6EA9

Structure of VACV Poxin in post-reactive state with Gp[2'-5']Ap[3']

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004518	molecular_function	nuclease activity
A	0030430	cellular_component	host cell cytoplasm
A	0052170	biological_process	symbiont-mediated suppression of host innate immune response
A	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
B	0004518	molecular_function	nuclease activity
B	0030430	cellular_component	host cell cytoplasm
B	0052170	biological_process	symbiont-mediated suppression of host innate immune response
B	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
C	0004518	molecular_function	nuclease activity
C	0030430	cellular_component	host cell cytoplasm
C	0052170	biological_process	symbiont-mediated suppression of host innate immune response
C	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
D	0004518	molecular_function	nuclease activity
D	0030430	cellular_component	host cell cytoplasm
D	0052170	biological_process	symbiont-mediated suppression of host innate immune response
D	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
E	0004518	molecular_function	nuclease activity
E	0030430	cellular_component	host cell cytoplasm
E	0052170	biological_process	symbiont-mediated suppression of host innate immune response
E	0061507	molecular_function	2',3'-cyclic GMP-AMP binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue 9BG A 301

Chain	Residue
A	ALA129
A	LYS186
A	HOH419
A	HOH422
B	GLU14
B	LEU16
B	HIS17
B	ALA18
B	ALA27
B	ARG60
B	ILE105
A	VAL130
A	TYR138
A	LYS142
A	ALA145
A	ASN149
A	GLN169
A	ARG182
A	ARG184

---

site_id	AC2
Number of Residues	22
Details	binding site for residue 9BG B 301

Chain	Residue
A	GLU14
A	LEU16
A	HIS17
A	ALA18
A	PHE19
A	ALA27
A	ARG60
A	ILE105
B	ALA129
B	VAL130
B	TYR138
B	LYS142
B	ALA145
B	ASN149
B	GLN169
B	ARG182
B	ARG184
B	LYS186
B	HOH417
B	HOH424
B	HOH432
B	HOH434

---

site_id	AC3
Number of Residues	17
Details	binding site for residue 9BG C 301

Chain	Residue
C	ALA129
C	VAL130
C	TYR138
C	LYS142
C	ALA145
C	ASN149
C	GLN169
C	ARG182
C	ARG184
C	LYS186
D	GLU14
D	LEU16
D	HIS17
D	ALA18
D	ALA27
D	ARG60
D	ILE105

---

site_id	AC4
Number of Residues	18
Details	binding site for residue 9BG D 301

Chain	Residue
C	GLU14
C	LEU16
C	HIS17
C	ALA18
C	ALA27
C	ARG60
C	ILE105
D	ALA129
D	VAL130
D	TYR138
D	LYS142
D	ASN149
D	GLN169
D	ARG182
D	ARG184
D	LYS186
D	HOH402
D	HOH407

---

site_id	AC5
Number of Residues	17
Details	binding site for residue 9BG E 301

Chain	Residue
E	GLU14
E	LEU16
E	HIS17
E	ALA18
E	PHE19
E	ALA27
E	ARG60
E	ALA129
E	VAL130
E	TYR138
E	LYS142
E	ALA145
E	ASN149
E	GLN169
E	ARG182
E	ARG184
E	LYS186

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_04143, ECO:0000269|PubMed:30728498

Chain	Residue	Details
A	HIS17
B	HIS17
C	HIS17
D	HIS17
E	HIS17

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	ACT_SITE: Shared with catalytic histidine of dimeric partner => ECO:0000255|HAMAP-Rule:MF_04143, ECO:0000269|PubMed:30728498

Chain	Residue	Details
A	TYR138
B	TYR138
C	TYR138
D	TYR138
E	TYR138

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	ACT_SITE: Proton acceptor; shared with catalytic histidine of dimeric partner => ECO:0000255|HAMAP-Rule:MF_04143, ECO:0000269|PubMed:30728498

Chain	Residue	Details
A	LYS142
B	LYS142
C	LYS142
D	LYS142
E	LYS142

---

site_id	SWS_FT_FI4
Number of Residues	35
Details	SITE: Substrate binding => ECO:0000255|HAMAP-Rule:MF_04143, ECO:0000269|PubMed:30728498

Chain	Residue	Details
A	ARG60
B	ASN149
B	GLN169
B	ARG182
B	ARG184
B	LYS186
C	ARG60
C	ILE105
C	ASN149
C	GLN169
C	ARG182
A	ILE105
C	ARG184
C	LYS186
D	ARG60
D	ILE105
D	ASN149
D	GLN169
D	ARG182
D	ARG184
D	LYS186
E	ARG60
A	ASN149
E	ILE105
E	ASN149
E	GLN169
E	ARG182
E	ARG184
E	LYS186
A	GLN169
A	ARG182
A	ARG184
A	LYS186
B	ARG60
B	ILE105

---