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6FTG

Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes

This is a non-PDB format compatible entry.
Functional Information from GO Data
ChainGOidnamespacecontents
30006486biological_processprotein glycosylation
30008250cellular_componentoligosaccharyltransferase complex
50004576molecular_functionoligosaccharyl transferase activity
50004579molecular_functiondolichyl-diphosphooligosaccharide-protein glycotransferase activity
50005783cellular_componentendoplasmic reticulum
50005789cellular_componentendoplasmic reticulum membrane
50006486biological_processprotein glycosylation
50008250cellular_componentoligosaccharyltransferase complex
50016020cellular_componentmembrane
50016740molecular_functiontransferase activity
50016757molecular_functionglycosyltransferase activity
50018279biological_processprotein N-linked glycosylation via asparagine
50043686biological_processco-translational protein modification
50043687biological_processpost-translational protein modification
50046872molecular_functionmetal ion binding
A0002181biological_processcytoplasmic translation
A0003723molecular_functionRNA binding
A0003735molecular_functionstructural constituent of ribosome
A0005737cellular_componentcytoplasm
A0005840cellular_componentribosome
A0006412biological_processtranslation
A0015934cellular_componentlarge ribosomal subunit
A0019843molecular_functionrRNA binding
A0022625cellular_componentcytosolic large ribosomal subunit
A1990904cellular_componentribonucleoprotein complex
B0003723molecular_functionRNA binding
B0003735molecular_functionstructural constituent of ribosome
B0005634cellular_componentnucleus
B0005730cellular_componentnucleolus
B0005737cellular_componentcytoplasm
B0005840cellular_componentribosome
B0006412biological_processtranslation
B0022625cellular_componentcytosolic large ribosomal subunit
B1990904cellular_componentribonucleoprotein complex
C0003735molecular_functionstructural constituent of ribosome
C0005840cellular_componentribosome
C0006412biological_processtranslation
D0000027biological_processribosomal large subunit assembly
D0003723molecular_functionRNA binding
D0003735molecular_functionstructural constituent of ribosome
D0005634cellular_componentnucleus
D0005730cellular_componentnucleolus
D0005737cellular_componentcytoplasm
D0005840cellular_componentribosome
D0006412biological_processtranslation
D0008097molecular_function5S rRNA binding
D0019843molecular_functionrRNA binding
D0022625cellular_componentcytosolic large ribosomal subunit
D1990904cellular_componentribonucleoprotein complex
E0003735molecular_functionstructural constituent of ribosome
E0005840cellular_componentribosome
E0006412biological_processtranslation
F0000463biological_processmaturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
F0003723molecular_functionRNA binding
F0003735molecular_functionstructural constituent of ribosome
F0005737cellular_componentcytoplasm
F0005840cellular_componentribosome
F0022625cellular_componentcytosolic large ribosomal subunit
F1990904cellular_componentribonucleoprotein complex
G0003723molecular_functionRNA binding
G0042254biological_processribosome biogenesis
G1990904cellular_componentribonucleoprotein complex
H0002181biological_processcytoplasmic translation
H0003735molecular_functionstructural constituent of ribosome
H0005737cellular_componentcytoplasm
H0005840cellular_componentribosome
H0006412biological_processtranslation
H0019843molecular_functionrRNA binding
H0022625cellular_componentcytosolic large ribosomal subunit
H1990904cellular_componentribonucleoprotein complex
I0003735molecular_functionstructural constituent of ribosome
I0005737cellular_componentcytoplasm
I0005840cellular_componentribosome
I0006412biological_processtranslation
I0022625cellular_componentcytosolic large ribosomal subunit
I1990904cellular_componentribonucleoprotein complex
J0003723molecular_functionRNA binding
J0003735molecular_functionstructural constituent of ribosome
J0005634cellular_componentnucleus
J0005730cellular_componentnucleolus
J0005737cellular_componentcytoplasm
J0005840cellular_componentribosome
J0006412biological_processtranslation
J0019843molecular_functionrRNA binding
J1990904cellular_componentribonucleoprotein complex
L0003735molecular_functionstructural constituent of ribosome
L0005840cellular_componentribosome
L0006412biological_processtranslation
M0003723molecular_functionRNA binding
M0003735molecular_functionstructural constituent of ribosome
M0005840cellular_componentribosome
M0006412biological_processtranslation
N0002181biological_processcytoplasmic translation
N0003723molecular_functionRNA binding
N0003735molecular_functionstructural constituent of ribosome
N0005737cellular_componentcytoplasm
N0005840cellular_componentribosome
N0006412biological_processtranslation
N0022625cellular_componentcytosolic large ribosomal subunit
N0022626cellular_componentcytosolic ribosome
N0044391cellular_componentribosomal subunit
N1990904cellular_componentribonucleoprotein complex
O0003735molecular_functionstructural constituent of ribosome
O0005840cellular_componentribosome
O0006412biological_processtranslation
O0015934cellular_componentlarge ribosomal subunit
P0003735molecular_functionstructural constituent of ribosome
P0005840cellular_componentribosome
P0006412biological_processtranslation
P0015934cellular_componentlarge ribosomal subunit
Q0003735molecular_functionstructural constituent of ribosome
Q0005840cellular_componentribosome
Q0006412biological_processtranslation
R0003723molecular_functionRNA binding
R0003735molecular_functionstructural constituent of ribosome
R0005840cellular_componentribosome
R0006412biological_processtranslation
R0022625cellular_componentcytosolic large ribosomal subunit
S0003735molecular_functionstructural constituent of ribosome
S0005840cellular_componentribosome
S0006412biological_processtranslation
T0003735molecular_functionstructural constituent of ribosome
T0005737cellular_componentcytoplasm
T0005783cellular_componentendoplasmic reticulum
T0005829cellular_componentcytosol
T0005840cellular_componentribosome
T0006412biological_processtranslation
T0022625cellular_componentcytosolic large ribosomal subunit
T1990904cellular_componentribonucleoprotein complex
U0003735molecular_functionstructural constituent of ribosome
U0005840cellular_componentribosome
U0006412biological_processtranslation
V0003735molecular_functionstructural constituent of ribosome
V0005737cellular_componentcytoplasm
V0005840cellular_componentribosome
V0006412biological_processtranslation
V0015934cellular_componentlarge ribosomal subunit
V0022625cellular_componentcytosolic large ribosomal subunit
V0070180molecular_functionlarge ribosomal subunit rRNA binding
V1990904cellular_componentribonucleoprotein complex
W0003735molecular_functionstructural constituent of ribosome
X0003735molecular_functionstructural constituent of ribosome
X0005840cellular_componentribosome
X0006412biological_processtranslation
X0019843molecular_functionrRNA binding
Y0003723molecular_functionRNA binding
Y0003735molecular_functionstructural constituent of ribosome
Y0005737cellular_componentcytoplasm
Y0005840cellular_componentribosome
Y0006412biological_processtranslation
Y0015934cellular_componentlarge ribosomal subunit
Y1990904cellular_componentribonucleoprotein complex
Z0003735molecular_functionstructural constituent of ribosome
Z0005737cellular_componentcytoplasm
Z0005783cellular_componentendoplasmic reticulum
Z0005791cellular_componentrough endoplasmic reticulum
Z0005829cellular_componentcytosol
Z0005840cellular_componentribosome
Z0006412biological_processtranslation
Z0022625cellular_componentcytosolic large ribosomal subunit
Z0045202cellular_componentsynapse
Z1990904cellular_componentribonucleoprotein complex
a0003735molecular_functionstructural constituent of ribosome
a0005737cellular_componentcytoplasm
a0005840cellular_componentribosome
a0006412biological_processtranslation
a0015934cellular_componentlarge ribosomal subunit
a0022625cellular_componentcytosolic large ribosomal subunit
a1990904cellular_componentribonucleoprotein complex
b0003735molecular_functionstructural constituent of ribosome
b0005840cellular_componentribosome
b0006412biological_processtranslation
c0003723molecular_functionRNA binding
d0003735molecular_functionstructural constituent of ribosome
d0005840cellular_componentribosome
d0006412biological_processtranslation
e0003735molecular_functionstructural constituent of ribosome
e0005737cellular_componentcytoplasm
e0005840cellular_componentribosome
e0006412biological_processtranslation
e0022625cellular_componentcytosolic large ribosomal subunit
e1990904cellular_componentribonucleoprotein complex
f0000049molecular_functiontRNA binding
f0003723molecular_functionRNA binding
f0003735molecular_functionstructural constituent of ribosome
f0005737cellular_componentcytoplasm
f0005840cellular_componentribosome
f0006412biological_processtranslation
f0022625cellular_componentcytosolic large ribosomal subunit
f0045202cellular_componentsynapse
f1990904cellular_componentribonucleoprotein complex
g0003735molecular_functionstructural constituent of ribosome
g0005737cellular_componentcytoplasm
g0005783cellular_componentendoplasmic reticulum
g0005829cellular_componentcytosol
g0005840cellular_componentribosome
g0006412biological_processtranslation
