6F0X

Cryo-EM structure of TRIP13 in complex with ATP gamma S, p31comet, C-Mad2 and Cdc20

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001556	biological_process	oocyte maturation
A	0001673	cellular_component	male germ cell nucleus
A	0003712	molecular_function	transcription coregulator activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0006302	biological_process	double-strand break repair
A	0006366	biological_process	transcription by RNA polymerase II
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0007130	biological_process	synaptonemal complex assembly
A	0007131	biological_process	reciprocal meiotic recombination
A	0007141	biological_process	male meiosis I
A	0007144	biological_process	female meiosis I
A	0007283	biological_process	spermatogenesis
A	0007286	biological_process	spermatid development
A	0016887	molecular_function	ATP hydrolysis activity
A	0030154	biological_process	cell differentiation
A	0042802	molecular_function	identical protein binding
A	0048477	biological_process	oogenesis
A	0051321	biological_process	meiotic cell cycle
A	0051598	biological_process	meiotic recombination checkpoint signaling
B	0000166	molecular_function	nucleotide binding
B	0001556	biological_process	oocyte maturation
B	0001673	cellular_component	male germ cell nucleus
B	0003712	molecular_function	transcription coregulator activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0006302	biological_process	double-strand break repair
B	0006366	biological_process	transcription by RNA polymerase II
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0007130	biological_process	synaptonemal complex assembly
B	0007131	biological_process	reciprocal meiotic recombination
B	0007141	biological_process	male meiosis I
B	0007144	biological_process	female meiosis I
B	0007283	biological_process	spermatogenesis
B	0007286	biological_process	spermatid development
B	0016887	molecular_function	ATP hydrolysis activity
B	0030154	biological_process	cell differentiation
B	0042802	molecular_function	identical protein binding
B	0048477	biological_process	oogenesis
B	0051321	biological_process	meiotic cell cycle
B	0051598	biological_process	meiotic recombination checkpoint signaling
C	0000166	molecular_function	nucleotide binding
C	0001556	biological_process	oocyte maturation
C	0001673	cellular_component	male germ cell nucleus
C	0003712	molecular_function	transcription coregulator activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0006302	biological_process	double-strand break repair
C	0006366	biological_process	transcription by RNA polymerase II
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0007130	biological_process	synaptonemal complex assembly
C	0007131	biological_process	reciprocal meiotic recombination
C	0007141	biological_process	male meiosis I
C	0007144	biological_process	female meiosis I
C	0007283	biological_process	spermatogenesis
C	0007286	biological_process	spermatid development
C	0016887	molecular_function	ATP hydrolysis activity
C	0030154	biological_process	cell differentiation
C	0042802	molecular_function	identical protein binding
C	0048477	biological_process	oogenesis
C	0051321	biological_process	meiotic cell cycle
C	0051598	biological_process	meiotic recombination checkpoint signaling
D	0000166	molecular_function	nucleotide binding
D	0001556	biological_process	oocyte maturation
D	0001673	cellular_component	male germ cell nucleus
D	0003712	molecular_function	transcription coregulator activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0006302	biological_process	double-strand break repair
D	0006366	biological_process	transcription by RNA polymerase II
D	0007094	biological_process	mitotic spindle assembly checkpoint signaling
D	0007130	biological_process	synaptonemal complex assembly
D	0007131	biological_process	reciprocal meiotic recombination
D	0007141	biological_process	male meiosis I
D	0007144	biological_process	female meiosis I
D	0007283	biological_process	spermatogenesis
D	0007286	biological_process	spermatid development
D	0016887	molecular_function	ATP hydrolysis activity
D	0030154	biological_process	cell differentiation
D	0042802	molecular_function	identical protein binding
D	0048477	biological_process	oogenesis
D	0051321	biological_process	meiotic cell cycle
D	0051598	biological_process	meiotic recombination checkpoint signaling
E	0000166	molecular_function	nucleotide binding
E	0001556	biological_process	oocyte maturation
E	0001673	cellular_component	male germ cell nucleus
E	0003712	molecular_function	transcription coregulator activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005694	cellular_component	chromosome
E	0006302	biological_process	double-strand break repair
E	0006366	biological_process	transcription by RNA polymerase II
E	0007094	biological_process	mitotic spindle assembly checkpoint signaling
E	0007130	biological_process	synaptonemal complex assembly
E	0007131	biological_process	reciprocal meiotic recombination
E	0007141	biological_process	male meiosis I
E	0007144	biological_process	female meiosis I
E	0007283	biological_process	spermatogenesis
E	0007286	biological_process	spermatid development
E	0016887	molecular_function	ATP hydrolysis activity
