6DEC
Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791829 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 179.840, 197.381, 202.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.939 - 4.600 |
R-factor | 0.2767 |
Rwork | 0.275 |
R-free | 0.31410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jd2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.478 |
Data reduction software | HKL-3000 (2.3.6) |
Data scaling software | HKL-3000 (2.3.6) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.680 |
High resolution limit [Å] | 4.600 | 4.600 |
Rpim | 0.073 | 0.671 |
Number of reflections | 39413 | 1921 |
<I/σ(I)> | 11.8 | 1.5 |
Completeness [%] | 93.9 | 93.2 |
Redundancy | 5.4 | 4.7 |
CC(1/2) | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT |