6H45
crystal structure of the human TGT catalytic subunit QTRT1 in complex with queuine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002099 | biological_process | tRNA wobble guanine modification |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005741 | cellular_component | mitochondrial outer membrane |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008033 | biological_process | tRNA processing |
| A | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0032473 | cellular_component | cytoplasmic side of mitochondrial outer membrane |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0120507 | cellular_component | tRNA-guanine transglycosylase complex |
| B | 0002099 | biological_process | tRNA wobble guanine modification |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005741 | cellular_component | mitochondrial outer membrane |
| B | 0006400 | biological_process | tRNA modification |
| B | 0008033 | biological_process | tRNA processing |
| B | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0032473 | cellular_component | cytoplasmic side of mitochondrial outer membrane |
| B | 0032991 | cellular_component | protein-containing complex |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0120507 | cellular_component | tRNA-guanine transglycosylase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue QEI A 501 |
| Chain | Residue |
| A | PHE109 |
| A | LEU230 |
| A | SER231 |
| A | GLY232 |
| A | MET259 |
| A | ASP159 |
| A | VAL161 |
| A | VAL162 |
| A | SER163 |
| A | SER164 |
| A | ILE200 |
| A | GLY228 |
| A | GLY229 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 502 |
| Chain | Residue |
| A | CYS317 |
| A | CYS319 |
| A | CYS322 |
| A | HIS348 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue BR A 503 |
| Chain | Residue |
| A | PHE310 |
| A | PHE310 |
| A | SER326 |
| A | SER326 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 504 |
| Chain | Residue |
| B | ARG327 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue K A 506 |
| Chain | Residue |
| A | TYR75 |
| A | LEU77 |
| A | ARG80 |
| A | GLY82 |
| A | GLU84 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | binding site for residue BR A 507 |
| Chain | Residue |
| A | CYS280 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | binding site for residue CL B 801 |
| Chain | Residue |
| A | ARG327 |
| B | VAL305 |
| site_id | AC8 |
| Number of Residues | 14 |
| Details | binding site for residue QEI B 802 |
| Chain | Residue |
| B | PHE109 |
| B | ASP159 |
| B | VAL161 |
| B | VAL162 |
| B | SER163 |
| B | SER164 |
| B | ILE200 |
| B | GLY228 |
| B | GLY229 |
| B | LEU230 |
| B | SER231 |
| B | GLY232 |
| B | MET259 |
| B | HOH929 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 803 |
| Chain | Residue |
| B | CYS317 |
| B | CYS319 |
| B | CYS322 |
| B | HIS348 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue BR B 804 |
| Chain | Residue |
| B | PHE310 |
| B | PHE310 |
| B | SER326 |
| B | SER326 |
| site_id | AD2 |
| Number of Residues | 2 |
| Details | binding site for residue GLU B 805 |
| Chain | Residue |
| B | GLU141 |
| B | ARG184 |
| site_id | AD3 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 807 |
| Chain | Residue |
| B | HOH928 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue K B 808 |
| Chain | Residue |
| B | TYR75 |
| B | LEU77 |
| B | ARG80 |
| B | GLY82 |
| B | GLU84 |
| site_id | AD5 |
| Number of Residues | 1 |
| Details | binding site for residue BR B 809 |
| Chain | Residue |
| B | CYS280 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | Region: {"description":"RNA binding","evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Region: {"description":"RNA binding; important for wobble base 34 recognition","evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"30149595","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34241577","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6H45","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7NQ4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03218","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"34241577","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7NQ4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