E	0030154	biological_process	cell differentiation
E	0042802	molecular_function	identical protein binding
E	0048477	biological_process	oogenesis
E	0051321	biological_process	meiotic cell cycle
E	0051598	biological_process	meiotic recombination checkpoint signaling
F	0000166	molecular_function	nucleotide binding
F	0001556	biological_process	oocyte maturation
F	0001673	cellular_component	male germ cell nucleus
F	0003712	molecular_function	transcription coregulator activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005694	cellular_component	chromosome
F	0006302	biological_process	double-strand break repair
F	0006366	biological_process	transcription by RNA polymerase II
F	0007094	biological_process	mitotic spindle assembly checkpoint signaling
F	0007130	biological_process	synaptonemal complex assembly
F	0007131	biological_process	reciprocal meiotic recombination
F	0007141	biological_process	male meiosis I
F	0007144	biological_process	female meiosis I
F	0007283	biological_process	spermatogenesis
F	0007286	biological_process	spermatid development
F	0016887	molecular_function	ATP hydrolysis activity
F	0030154	biological_process	cell differentiation
F	0042802	molecular_function	identical protein binding
F	0048477	biological_process	oogenesis
F	0051321	biological_process	meiotic cell cycle
F	0051598	biological_process	meiotic recombination checkpoint signaling
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005730	cellular_component	nucleolus
P	0005737	cellular_component	cytoplasm
P	0005819	cellular_component	spindle
P	0007096	biological_process	regulation of exit from mitosis
P	0031965	cellular_component	nuclear membrane
P	1902426	biological_process	deactivation of mitotic spindle assembly checkpoint
Q	0000776	cellular_component	kinetochore
Q	0000922	cellular_component	spindle pole
Q	0005515	molecular_function	protein binding
Q	0005654	cellular_component	nucleoplasm
Q	0005680	cellular_component	anaphase-promoting complex
Q	0005813	cellular_component	centrosome
Q	0005819	cellular_component	spindle
Q	0005829	cellular_component	cytosol
Q	0007064	biological_process	mitotic sister chromatid cohesion
Q	0007094	biological_process	mitotic spindle assembly checkpoint signaling
Q	0007346	biological_process	regulation of mitotic cell cycle
Q	0007399	biological_process	nervous system development
Q	0008284	biological_process	positive regulation of cell population proliferation
Q	0010997	molecular_function	anaphase-promoting complex binding
Q	0016567	biological_process	protein ubiquitination
Q	0030154	biological_process	cell differentiation
Q	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
Q	0031915	biological_process	positive regulation of synaptic plasticity
Q	0032991	cellular_component	protein-containing complex
Q	0033597	cellular_component	mitotic checkpoint complex
Q	0040020	biological_process	regulation of meiotic nuclear division
Q	0042826	molecular_function	histone deacetylase binding
Q	0044784	biological_process	metaphase/anaphase transition of cell cycle
Q	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
Q	0048471	cellular_component	perinuclear region of cytoplasm
Q	0050773	biological_process	regulation of dendrite development
Q	0051301	biological_process	cell division
Q	0051445	biological_process	regulation of meiotic cell cycle
Q	0090129	biological_process	positive regulation of synapse maturation
Q	0090307	biological_process	mitotic spindle assembly
Q	0097027	molecular_function	ubiquitin-protein transferase activator activity
Q	1904668	biological_process	positive regulation of ubiquitin protein ligase activity
Q	1905786	biological_process	positive regulation of anaphase-promoting complex-dependent catabolic process
Q	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
Q	1990757	molecular_function	ubiquitin ligase activator activity
Q	1990949	biological_process	metaphase/anaphase transition of meiosis I
Z	0000070	biological_process	mitotic sister chromatid segregation
Z	0000775	cellular_component	chromosome, centromeric region
Z	0000776	cellular_component	kinetochore
Z	0000922	cellular_component	spindle pole
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005654	cellular_component	nucleoplasm
Z	0005694	cellular_component	chromosome
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0007094	biological_process	mitotic spindle assembly checkpoint signaling
Z	0033597	cellular_component	mitotic checkpoint complex
Z	0042177	biological_process	negative regulation of protein catabolic process
Z	0042802	molecular_function	identical protein binding
Z	0042803	molecular_function	protein homodimerization activity
Z	0044615	cellular_component	nuclear pore nuclear basket
Z	0045930	biological_process	negative regulation of mitotic cell cycle
Z	0048471	cellular_component	perinuclear region of cytoplasm
Z	0051301	biological_process	cell division
Z	0051783	biological_process	regulation of nuclear division
Z	0072686	cellular_component	mitotic spindle
Z	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
Z	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
Z	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
Z	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue AGS A 901

