6EBK

The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002244	biological_process	hematopoietic progenitor cell differentiation
A	0004033	molecular_function	aldo-keto reductase (NADPH) activity
A	0005249	molecular_function	voltage-gated potassium channel activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005886	cellular_component	plasma membrane
A	0006813	biological_process	potassium ion transport
A	0008076	cellular_component	voltage-gated potassium channel complex
A	0009898	cellular_component	cytoplasmic side of plasma membrane
A	0014069	cellular_component	postsynaptic density
A	0015459	molecular_function	potassium channel regulator activity
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0030424	cellular_component	axon
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0034702	cellular_component	monoatomic ion channel complex
A	0034705	cellular_component	potassium channel complex
A	0043005	cellular_component	neuron projection
A	0043194	cellular_component	axon initial segment
A	0043679	cellular_component	axon terminus
A	0044224	cellular_component	juxtaparanode region of axon
A	0044325	molecular_function	transmembrane transporter binding
A	0044877	molecular_function	protein-containing complex binding
A	0045202	cellular_component	synapse
A	0045445	biological_process	myoblast differentiation
A	0050905	biological_process	neuromuscular process
A	0055085	biological_process	transmembrane transport
A	0055114	biological_process	obsolete oxidation-reduction process
A	0070995	biological_process	NADPH oxidation
A	0071805	biological_process	potassium ion transmembrane transport
A	0098839	cellular_component	postsynaptic density membrane
A	0098900	biological_process	regulation of action potential
A	0098978	cellular_component	glutamatergic synapse
A	1901379	biological_process	regulation of potassium ion transmembrane transport
A	1990031	cellular_component	pinceau fiber
A	2000008	biological_process	regulation of protein localization to cell surface
B	0005216	molecular_function	monoatomic ion channel activity
B	0005249	molecular_function	voltage-gated potassium channel activity
B	0006811	biological_process	monoatomic ion transport
B	0006813	biological_process	potassium ion transport
B	0008076	cellular_component	voltage-gated potassium channel complex
B	0016020	cellular_component	membrane
B	0051260	biological_process	protein homooligomerization
B	0055085	biological_process	transmembrane transport
C	0002244	biological_process	hematopoietic progenitor cell differentiation
C	0004033	molecular_function	aldo-keto reductase (NADPH) activity
C	0005249	molecular_function	voltage-gated potassium channel activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005886	cellular_component	plasma membrane
C	0006813	biological_process	potassium ion transport
C	0008076	cellular_component	voltage-gated potassium channel complex
C	0009898	cellular_component	cytoplasmic side of plasma membrane
C	0014069	cellular_component	postsynaptic density
C	0015459	molecular_function	potassium channel regulator activity
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0030424	cellular_component	axon
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0034702	cellular_component	monoatomic ion channel complex
C	0034705	cellular_component	potassium channel complex
C	0043005	cellular_component	neuron projection
C	0043194	cellular_component	axon initial segment
C	0043679	cellular_component	axon terminus
C	0044224	cellular_component	juxtaparanode region of axon
C	0044325	molecular_function	transmembrane transporter binding
C	0044877	molecular_function	protein-containing complex binding
C	0045202	cellular_component	synapse
C	0045445	biological_process	myoblast differentiation
C	0050905	biological_process	neuromuscular process
C	0055085	biological_process	transmembrane transport
C	0055114	biological_process	obsolete oxidation-reduction process
C	0070995	biological_process	NADPH oxidation
C	0071805	biological_process	potassium ion transmembrane transport
C	0098839	cellular_component	postsynaptic density membrane
C	0098900	biological_process	regulation of action potential
C	0098978	cellular_component	glutamatergic synapse
C	1901379	biological_process	regulation of potassium ion transmembrane transport
C	1990031	cellular_component	pinceau fiber
C	2000008	biological_process	regulation of protein localization to cell surface
D	0005216	molecular_function	monoatomic ion channel activity
D	0005249	molecular_function	voltage-gated potassium channel activity
D	0006811	biological_process	monoatomic ion transport
D	0006813	biological_process	potassium ion transport
D	0008076	cellular_component	voltage-gated potassium channel complex
D	0016020	cellular_component	membrane
D	0051260	biological_process	protein homooligomerization
