Details	binding site for residue CL F 203

Chain	Residue
F	HIS173
F	HIS173
F	HIS173
F	HIS173
F	ZN201
F	ZN201
F	ZN201
F	ZN201

---

site_id	AD7
Number of Residues	4
Details	binding site for residue FE G 201

Chain	Residue
G	GLU27
G	GLU62
G	HIS65
G	GLN141

---

site_id	AD8
Number of Residues	4
Details	binding site for residue ACT G 202

Chain	Residue
E	HIS13
G	ASP89
G	GLU102
G	GLU105

---

site_id	AD9
Number of Residues	5
Details	binding site for residue CL G 203

Chain	Residue
G	HIS173
H	HIS173
I	HIS173
K	HIS173
K	ZN201

---

site_id	AE1
Number of Residues	4
Details	binding site for residue FE H 201

Chain	Residue
H	GLU27
H	GLU62
H	HIS65
H	GLN141

---

site_id	AE2
Number of Residues	4
Details	binding site for residue FE I 201

Chain	Residue
I	GLU27
I	GLU62
I	HIS65
I	GLN141

---

site_id	AE3
Number of Residues	8
Details	binding site for residue ZN J 201

Chain	Residue
J	HIS173
J	HIS173
J	HIS173
J	HIS173
J	CL203
J	CL203
J	CL203
J	CL203

---

site_id	AE4
Number of Residues	4
Details	binding site for residue FE J 202

Chain	Residue
J	GLU27
J	GLU62
J	HIS65
J	GLN141

---

site_id	AE5
Number of Residues	8
Details	binding site for residue CL J 203

Chain	Residue
J	HIS173
J	HIS173
J	HIS173
J	HIS173
J	ZN201
J	ZN201
J	ZN201
J	ZN201

---

site_id	AE6
Number of Residues	6
Details	binding site for residue ZN K 201

Chain	Residue
G	HIS173
G	CL203
H	HIS173
I	HIS173
K	HIS173
K	HOH329

---

site_id	AE7
Number of Residues	4
Details	binding site for residue FE K 202

Chain	Residue
K	GLU27
K	GLU62
K	HIS65
K	GLN141

---

site_id	AE8
Number of Residues	8
Details	binding site for residue ZN L 201

Chain	Residue
L	HIS173
L	HIS173
L	HIS173
L	HIS173
L	CL203
L	CL203
L	CL203
L	CL203

---

site_id	AE9
Number of Residues	4
Details	binding site for residue FE L 202

Chain	Residue
L	GLU27
L	GLU62
L	HIS65
L	GLN141

---

site_id	AF1
Number of Residues	9
Details	binding site for residue CL L 203

Chain	Residue
L	LEU169
L	HIS173
L	HIS173
L	HIS173
L	HIS173
L	ZN201
L	ZN201
L	ZN201
L	ZN201

---

Functional Information from PROSITE/UniProt

site_id	PS00204
Number of Residues	21
Details	FERRITIN_2 Ferritin iron-binding regions signature 2. DphLCDFIEthYLneqvkaIK

Chain	Residue	Details
A	ASP126-LYS146
G	ASP126-LYS146

---

site_id	PS00540
Number of Residues	19
Details	FERRITIN_1 Ferritin iron-binding regions signature 1. EeREhaEKLMklQNqRgGR

Chain	Residue	Details
A	GLU61-ARG79
G	GLU61-ARG79

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:1992356, ECO:0007744|PDB:1FHA

Chain	Residue	Details
G	GLU27
K	HIS65
L	GLU27
L	HIS65
G	HIS65
H	GLU27
H	HIS65
I	GLU27
I	HIS65
J	GLU27
J	HIS65
K	GLU27

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00085

Chain	Residue	Details
G	GLU62
J	GLU62
J	GLU107
J	GLN141
K	GLU62
K	GLU107
K	GLN141
L	GLU62
L	GLU107
L	GLN141
G	GLU107
G	GLN141
H	GLU62
H	GLU107
H	GLN141
I	GLU62
I	GLU107
I	GLN141

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
G	MET0
H	MET0
I	MET0
J	MET0
K	MET0
L	MET0

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N-acetylthreonine; in Ferritin heavy chain, N-terminally processed => ECO:0007744|PubMed:22814378

Chain	Residue	Details
G	THR1
H	THR1
I	THR1
J	THR1
K	THR1
L	THR1

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18318008, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569

Chain	Residue	Details
G	SER178
H	SER178
I	SER178
J	SER178
K	SER178
L	SER178

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18318008

Chain	Residue	Details
G	SER182
H	SER182
I	SER182
J	SER182
K	SER182
L	SER182

---