6FGA

Crystal structure of TRIM21 E3 ligase, RING domain in complex with its cognate E2 conjugating enzyme UBE2E1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0016567	biological_process	protein ubiquitination
A	0046872	molecular_function	metal ion binding
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0016567	biological_process	protein ubiquitination
B	0046872	molecular_function	metal ion binding
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0016567	biological_process	protein ubiquitination
C	0046872	molecular_function	metal ion binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0016567	biological_process	protein ubiquitination
D	0046872	molecular_function	metal ion binding
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0016567	biological_process	protein ubiquitination
E	0046872	molecular_function	metal ion binding
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0016567	biological_process	protein ubiquitination
F	0046872	molecular_function	metal ion binding
G	0004842	molecular_function	ubiquitin-protein transferase activity
G	0016567	biological_process	protein ubiquitination
G	0046872	molecular_function	metal ion binding
H	0004842	molecular_function	ubiquitin-protein transferase activity
H	0016567	biological_process	protein ubiquitination
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 101

Chain	Residue
A	CYS31
A	HIS33
A	CYS51
A	CYS54

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 102

Chain	Residue
A	CYS16
A	CYS19
A	CYS36
A	CYS39

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN B 101

Chain	Residue
B	HIS33
B	CYS51
B	CYS54
B	CYS31

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN B 102

Chain	Residue
B	CYS16
B	CYS19
B	CYS36
B	CYS39

---

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN C 101

Chain	Residue
C	CYS31
C	HIS33
C	CYS51
C	CYS54

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN C 102

Chain	Residue
C	CYS16
C	CYS19
C	CYS36
C	CYS39

---

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN D 101

Chain	Residue
D	CYS31
D	HIS33
D	CYS51
D	CYS54

---

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN D 102

Chain	Residue
D	CYS16
D	CYS19
D	CYS36
D	CYS39

---

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN E 101

Chain	Residue
E	CYS31
E	HIS33
E	CYS51
E	CYS54

---

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN E 102

Chain	Residue
E	CYS16
E	CYS19
E	CYS36
E	CYS39

---

site_id	AD2
Number of Residues	4
Details	binding site for residue ZN F 101

Chain	Residue
F	CYS31
F	HIS33
F	CYS51
F	CYS54

---

site_id	AD3
Number of Residues	4
Details	binding site for residue ZN F 102

Chain	Residue
F	CYS16
F	CYS19
F	CYS36
F	CYS39

---

site_id	AD4
Number of Residues	4
Details	binding site for residue ZN G 101

Chain	Residue
G	CYS31
G	HIS33
G	CYS51
G	CYS54

---

site_id	AD5
Number of Residues	4
Details	binding site for residue ZN G 102

Chain	Residue
G	CYS16
G	CYS19
G	CYS36
G	CYS39

---

site_id	AD6
Number of Residues	4
Details	binding site for residue ZN H 101

Chain	Residue
H	CYS31
H	HIS33
H	CYS51
H	CYS54

---

site_id	AD7
Number of Residues	4
Details	binding site for residue ZN H 102

Chain	Residue
H	CYS16
H	CYS19
H	CYS36
H	CYS39

---

site_id	AD8
Number of Residues	6
Details	binding site for residue GOL I 201

Chain	Residue
I	PRO111
I	LYS112
I	VAL113
I	THR114
I	VAL129
I	ILE130

---

site_id	AD9
Number of Residues	7
Details	binding site for residue GOL I 202

Chain	Residue
I	PHE115
I	ARG116
I	THR117
I	ASN125
I	SER126
I	GLY128
I	HOH303

---

site_id	AE1
Number of Residues	9
Details	binding site for residue GOL J 201

Chain	Residue
J	PRO111
J	LYS112
J	VAL113
J	THR114
J	VAL129
J	ILE130
J	LEU135
J	LYS136
J	HOH303

---

site_id	AE2
Number of Residues	7
Details	binding site for residue GOL N 201

Chain	Residue
N	PRO111
N	LYS112
N	VAL113
N	THR114
N	VAL129
N	ILE130
N	LEU135

---

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHCNInsq.GvICLdiL

Chain	Residue	Details
I	TYR120-LEU135

---

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CgHsFCqeCI

Chain	Residue	Details
A	CYS31-ILE40

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	7
Details	ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133

Chain	Residue	Details
I	CYS131
J	CYS131
K	CYS131
L	CYS131
M	CYS131
N	CYS131
O	CYS131
H	CYS16-ARG55

---

site_id	SWS_FT_FI2
Number of Residues	14
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)

Chain	Residue	Details
I	LYS136
E	HIS95
N	LYS136
F	HIS95
O	LYS136
G	HIS95
H	CYS92
H	HIS95
A	HIS95
J	LYS136
B	HIS95
K	LYS136
C	HIS95
L	LYS136
D	HIS95
M	LYS136

---