6CU6

Crystal structure of GMPPNP-bound G12R mutant of human KRAS4b

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
B	0007165	biological_process	signal transduction
B	0016020	cellular_component	membrane
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MG A 401

Chain	Residue
A	SER17
A	THR35
A	GNP402
A	HOH519
A	HOH541

---

site_id	AC2
Number of Residues	26
Details	binding site for residue GNP A 402

Chain	Residue
A	LYS16
A	SER17
A	ALA18
A	PHE28
A	VAL29
A	ASP30
A	GLU31
A	TYR32
A	THR35
A	GLY60
A	ASN116
A	LYS117
A	ASP119
A	LEU120
A	SER145
A	ALA146
A	MG401
A	HOH519
A	HOH527
A	HOH541
A	HOH553
A	HOH568
A	ARG12
A	GLY13
A	VAL14
A	GLY15

---

site_id	AC3
Number of Residues	5
Details	binding site for residue MG B 401

Chain	Residue
B	SER17
B	THR35
B	GNP403
B	HOH501
B	HOH528

---

site_id	AC4
Number of Residues	4
Details	binding site for residue GOL B 402

Chain	Residue
A	ARG149
B	ASP33
B	ASP38
B	TYR40

---

site_id	AC5
Number of Residues	26
Details	binding site for residue GNP B 403

Chain	Residue
B	ARG12
B	GLY13
B	VAL14
B	GLY15
B	LYS16
B	SER17
B	ALA18
B	PHE28
B	VAL29
B	ASP30
B	GLU31
B	TYR32
B	THR35
B	ALA59
B	ASN116
B	LYS117
B	ASP119
B	LEU120
B	SER145
B	ALA146
B	LYS147
B	MG401
B	HOH501
B	HOH522
B	HOH528
B	HOH533

---

site_id	AC6
Number of Residues	4
Details	binding site for residue MG C 401

Chain	Residue
C	SER17
C	ASP57
C	GNP402
C	HOH530

---

site_id	AC7
Number of Residues	19
Details	binding site for residue GNP C 402

Chain	Residue
C	ARG12
C	GLY13
C	VAL14
C	GLY15
C	LYS16
C	SER17
C	ALA18
C	PHE28
C	VAL29
C	ASN116
C	LYS117
C	ASP119
C	LEU120
C	SER145
C	ALA146
C	LYS147
C	MG401
C	HOH530
C	HOH545

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Motif: {"description":"Effector region"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	47
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22431598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22566140","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34380736","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"N-acetylmethionine; in GTPase KRas; alternate","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Modified residue: {"description":"N-acetylthreonine; in GTPase KRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"22711838","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---