6H6T
Binary crystal structure of positively and negatively supercharged variants Ftn(pos) and Ftn(neg) from human heavy chain ferritin (propandiol condition, coordination number 8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 4 |
| Unit cell lengths | 153.229, 153.229, 135.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.460 - 1.900 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cei |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.827 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | |
| Rmeas | 0.084 | |
| Number of reflections | 244878 | |
| <I/σ(I)> | 14.75 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 mM sodium acetate pH 4.5, 42 % propanediol |