D	0055085	biological_process	transmembrane transport
E	0002244	biological_process	hematopoietic progenitor cell differentiation
E	0004033	molecular_function	aldo-keto reductase (NADPH) activity
E	0005249	molecular_function	voltage-gated potassium channel activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0008076	cellular_component	voltage-gated potassium channel complex
E	0009898	cellular_component	cytoplasmic side of plasma membrane
E	0014069	cellular_component	postsynaptic density
E	0015459	molecular_function	potassium channel regulator activity
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0030424	cellular_component	axon
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0034702	cellular_component	monoatomic ion channel complex
E	0034705	cellular_component	potassium channel complex
E	0043005	cellular_component	neuron projection
E	0043194	cellular_component	axon initial segment
E	0043679	cellular_component	axon terminus
E	0044224	cellular_component	juxtaparanode region of axon
E	0044325	molecular_function	transmembrane transporter binding
E	0044877	molecular_function	protein-containing complex binding
E	0045202	cellular_component	synapse
E	0045445	biological_process	myoblast differentiation
E	0050905	biological_process	neuromuscular process
E	0055085	biological_process	transmembrane transport
E	0055114	biological_process	obsolete oxidation-reduction process
E	0070995	biological_process	NADPH oxidation
E	0071805	biological_process	potassium ion transmembrane transport
E	0098839	cellular_component	postsynaptic density membrane
E	0098900	biological_process	regulation of action potential
E	0098978	cellular_component	glutamatergic synapse
E	1901379	biological_process	regulation of potassium ion transmembrane transport
E	1990031	cellular_component	pinceau fiber
E	2000008	biological_process	regulation of protein localization to cell surface
F	0005216	molecular_function	monoatomic ion channel activity
F	0005249	molecular_function	voltage-gated potassium channel activity
F	0006811	biological_process	monoatomic ion transport
F	0006813	biological_process	potassium ion transport
F	0008076	cellular_component	voltage-gated potassium channel complex
F	0016020	cellular_component	membrane
F	0051260	biological_process	protein homooligomerization
F	0055085	biological_process	transmembrane transport
G	0002244	biological_process	hematopoietic progenitor cell differentiation
G	0004033	molecular_function	aldo-keto reductase (NADPH) activity
G	0005249	molecular_function	voltage-gated potassium channel activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0005886	cellular_component	plasma membrane
G	0006813	biological_process	potassium ion transport
G	0008076	cellular_component	voltage-gated potassium channel complex
G	0009898	cellular_component	cytoplasmic side of plasma membrane
G	0014069	cellular_component	postsynaptic density
G	0015459	molecular_function	potassium channel regulator activity
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0030424	cellular_component	axon
G	0034220	biological_process	monoatomic ion transmembrane transport
G	0034702	cellular_component	monoatomic ion channel complex
G	0034705	cellular_component	potassium channel complex
G	0043005	cellular_component	neuron projection
G	0043194	cellular_component	axon initial segment
G	0043679	cellular_component	axon terminus
G	0044224	cellular_component	juxtaparanode region of axon
G	0044325	molecular_function	transmembrane transporter binding
G	0044877	molecular_function	protein-containing complex binding
G	0045202	cellular_component	synapse
G	0045445	biological_process	myoblast differentiation
G	0050905	biological_process	neuromuscular process
G	0055085	biological_process	transmembrane transport
G	0055114	biological_process	obsolete oxidation-reduction process
G	0070995	biological_process	NADPH oxidation
G	0071805	biological_process	potassium ion transmembrane transport
G	0098839	cellular_component	postsynaptic density membrane
G	0098900	biological_process	regulation of action potential
G	0098978	cellular_component	glutamatergic synapse
G	1901379	biological_process	regulation of potassium ion transmembrane transport
G	1990031	cellular_component	pinceau fiber
G	2000008	biological_process	regulation of protein localization to cell surface
H	0005216	molecular_function	monoatomic ion channel activity
H	0005249	molecular_function	voltage-gated potassium channel activity
H	0006811	biological_process	monoatomic ion transport
H	0006813	biological_process	potassium ion transport
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0016020	cellular_component	membrane
H	0051260	biological_process	protein homooligomerization
H	0055085	biological_process	transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	28
Details	binding site for residue NAP A 1001

