6BAD
Lactate Dehydrogenase in complex with inhibitor (R)-3-((2-chlorophenyl)thio)-6-(3-((4-fluorophenyl)amino)phenyl)-4-hydroxy-6-(thiophen-3-yl)-5,6-dihydro-2H-pyran-2-one
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0006090 | biological_process | pyruvate metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0045296 | molecular_function | cadherin binding |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 1990204 | cellular_component | oxidoreductase complex |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0006090 | biological_process | pyruvate metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0045296 | molecular_function | cadherin binding |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 1990204 | cellular_component | oxidoreductase complex |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005829 | cellular_component | cytosol |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0006090 | biological_process | pyruvate metabolic process |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0045296 | molecular_function | cadherin binding |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 1990204 | cellular_component | oxidoreductase complex |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005829 | cellular_component | cytosol |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0006090 | biological_process | pyruvate metabolic process |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0045296 | molecular_function | cadherin binding |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 1990204 | cellular_component | oxidoreductase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 28 |
| Details | binding site for residue NAD A 801 |
| Chain | Residue |
| A | GLY28 |
| A | ALA97 |
| A | ARG98 |
| A | ILE115 |
| A | VAL135 |
| A | SER136 |
| A | ASN137 |
| A | HIS192 |
| A | THR247 |
| A | ILE251 |
| A | HOH907 |
| A | ALA29 |
| A | HOH917 |
| A | HOH926 |
| A | HOH927 |
| A | HOH945 |
| A | HOH971 |
| A | HOH981 |
| A | HOH1005 |
| A | HOH1011 |
| A | HOH1036 |
| A | VAL30 |
| A | ASP51 |
| A | VAL52 |
| A | ILE53 |
| A | THR94 |
| A | ALA95 |
| A | GLY96 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue EPE A 802 |
| Chain | Residue |
| A | TRP147 |
| A | GLY151 |
| A | LYS154 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | binding site for residue EPE A 803 |
| Chain | Residue |
| A | GLN99 |
| A | ARG105 |
| A | ASN137 |
| A | PRO138 |
| A | TYR238 |
| A | D0Y805 |
| A | HOH956 |
| A | HOH961 |
| A | HOH1039 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 A 804 |
| Chain | Residue |
| A | ARG170 |
| A | HIS185 |
| A | HOH919 |
| A | HOH995 |
| A | HOH1004 |
| C | LEU182 |
| C | HIS185 |
| site_id | AC5 |
| Number of Residues | 15 |
| Details | binding site for residue D0Y A 805 |
| Chain | Residue |
| A | ARG98 |
| A | GLN99 |
| A | GLN100 |
| A | ASN137 |
| A | LEU164 |
| A | ASP165 |
| A | ARG168 |
| A | HIS192 |
| A | GLY193 |
| A | ALA237 |
| A | TYR238 |
| A | ILE241 |
| A | THR247 |
| A | EPE803 |
| A | HOH1048 |
| site_id | AC6 |
| Number of Residues | 29 |
| Details | binding site for residue NAD B 801 |
| Chain | Residue |
| B | GLY28 |
| B | ALA29 |
| B | VAL30 |
| B | ASP51 |
| B | VAL52 |
| B | ILE53 |
| B | LYS56 |
| B | THR94 |
| B | ALA95 |
| B | GLY96 |
| B | ALA97 |
| B | ARG98 |
| B | ILE115 |
| B | ILE119 |
| B | VAL135 |
| B | SER136 |
| B | ASN137 |
| B | HIS192 |
| B | THR247 |
| B | ILE251 |
| B | HOH906 |
| B | HOH917 |
| B | HOH918 |
| B | HOH947 |
| B | HOH954 |
| B | HOH955 |
| B | HOH976 |
| B | HOH1002 |
| D | GLY102 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue EPE B 802 |
| Chain | Residue |
| A | LYS283 |
| B | TRP147 |
| B | GLY151 |
| B | PRO153 |
| B | LYS154 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | binding site for residue EPE B 803 |
| Chain | Residue |
| B | ASN137 |
| B | PRO138 |
| B | TYR238 |
| B | D0Y805 |
| B | HOH915 |
| B | HOH970 |
| B | GLN99 |
| B | ARG105 |
| B | LEU108 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 B 804 |
| Chain | Residue |
| B | ARG170 |
| B | HIS185 |
| B | HOH922 |
| B | HOH952 |
| B | HOH1000 |
| B | HOH1021 |
| D | HIS185 |
| site_id | AD1 |
| Number of Residues | 15 |
| Details | binding site for residue D0Y B 805 |
| Chain | Residue |
| B | GLN99 |
| B | GLN100 |
| B | ASN137 |
| B | LEU164 |
| B | ASP165 |
| B | ARG168 |
| B | HIS192 |
| B | GLY193 |
| B | ASP194 |
| B | ALA237 |
| B | TYR238 |
| B | ILE241 |
| B | THR247 |
| B | EPE803 |
| B | HOH970 |
| site_id | AD2 |
| Number of Residues | 28 |
| Details | binding site for residue NAD C 801 |
