Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6DEC

Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003779	molecular_function	actin binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005885	cellular_component	Arp2/3 protein complex
A	0010592	biological_process	positive regulation of lamellipodium assembly
A	0030030	biological_process	cell projection organization
A	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
A	0035861	cellular_component	site of double-strand break
A	0042995	cellular_component	cell projection
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0051015	molecular_function	actin filament binding
A	0060271	biological_process	cilium assembly
B	0003779	molecular_function	actin binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005885	cellular_component	Arp2/3 protein complex
B	0005938	cellular_component	cell cortex
B	0010592	biological_process	positive regulation of lamellipodium assembly
B	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
B	0035861	cellular_component	site of double-strand break
B	0042995	cellular_component	cell projection
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0051015	molecular_function	actin filament binding
B	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
B	2001032	biological_process	regulation of double-strand break repair via nonhomologous end joining
C	0003779	molecular_function	actin binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005885	cellular_component	Arp2/3 protein complex
C	0015629	cellular_component	actin cytoskeleton
C	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
C	0051015	molecular_function	actin filament binding
D	0003779	molecular_function	actin binding
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005885	cellular_component	Arp2/3 protein complex
D	0015629	cellular_component	actin cytoskeleton
D	0030041	biological_process	actin filament polymerization
D	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
D	0035861	cellular_component	site of double-strand break
D	0042995	cellular_component	cell projection
D	0043005	cellular_component	neuron projection
D	0045202	cellular_component	synapse
D	0051015	molecular_function	actin filament binding
E	0003779	molecular_function	actin binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005885	cellular_component	Arp2/3 protein complex
E	0030833	biological_process	regulation of actin filament polymerization
E	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
E	0035861	cellular_component	site of double-strand break
E	0042995	cellular_component	cell projection
E	0051015	molecular_function	actin filament binding
F	0003779	molecular_function	actin binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005885	cellular_component	Arp2/3 protein complex
F	0015629	cellular_component	actin cytoskeleton
F	0030041	biological_process	actin filament polymerization
F	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
F	0035861	cellular_component	site of double-strand break
F	0042995	cellular_component	cell projection
F	0051015	molecular_function	actin filament binding
G	0003779	molecular_function	actin binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005885	cellular_component	Arp2/3 protein complex
G	0015629	cellular_component	actin cytoskeleton
G	0016477	biological_process	cell migration
G	0030833	biological_process	regulation of actin filament polymerization
G	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
G	0035861	cellular_component	site of double-strand break
G	0042995	cellular_component	cell projection
G	0051015	molecular_function	actin filament binding
H	0003779	molecular_function	actin binding
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005885	cellular_component	Arp2/3 protein complex
H	0010592	biological_process	positive regulation of lamellipodium assembly
H	0030030	biological_process	cell projection organization
H	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
H	0035861	cellular_component	site of double-strand break
H	0042995	cellular_component	cell projection
H	0045944	biological_process	positive regulation of transcription by RNA polymerase II
H	0051015	molecular_function	actin filament binding
H	0060271	biological_process	cilium assembly
I	0003779	molecular_function	actin binding
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005856	cellular_component	cytoskeleton
I	0005885	cellular_component	Arp2/3 protein complex
I	0005938	cellular_component	cell cortex
I	0010592	biological_process	positive regulation of lamellipodium assembly
I	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
I	0035861	cellular_component	site of double-strand break
I	0042995	cellular_component	cell projection
I	0045944	biological_process	positive regulation of transcription by RNA polymerase II
I	0051015	molecular_function	actin filament binding
I	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
I	2001032	biological_process	regulation of double-strand break repair via nonhomologous end joining
J	0003779	molecular_function	actin binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005856	cellular_component	cytoskeleton
J	0005885	cellular_component	Arp2/3 protein complex
J	0015629	cellular_component	actin cytoskeleton
J	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
J	0051015	molecular_function	actin filament binding
K	0003779	molecular_function	actin binding
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005856	cellular_component	cytoskeleton
K	0005885	cellular_component	Arp2/3 protein complex
K	0015629	cellular_component	actin cytoskeleton
K	0030041	biological_process	actin filament polymerization
K	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
K	0035861	cellular_component	site of double-strand break
K	0042995	cellular_component	cell projection
K	0043005	cellular_component	neuron projection
K	0045202	cellular_component	synapse
K	0051015	molecular_function	actin filament binding
L	0003779	molecular_function	actin binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005856	cellular_component	cytoskeleton
L	0005885	cellular_component	Arp2/3 protein complex
L	0030833	biological_process	regulation of actin filament polymerization
L	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
L	0035861	cellular_component	site of double-strand break
L	0042995	cellular_component	cell projection
L	0051015	molecular_function	actin filament binding
N	0003779	molecular_function	actin binding
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005856	cellular_component	cytoskeleton
N	0005885	cellular_component	Arp2/3 protein complex
N	0015629	cellular_component	actin cytoskeleton
N	0030041	biological_process	actin filament polymerization
N	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
N	0035861	cellular_component	site of double-strand break
N	0042995	cellular_component	cell projection
N	0051015	molecular_function	actin filament binding
O	0003779	molecular_function	actin binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0005885	cellular_component	Arp2/3 protein complex
O	0015629	cellular_component	actin cytoskeleton
O	0016477	biological_process	cell migration
O	0030833	biological_process	regulation of actin filament polymerization
O	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
O	0035861	cellular_component	site of double-strand break
O	0042995	cellular_component	cell projection
O	0051015	molecular_function	actin filament binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	binding site for residue CA A 501

Chain	Residue
A	ATP502

site_id	AC2
Number of Residues	13
Details	binding site for residue ATP A 502

Chain	Residue
A	ARG198
A	GLY323
A	GLY324
A	SER325
A	CA501
A	GLY13
A	THR14
A	GLY15
A	TYR16
A	SER170
A	GLY171
A	ASP172
A	GLY197

site_id	AC3
Number of Residues	1
Details	binding site for residue CA B 501

Chain	Residue
B	ATP502

site_id	AC4
Number of Residues	12
Details	binding site for residue ATP B 502

Chain	Residue
B	GLY14
B	THR15
B	GLY16
B	PHE17
B	GLY160
B	ASP161
B	GLY186
B	GLY305
B	GLY306
B	MET309
B	TYR310
B	CA501

site_id	AC5
Number of Residues	2
Details	binding site for residue CA H 501

Chain	Residue
H	GLY13
H	ATP502

site_id	AC6
Number of Residues	7
Details	binding site for residue ATP H 502

Chain	Residue
H	GLY13
H	THR14
H	GLY15
H	TYR16
H	LYS228
H	SER325
H	CA501

site_id	AC7
Number of Residues	1
Details	binding site for residue CA I 501

Chain	Residue
I	ATP502

site_id	AC8
Number of Residues	8
Details	binding site for residue ATP I 502

Chain	Residue
I	GLY160
I	ASP161
I	GLY162
I	GLY186
I	GLU218
I	GLY306
I	MET309
I	CA501

Functional Information from PROSITE/UniProt

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEPpLNTpeNR

Chain	Residue	Details
A	LEU111-ARG123
B	LEU108-ARG120

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q9ESJ4

Chain	Residue	Details
M	SER294
P	SER294
K	LYS275
K	LYS295
I	ARG214
I	GLY305

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:O15145

Chain	Residue	Details
E	LYS56
E	LYS61
L	LYS56
L	LYS61
H	LYS240
H	LYS244
H	LYS251
H	LYS254

site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250\|UniProtKB:O15145

Chain	Residue	Details
E	LYS14
B	LYS322
L	LYS14
I	LYS322

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)