g1990904cellular_componentribonucleoprotein complex
h0000463biological_processmaturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
h0003729molecular_functionmRNA binding
h0003735molecular_functionstructural constituent of ribosome
h0005737cellular_componentcytoplasm
h0005840cellular_componentribosome
h0006412biological_processtranslation
h0015934cellular_componentlarge ribosomal subunit
h0022625cellular_componentcytosolic large ribosomal subunit
h1990904cellular_componentribonucleoprotein complex
i0003735molecular_functionstructural constituent of ribosome
i0005737cellular_componentcytoplasm
i0005829cellular_componentcytosol
i0005840cellular_componentribosome
i0006412biological_processtranslation
i1990904cellular_componentribonucleoprotein complex
j0003735molecular_functionstructural constituent of ribosome
j0005840cellular_componentribosome
j0006412biological_processtranslation
k0003735molecular_functionstructural constituent of ribosome
k0005840cellular_componentribosome
k0006412biological_processtranslation
l0003735molecular_functionstructural constituent of ribosome
l0005737cellular_componentcytoplasm
l0005840cellular_componentribosome
l0006412biological_processtranslation
l0022625cellular_componentcytosolic large ribosomal subunit
l1990904cellular_componentribonucleoprotein complex
m0003735molecular_functionstructural constituent of ribosome
m0005840cellular_componentribosome
m0006412biological_processtranslation
n0003735molecular_functionstructural constituent of ribosome
n0005737cellular_componentcytoplasm
n0005840cellular_componentribosome
n0006412biological_processtranslation
n1990904cellular_componentribonucleoprotein complex
o0003735molecular_functionstructural constituent of ribosome
o0005840cellular_componentribosome
o0006412biological_processtranslation
p0003735molecular_functionstructural constituent of ribosome
p0005737cellular_componentcytoplasm
p0005840cellular_componentribosome
p0006412biological_processtranslation
p0008270molecular_functionzinc ion binding
p0022625cellular_componentcytosolic large ribosomal subunit
p0046872molecular_functionmetal ion binding
p0070180molecular_functionlarge ribosomal subunit rRNA binding
p1990904cellular_componentribonucleoprotein complex
r0003735molecular_functionstructural constituent of ribosome
r0005737cellular_componentcytoplasm
r0005840cellular_componentribosome
r0006412biological_processtranslation
r1990904cellular_componentribonucleoprotein complex
t0003723molecular_functionRNA binding
t0003735molecular_functionstructural constituent of ribosome
t0005737cellular_componentcytoplasm
t0005840cellular_componentribosome
t0006412biological_processtranslation
t0015934cellular_componentlarge ribosomal subunit
t0022625cellular_componentcytosolic large ribosomal subunit
t0070180molecular_functionlarge ribosomal subunit rRNA binding
t1990904cellular_componentribonucleoprotein complex
x0005048molecular_functionsignal sequence binding
x0005783cellular_componentendoplasmic reticulum
x0005784cellular_componentSec61 translocon complex
x0005789cellular_componentendoplasmic reticulum membrane
x0006613biological_processcotranslational protein targeting to membrane
x0006616biological_processSRP-dependent cotranslational protein targeting to membrane, translocation
x0008320molecular_functionprotein transmembrane transporter activity
x0015031biological_processprotein transport
x0016020cellular_componentmembrane
x0031204biological_processpost-translational protein targeting to membrane, translocation
x0039019biological_processpronephric nephron development
x0043022molecular_functionribosome binding
x0045047biological_processprotein targeting to ER
x0045048biological_processprotein insertion into ER membrane
y0005543molecular_functionphospholipid binding
y0005783cellular_componentendoplasmic reticulum
y0005789cellular_componentendoplasmic reticulum membrane
y0006605biological_processprotein targeting
y0006886biological_processintracellular protein transport
y0008320molecular_functionprotein transmembrane transporter activity
y0015031biological_processprotein transport
y0016020cellular_componentmembrane
y0022406biological_processmembrane docking
y0031204biological_processpost-translational protein targeting to membrane, translocation
y0043022molecular_functionribosome binding
y0045047biological_processprotein targeting to ER
y0071261cellular_componentSsh1 translocon complex
z0005784cellular_componentSec61 translocon complex
z0006886biological_processintracellular protein transport
Functional Information from PROSITE/UniProt
site_idPS00049
Number of Residues27
DetailsRIBOSOMAL_L14 Ribosomal protein L14 signature. AVVIrqrksyrrk.DGvflyFedNagVI
ChainResidueDetails
VALA79-ILE105