Chain	Residue
A	GLY55
A	GLN214
A	TRP243
A	SER244
A	PRO245
A	LEU246
A	ALA247
A	CYS248
A	GLY249
A	LYS254
A	SER263
A	THR56
A	ARG264
A	PRO304
A	LEU321
A	LEU322
A	GLY323
A	SER325
A	GLN329
A	GLU332
A	ASN333
A	TRP57
A	ASP85
A	TYR90
A	LYS118
A	ASN158
A	SER188
A	ARG189

---

site_id	AC2
Number of Residues	29
Details	binding site for residue NAP C 1001

Chain	Residue
C	GLY55
C	THR56
C	TRP57
C	THR59
C	GLN63
C	TYR90
C	LYS118
C	ASN158
C	SER188
C	ARG189
C	GLN214
C	TRP243
C	SER244
C	PRO245
C	LEU246
C	ALA247
C	CYS248
C	GLY249
C	LYS254
C	TYR262
C	SER263
C	ARG264
C	PRO304
C	LEU322
C	GLY323
C	SER325
C	GLN329
C	GLU332
C	ASN333

---

site_id	AC3
Number of Residues	29
Details	binding site for residue NAP E 1001

Chain	Residue
E	GLY55
E	THR56
E	TRP57
E	THR59
E	GLN63
E	TYR90
E	LYS118
E	ASN158
E	SER188
E	ARG189
E	GLN214
E	TRP243
E	SER244
E	PRO245
E	LEU246
E	ALA247
E	CYS248
E	GLY249
E	LYS254
E	TYR262
E	SER263
E	ARG264
E	PRO304
E	LEU322
E	GLY323
E	SER325
E	GLN329
E	GLU332
E	ASN333

---

site_id	AC4
Number of Residues	29
Details	binding site for residue NAP G 1001

Chain	Residue
G	PRO245
G	LEU246
G	ALA247
G	CYS248
G	GLY249
G	LYS254
G	TYR262
G	SER263
G	ARG264
G	PRO304
G	LEU322
G	GLY323
G	SER325
G	GLN329
G	GLU332
G	ASN333
G	GLY55
G	THR56
G	TRP57
G	THR59
G	GLN63
G	TYR90
G	LYS118
G	ASN158
G	SER188
G	ARG189
G	GLN214
G	TRP243
G	SER244

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	732
Details	TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	MET1-GLY160
H	MET1-GLY160
H	CYS244-ILE254
H	SER307-MET321
B	CYS244-ILE254
B	SER307-MET321
D	MET1-GLY160
D	CYS244-ILE254
D	SER307-MET321
F	MET1-GLY160
F	CYS244-ILE254
F	SER307-MET321

---

site_id	SWS_FT_FI2
Number of Residues	84
Details	TRANSMEM: Helical; Name=Segment S1 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	PRO161-LEU182
A	LYS254
A	ARG264
A	SER325
A	GLN329
A	ASN333
C	THR56
C	TRP57
C	GLN63
C	ASP85
C	SER188
D	PRO161-LEU182
C	GLN214
C	TRP243
C	SER244
C	LEU246
C	LYS254
C	ARG264
C	SER325
C	GLN329
C	ASN333
E	THR56
F	PRO161-LEU182
E	TRP57
E	GLN63
E	ASP85
E	SER188
E	GLN214
E	TRP243
E	SER244
E	LEU246
E	LYS254
E	ARG264
H	PRO161-LEU182
E	SER325
E	GLN329
E	ASN333
G	THR56
G	TRP57
G	GLN63
G	ASP85
G	SER188
G	GLN214
G	TRP243
A	SER188
G	SER244
G	LEU246
G	LYS254
G	ARG264
G	SER325
G	GLN329
G	ASN333
A	GLN214
A	TRP243
A	SER244
A	LEU246

---

site_id	SWS_FT_FI3
Number of Residues	152
Details	TOPO_DOM: Extracellular => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430, ECO:0000305|PubMed:12151401

Chain	Residue	Details
B	GLU183-PRO221
D	GLU183-PRO221
F	GLU183-PRO221
H	GLU183-PRO221

