6F6Z
Mouse Thymidylate Synthase Cocrystallized with N(4)OHdCMP and Soaked in Methylenetetrahydrofolate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0032259 | biological_process | methylation |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005743 | cellular_component | mitochondrial inner membrane |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0032259 | biological_process | methylation |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 19 |
| Details | binding site for residue NOH A 501 |
| Chain | Residue |
| A | ARG44 |
| A | ASP212 |
| A | ASN220 |
| A | HIS250 |
| A | TYR252 |
| A | TGQ502 |
| A | HOH603 |
| A | HOH604 |
| A | HOH618 |
| B | ARG169 |
| B | ARG170 |
| A | TRP103 |
| A | LEU186 |
| A | CYS189 |
| A | HIS190 |
| A | GLN208 |
| A | ARG209 |
| A | SER210 |
| A | GLY211 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | binding site for residue TGQ A 502 |
| Chain | Residue |
| A | PHE74 |
| A | ILE102 |
| A | ASP212 |
| A | LEU215 |
| A | GLY216 |
| A | PHE219 |
| A | ASN220 |
| A | MET305 |
| A | ALA306 |
| A | NOH501 |
| site_id | AC3 |
| Number of Residues | 17 |
| Details | binding site for residue NOH B 501 |
| Chain | Residue |
| A | ARG169 |
| A | ARG170 |
| B | ARG44 |
| B | TRP103 |
| B | CYS189 |
| B | HIS190 |
| B | GLN208 |
| B | ARG209 |
| B | SER210 |
| B | GLY211 |
| B | ASP212 |
| B | ASN220 |
| B | HIS250 |
| B | TYR252 |
| B | TGQ502 |
| B | HOH609 |
| B | HOH613 |
| site_id | AC4 |
| Number of Residues | 14 |
| Details | binding site for residue TGQ B 502 |
| Chain | Residue |
| B | PHE74 |
| B | ILE102 |
| B | TRP103 |
| B | ASN106 |
| B | ASP212 |
| B | LEU215 |
| B | GLY216 |
| B | PHE219 |
| B | ASN220 |
| B | MET305 |
| B | ALA306 |
| B | NOH501 |
| B | HOH619 |
| B | HOH654 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNpkdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| A | ARG169-VAL197 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | CYS189 | |
| B | CYS189 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | ARG44 | |
| A | ASN220 | |
| B | ARG44 | |
| B | ASN220 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| A | ARG169 | |
| B | ARG169 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| A | CYS189 | |
| A | ARG209 | |
| A | HIS250 | |
| B | CYS189 | |
| B | ARG209 | |
| B | HIS250 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | ASP212 | |
| A | ALA306 | |
| B | ASP212 | |
| B | ALA306 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P04818 |
| Chain | Residue | Details |
| A | SER108 | |
| B | SER108 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P04818 |
| Chain | Residue | Details |
| A | LYS286 | |
| A | LYS302 | |
| B | LYS286 | |
| B | LYS302 |