site_idPS00050
Number of Residues16
DetailsRIBOSOMAL_L23 Ribosomal protein L23 signature. KKAYVRLApdydaldV
ChainResidueDetails
XLYS134-VAL149

site_idPS00051
Number of Residues17
DetailsRIBOSOMAL_L39E Ribosomal protein L39e signature. KTgnkIryNskrRHWRR
ChainResidueDetails
lLYS30-ARG46

site_idPS00358
Number of Residues17
DetailsRIBOSOMAL_L5 Ribosomal protein L5 signature. LeqLTGQtpVfSkARyT
ChainResidueDetails
JLEU40-THR56

site_idPS00359
Number of Residues16
DetailsRIBOSOMAL_L11 Ribosomal protein L11 signature. KeIlGTaqSVGcNVdG
ChainResidueDetails
tLYS130-GLY145

site_idPS00464
Number of Residues25
DetailsRIBOSOMAL_L22 Ribosomal protein L22 signature. RrrTyRAhGRinpymsspCHIEMiL
ChainResidueDetails
PARG126-LEU150

site_idPS00467
Number of Residues12
DetailsRIBOSOMAL_L2 Ribosomal protein L2 signature. PrvRGVAmNPvE
ChainResidueDetails
APRO197-GLU208

site_idPS00474
Number of Residues24
DetailsRIBOSOMAL_L3 Ribosomal protein L3 signature. FgqdemiDviGvTkGKGykGvtsR
ChainResidueDetails
BPHE211-ARG234

site_idPS00475
Number of Residues32
DetailsRIBOSOMAL_L15 Ribosomal protein L15 signature. KVLGkGkLpkqp.ViVkakfFSrrAeekIkgvG
ChainResidueDetails
aLYS110-GLY141

site_idPS00526
Number of Residues20
DetailsRIBOSOMAL_L19E Ribosomal protein L19e signature. QKRLaSsvlrCGkkkVWLDP
ChainResidueDetails
RGLN7-PRO26

site_idPS00579
Number of Residues15
DetailsRIBOSOMAL_L29 Ribosomal protein L29 signature. KLSKIRvVRKSIARV
ChainResidueDetails
hLYS43-VAL57

site_idPS00580
Number of Residues21
DetailsRIBOSOMAL_L32E Ribosomal protein L32e signature. FiRhqsdRyvKIkrn..WRKprG
ChainResidueDetails
ePHE20-GLY40