---

site_id	SWS_FT_FI4
Number of Residues	84
Details	TRANSMEM: Helical; Name=Segment S2 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	PHE222-ALA243
D	PHE222-ALA243
F	PHE222-ALA243
H	PHE222-ALA243

---

site_id	SWS_FT_FI5
Number of Residues	84
Details	TRANSMEM: Helical; Name=Segment S5 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	ARG322-TYR343
D	ARG322-TYR343
F	ARG322-TYR343
H	ARG322-TYR343

---

site_id	SWS_FT_FI6
Number of Residues	76
Details	TOPO_DOM: Extracellular => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	PHE344-ILE357
B	PRO378-LYS384
D	PHE344-ILE357
D	PRO378-LYS384
F	PHE344-ILE357
F	PRO378-LYS384
H	PHE344-ILE357
H	PRO378-LYS384

---

site_id	SWS_FT_FI7
Number of Residues	44
Details	INTRAMEM: Helical; Name=Pore helix => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	PRO358-THR369
D	PRO358-THR369
F	PRO358-THR369
H	PRO358-THR369

---

site_id	SWS_FT_FI8
Number of Residues	28
Details	INTRAMEM: INTRAMEM => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	THR370-VAL377
D	THR370-VAL377
F	THR370-VAL377
H	THR370-VAL377

---

site_id	SWS_FT_FI9
Number of Residues	112
Details	TRANSMEM: Helical; Name=Segment S6 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430

Chain	Residue	Details
B	ILE385-TYR413
D	ILE385-TYR413
F	ILE385-TYR413
H	ILE385-TYR413

---

site_id	SWS_FT_FI10
Number of Residues	324
Details	TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:12151401, ECO:0000305

Chain	Residue	Details
B	HIS414-VAL495
D	HIS414-VAL495
F	HIS414-VAL495
H	HIS414-VAL495

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	SITE: Important for normal, slow channel gating => ECO:0000269|PubMed:17766348

Chain	Residue	Details
B	THR252
D	THR252
F	THR252
H	THR252

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	SITE: Important for binding with the scorpion mesomartoxin; when the scorpion mesomartoxin-rKv1.2/KCNA2 interaction is modeled, this residue is close to the 'Y-57' residue of the toxin => ECO:0000305|PubMed:25514171

Chain	Residue	Details
B	VAL377
D	VAL377
F	VAL377
H	VAL377

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P63141

Chain	Residue	Details
B	TYR425
D	TYR425
F	TYR425
H	TYR425

---

site_id	SWS_FT_FI14
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P63141

Chain	Residue	Details
B	SER430
D	SER430
F	SER430
H	SER430

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0007744|PubMed:22673903

Chain	Residue	Details
B	SER436
D	SER436
F	SER436
H	SER436

---

site_id	SWS_FT_FI16
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:22673903

Chain	Residue	Details
B	SER437
B	SER464
D	SER437
D	SER464
F	SER437
F	SER464
H	SER437
H	SER464

---

site_id	SWS_FT_FI17
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0000269|PubMed:21602278

Chain	Residue	Details
B	SER445
D	SER445
F	SER445
H	SER445

---

site_id	SWS_FT_FI18
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000305|PubMed:21602278

Chain	Residue	Details
B	TYR454
D	TYR454
F	TYR454
H	TYR454

---

site_id	SWS_FT_FI19
Number of Residues	4
Details	LIPID: S-palmitoyl cysteine => ECO:0000255

Chain	Residue	Details
B	CYS244
D	CYS244
F	CYS244
H	CYS244

---

site_id	SWS_FT_FI20
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:16770729

Chain	Residue	Details
B	GLN207
D	GLN207
F	GLN207
H	GLN207

---

site_id	SWS_FT_FI21
Number of Residues	44
Details	TOPO_DOM: Extracellular => ECO:0000250|UniProtKB:P63142

Chain	Residue	Details
B	GLU274-VAL285
D	GLU274-VAL285
F	GLU274-VAL285
H	GLU274-VAL285

---

site_id	SWS_FT_FI22
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
B	ASN276
D	ASN276
F	ASN276
H	ASN276

---