6BUU

Crystal structure of AKT1 (aa 144-480) with a bisubstrate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue SO4 A 501

Chain	Residue
A	ASN351
A	GLN352

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site_id	AC2
Number of Residues	2
Details	binding site for residue MN F 501

Chain	Residue
A	ASP292
F	ZXW7

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site_id	AC3
Number of Residues	3
Details	binding site for residue MN G 501

Chain	Residue
B	ASN279
B	ASP292
G	ZXW7

---

site_id	AC4
Number of Residues	21
Details	binding site for Ligand residues ZXW F 7 through PHE F 8 bound to THR F 6

Chain	Residue
A	VAL164
A	LYS179
A	GLU228
A	ALA230
A	GLU234
A	ASP274
A	LYS276
A	MET281
A	ASP292
A	PHE309
A	CYS310
A	GLY311
A	THR312
A	PHE438
F	ARG4
F	THR6
F	ALA9
F	MN501
F	HOH601
A	GLY159
A	THR160

---

site_id	AC5
Number of Residues	23
Details	binding site for Ligand residues ZXW G 7 through PHE G 8 bound to THR G 6

Chain	Residue
B	GLY159
B	THR160
B	VAL164
B	ALA177
B	LYS179
B	GLU228
B	ALA230
B	GLU234
B	ASP274
B	LYS276
B	MET281
B	ASP292
B	LEU295
B	PHE309
B	CYS310
B	GLY311
B	THR312
B	PHE438
G	THR6
G	ALA9
G	MN501
G	HOH601
G	HOH603

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	34
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGKGTFGKVIlVkekatgryyamkilkkevIVAK

Chain	Residue	Details
A	LEU156-LYS189

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvYrDLKleNLML

Chain	Residue	Details
A	VAL270-LEU282

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PKB/AKT1, RPS6KA3 and SGK3 => ECO:0000269|PubMed:12054501, ECO:0000269|PubMed:16484495, ECO:0000269|PubMed:20937854, ECO:0000269|PubMed:24391509, ECO:0000269|PubMed:25169422, ECO:0000269|PubMed:35606353, ECO:0000269|PubMed:8250835

Chain	Residue	Details
F	ZXW7
G	ZXW7

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
A	LEU156
A	LYS179
B	LEU156
B	LYS179

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Cleavage; by caspase-3 => ECO:0000250|UniProtKB:P31750

Chain	Residue	Details
A	ASP462
B	ASP462

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphotyrosine; by TNK2 => ECO:0000269|PubMed:20333297

Chain	Residue	Details
A	TYR176
B	TYR176

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by IKKE, PDPK1 and TBK1 => ECO:0000269|PubMed:15718470, ECO:0000269|PubMed:18456494, ECO:0000269|PubMed:20333297, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:21464307, ECO:0000269|PubMed:8978681, ECO:0000269|PubMed:9512493

Chain	Residue	Details
A	TPO308
B	TPO308

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	THR448
A	THR450
B	THR448
B	THR450

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine; by IKKE, MTOR and TBK1; alternate => ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15047712, ECO:0000269|PubMed:15718470, ECO:0000269|PubMed:16266983, ECO:0000269|PubMed:17013611, ECO:0000269|PubMed:20333297, ECO:0000269|PubMed:20978158, ECO:0000269|PubMed:21464307, ECO:0000269|PubMed:23799035, ECO:0000269|PubMed:8978681, ECO:0000269|PubMed:9736715

Chain	Residue	Details
A	SEP473
B	SEP473

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:12149249

Chain	Residue	Details
A	TYR474
B	TYR474

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CARBOHYD: O-linked (GlcNAc) threonine => ECO:0000269|PubMed:22629392

Chain	Residue	Details
A	THR305
A	THR312
B	THR305
B	THR312

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine; alternate => ECO:0000250|UniProtKB:P31750

Chain	Residue	Details
A	SEP473
B	SEP473

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:22410793

Chain	Residue	Details
A	LYS284
B	LYS284

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