site_idPS00634
Number of Residues33
DetailsRIBOSOMAL_L30 Ribosomal protein L30 signature. VLqlLrLrQifNgTfvklnkasinMLriVepYI
ChainResidueDetails
FVAL110-ILE142

site_idPS00687
Number of Residues8
DetailsALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. GESSGKNV
ChainResidueDetails
CGLY15-VAL22

site_idPS00700
Number of Residues22
DetailsRIBOSOMAL_L6_2 Ribosomal protein L6 signature 2. QattVknKdiRkFlDGIYVseK
ChainResidueDetails
HGLN163-LYS184

site_idPS00709
Number of Residues25
DetailsRIBOSOMAL_L30E_1 Ribosomal protein L30e signature 1. SgkyvlGyKqtLKmiRqGkaKlVIL
ChainResidueDetails
cSER24-LEU48

site_idPS00755
Number of Residues20
DetailsSECY_1 Protein secY signature 1. TLMeLGIsPIVtSGLIMQLL
ChainResidueDetails
xTHR75-LEU94

site_idPS00756
Number of Residues19
DetailsSECY_2 Protein secY signature 2. LLdElLQkgyGLGSGiSLF
ChainResidueDetails
xLEU164-PHE182

site_idPS00783
Number of Residues24
DetailsRIBOSOMAL_L13 Ribosomal protein L13 signature. VRGMLPhktkrGQaaldrLkVFdG
ChainResidueDetails
OVAL84-GLY107

site_idPS00939
Number of Residues27
DetailsRIBOSOMAL_L1E Ribosomal protein L1e signature. NttqKryAIcSALaASAlpalvmsKGH
ChainResidueDetails
CASN116-HIS142

site_idPS00993
Number of Residues21
DetailsRIBOSOMAL_L30E_2 Ribosomal protein L30e signature 2. ELGTacGKyyrvctLaIiDpG
ChainResidueDetails
cGLU80-GLY100

site_idPS01067
Number of Residues29
DetailsSECE_SEC61G Protein secE/sec61-gamma signature. FvKDsIrlVkRctKPdrkEfqkiaMATAI
ChainResidueDetails
yPHE14-ILE42

site_idPS01073
Number of Residues18
DetailsRIBOSOMAL_L24E Ribosomal protein L24e signature. FsGykIyPGhGrrYaRtD
ChainResidueDetails
WPHE8-ASP25

site_idPS01077
Number of Residues20
DetailsRIBOSOMAL_L37E Ribosomal protein L37e signature. GTsSfGkRrnkt.HtlCrRCG
ChainResidueDetails
jGLY4-GLY23

site_idPS01082
Number of Residues18
DetailsRIBOSOMAL_L7AE Ribosomal protein L7Ae signature. CrkmgVPYciLkGKarLG
ChainResidueDetails
GCYS174-GLY191

site_idPS01104
Number of Residues15
DetailsRIBOSOMAL_L13E Ribosomal protein L13e signature. RaGRGFSLeELrvaG
ChainResidueDetails
LARG71-GLY85

site_idPS01105
Number of Residues22
DetailsRIBOSOMAL_L35AE Ribosomal protein L35Ae signature. GKVtRaHGnsGmVrAkFrsnLP
ChainResidueDetails
fGLY72-PRO93

site_idPS01106
Number of Residues18
DetailsRIBOSOMAL_L18E Ribosomal protein L18e signature. KrLfmSRtnRPpLSLsRM
ChainResidueDetails
QLYS49-MET66

site_idPS01107
Number of Residues12
DetailsRIBOSOMAL_L27E Ribosomal protein L27e signature. GKNkWFFqkLRF
ChainResidueDetails
ZGLY125-PHE136

site_idPS01108
Number of Residues19
DetailsRIBOSOMAL_L24 Ribosomal protein L24 signature. DDeVqVVrGhyKGqqiGkV
ChainResidueDetails
YASP52-VAL70

site_idPS01144
Number of Residues15
DetailsRIBOSOMAL_L31E Ribosomal protein L31e signature. VRIDtrLNkAvWaKG
ChainResidueDetails
dVAL62-GLY76