Chain	Residue
A	SER138
A	SER187
A	ASN300
A	ILE330
A	GLY385
A	ARG386
A	ARG389
B	ASP285
B	ARG312
A	LEU139
A	VAL140
A	TYR141
A	GLY182
A	THR183
A	GLY184
A	LYS185
A	THR186

---

site_id	AC2
Number of Residues	13
Details	binding site for residue AGS B 901

Chain	Residue
B	SER138
B	LEU139
B	VAL140
B	TYR141
B	THR183
B	GLY184
B	LYS185
B	THR186
B	SER187
B	ASN300
B	ARG386
B	ARG389
C	ARG312

---

site_id	AC3
Number of Residues	17
Details	binding site for residue AGS C 901

Chain	Residue
C	SER138
C	LEU139
C	TYR141
C	PRO181
C	GLY182
C	THR183
C	GLY184
C	LYS185
C	THR186
C	SER187
C	LEU188
C	ASN300
C	PRO322
C	GLY385
C	ARG386
C	ARG389
D	ASP311

---

site_id	AC4
Number of Residues	13
Details	binding site for residue AGS D 901

Chain	Residue
D	SER138
D	LEU139
D	VAL140
D	GLY182
D	THR183
D	GLY184
D	LYS185
D	THR186
D	SER187
D	LEU188
D	ILE330
D	ARG386
D	ARG389

---

site_id	AC5
Number of Residues	17
Details	binding site for Di-peptide AGS E 901 and ARG E 386

Chain	Residue
E	LEU139
E	VAL140
E	TYR141
E	GLY182
E	THR183
E	GLY184
E	LYS185
E	THR186
E	SER187
E	ASP252
E	ILE330
E	SER384
E	GLY385
E	VAL387
E	LEU388
E	ARG389
E	LYS390

---

site_id	AC6
Number of Residues	18
Details	binding site for Di-peptide AGS E 901 and LYS E 185

Chain	Residue
E	LEU139
E	VAL140
E	TYR141
E	PRO180
E	PRO181
E	GLY182
E	THR183
E	GLY184
E	THR186
E	SER187
E	LEU188
E	CYS189
E	ASP252
E	THR298
E	ILE330
E	GLY385
E	ARG386
E	ARG389

---

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. TGGGtsDrHIRIWNV

Chain	Residue	Details
Q	THR373-VAL387

---

site_id	PS00674
Number of Residues	20
Details	AAA AAA-protein family signature. VvILtTSNitekIDvAFvdR

Chain	Residue	Details
A	VAL293-ARG312

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	42
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	183
Details	Domain: {"description":"HORMA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00109","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Region: {"description":"Required for assuming the closed conformation and for interaction with CDC20"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Dhillon A.S.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---