site_idPS01145
Number of Residues14
DetailsRIBOSOMAL_L34E Ribosomal protein L34e signature. TaSnKtrlsRTPGN
ChainResidueDetails
gTHR15-ASN28

site_idPS01170
Number of Residues19
DetailsRIBOSOMAL_L6E Ribosomal protein L6e signature. NrvPLRRthqkFVIATSt.K
ChainResidueDetails
EASN178-LYS196

site_idPS01171
Number of Residues26
DetailsRIBOSOMAL_L21E Ribosomal protein L21e signature. GDiVdikgmgtvqk.GmpHkcYhGkTG
ChainResidueDetails
TGLY37-GLY62

site_idPS01172
Number of Residues12
DetailsRIBOSOMAL_L44E Ribosomal protein L44e signature. KtTKKivLRleC
ChainResidueDetails
oLYS61-CYS72

site_idPS01190
Number of Residues11
DetailsRIBOSOMAL_L36E Ribosomal protein L36e signature. PYERRaMELLK
ChainResidueDetails
iPRO52-LYS62

site_idPS01194
Number of Residues24
DetailsRIBOSOMAL_L15E Ribosomal protein L15e signature. DKARrLGYkAkqGYVIYRiRVrRG
ChainResidueDetails
NASP46-GLY69

site_idPS01257
Number of Residues22
DetailsRIBOSOMAL_L10E Ribosomal protein L10e signature. ADRlqtGMRgAFGKPqGtvARV
ChainResidueDetails
IALA108-VAL129

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues64
DetailsTOPO_DOM: Cytoplasmic => ECO:0000305
ChainResidueDetails
5MET1-ASP15
xGLY444-PHE458
5GLY142
5THR189-SER191
5GLY230-HIS234
5ASP284-LEU298
5SER380-ALA385
5TYR429-LYS453
xASP357-TRP379
xALA421-PHE437

site_idSWS_FT_FI2
Number of Residues165
DetailsTRANSMEM: Helical => ECO:0000269|PubMed:29519914
ChainResidueDetails
5THR16-ARG34
5ILE112-ALA141
5ALA143-ILE158
5ILE171-LYS188
5ILE192-SER207
5GLY211-THR229
5ARG235-GLN253
5GLU266-VAL283
5PHE299-LEU317

site_idSWS_FT_FI3
Number of Residues372
DetailsTOPO_DOM: Lumenal => ECO:0000305
ChainResidueDetails
5LEU35-ASP111
5SER159-GLY170
5SER208-GLY210
5ILE254-SER265
5MET318-THR356
5MET403-LEU406
5HIS474-THR705

site_idSWS_FT_FI4
Number of Residues78
DetailsTRANSMEM: Helical => ECO:0000250|UniProtKB:P39007
ChainResidueDetails
5TRP357-PHE379
5ARG386-VAL402
5MET407-THR428
5ASN454-PHE473

site_idSWS_FT_FI5
Number of Residues5
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:B9KDD4
ChainResidueDetails
5ASP49
5ASP167
5GLU169
5ARG405
5TYR530

site_idSWS_FT_FI6
Number of Residues3
DetailsSITE: Interacts with target acceptor peptide in protein substrate => ECO:0000250|UniProtKB:B9KDD4
ChainResidueDetails
5ASP49
5GLU351
5LYS595
BLYS226
BLYS386
BLYS393

site_idSWS_FT_FI7
Number of Residues1
DetailsSITE: Important for catalytic activity => ECO:0000250|UniProtKB:B9KDD4
ChainResidueDetails
5ARG160
BLYS366

site_idSWS_FT_FI8
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498
ChainResidueDetails
5ASN537
5ASN544

site_idSWS_FT_FI9
Number of Residues1
DetailsCARBOHYD: N-linked (GlcNAc...) (high mannose) asparagine => ECO:0000250|UniProtKB:P39007
ChainResidueDetails
5ASN548
CVAL313

site_idSWS_FT_FI10
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate => ECO:0000250|UniProtKB:P36578
ChainResidueDetails
CHIS347

237423

PDB entries from 2025-06-11

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