| Chain | Residue |
| C | GLY28 |
| C | ALA29 |
| C | VAL30 |
| C | ASP51 |
| C | VAL52 |
| C | ILE53 |
| C | LYS56 |
| C | THR94 |
| C | ALA95 |
| C | GLY96 |
| C | ALA97 |
| C | ARG98 |
| C | ILE115 |
| C | VAL135 |
| C | SER136 |
| C | ASN137 |
| C | HIS192 |
| C | THR247 |
| C | ILE251 |
| C | HOH905 |
| C | HOH910 |
| C | HOH916 |
| C | HOH938 |
| C | HOH939 |
| C | HOH944 |
| C | HOH960 |
| C | HOH989 |
| C | HOH1001 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue EPE C 802 |
| Chain | Residue |
| C | TRP147 |
| C | GLY151 |
| C | PRO153 |
| C | LYS154 |
| site_id | AD4 |
| Number of Residues | 9 |
| Details | binding site for residue EPE C 803 |
| Chain | Residue |
| C | GLN99 |
| C | ARG105 |
| C | ASN137 |
| C | PRO138 |
| C | TYR238 |
| C | D0Y807 |
| C | HOH975 |
| C | HOH979 |
| C | HOH1015 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 804 |
| Chain | Residue |
| C | HIS214 |
| C | PRO215 |
| C | ASP216 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 C 805 |
| Chain | Residue |
| A | HIS185 |
| C | ARG170 |
| C | HIS185 |
| C | HOH902 |
| C | HOH998 |
| C | HOH1017 |
| site_id | AD7 |
| Number of Residues | 3 |
| Details | binding site for residue EPE C 806 |
| Chain | Residue |
| C | ASP194 |
| C | GLU235 |
| C | TYR238 |
| site_id | AD8 |
| Number of Residues | 14 |
| Details | binding site for residue D0Y C 807 |
| Chain | Residue |
| C | ARG98 |
| C | GLN99 |
| C | GLN100 |
| C | ASN137 |
| C | ASP165 |
| C | ARG168 |
| C | HIS192 |
| C | GLY193 |
| C | ALA237 |
| C | TYR238 |
| C | ILE241 |
| C | THR247 |
| C | EPE803 |
| C | HOH979 |
| site_id | AD9 |
| Number of Residues | 29 |
| Details | binding site for residue NAD D 801 |
| Chain | Residue |
| D | GLY28 |
| D | ALA29 |
| D | VAL30 |
| D | ASP51 |
| D | VAL52 |
| D | ILE53 |
| D | LYS56 |
| D | THR94 |
| D | ALA95 |
| D | GLY96 |
| D | ALA97 |
| D | ARG98 |
| D | ILE115 |
| D | VAL135 |
| D | SER136 |
| D | ASN137 |
| D | HIS192 |
| D | THR247 |
| D | ILE251 |
| D | HOH906 |
| D | HOH918 |
| D | HOH926 |
| D | HOH931 |
| D | HOH935 |
| D | HOH945 |
| D | HOH946 |
| D | HOH968 |
| D | HOH983 |
| D | HOH999 |
| site_id | AE1 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 D 802 |
| Chain | Residue |
| B | HIS185 |
| B | HOH952 |
| D | ARG170 |
| D | HIS185 |
| D | HOH908 |
| D | HOH920 |
| D | HOH1015 |
| site_id | AE2 |
| Number of Residues | 13 |
| Details | binding site for residue D0Y D 803 |
| Chain | Residue |
| D | ARG98 |
| D | GLN99 |
| D | ARG105 |
| D | ASN137 |
| D | ASP165 |
| D | ARG168 |
| D | HIS192 |
| D | GLY193 |
| D | ALA237 |
| D | TYR238 |
| D | ILE241 |
| D | THR247 |
| D | HOH989 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. LGEHGDS |
| Chain | Residue | Details |
| A | LEU189-SER195 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | HIS192 | |
| B | HIS192 | |
| C | HIS192 | |
| D | HIS192 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11276087 |
| Chain | Residue | Details |
| A | GLY28 | |
| A | ARG98 | |
| B | GLY28 | |
| B | ARG98 | |
| C | GLY28 | |
| C | ARG98 | |
| D | GLY28 | |
| D | ARG98 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ARG105 | |
| C | ASN137 | |
| C | ARG168 | |
| C | THR247 | |
| D | ARG105 | |
| D | ASN137 | |
| D | ARG168 | |
| D | THR247 | |
| A | ASN137 | |
| A | ARG168 | |
| A | THR247 | |
| B | ARG105 | |
| B | ASN137 | |
| B | ARG168 | |
| B | THR247 | |
| C | ARG105 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|Ref.10, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895 |
| Chain | Residue | Details |
| A | ALA1 | |
| B | ALA1 | |
| C | ALA1 | |
| D | ALA1 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P06151 |
| Chain | Residue | Details |
| A | LYS4 | |
| D | LYS4 | |
| D | LYS117 | |
| D | LYS317 | |
| A | LYS117 | |
| A | LYS317 | |
| B | LYS4 | |
| B | LYS117 | |
| B | LYS317 | |
| C | LYS4 | |
| C | LYS117 | |
| C | LYS317 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| A | TYR9 | |
| B | TYR9 | |
| C | TYR9 | |
| D | TYR9 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS13 | |
| D | LYS13 | |
| D | LYS80 | |
| D | LYS125 | |
| A | LYS80 | |
| A | LYS125 | |
| B | LYS13 | |
| B | LYS80 | |
| B | LYS125 | |
| C | LYS13 | |
| C | LYS80 | |
| C | LYS125 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR17 | |
| B | THR17 | |
| C | THR17 | |
| D | THR17 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS56 | |
| B | LYS56 | |
| C | LYS56 | |
| D | LYS56 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P06151 |
| Chain | Residue | Details |
| A | LYS223 | |
| D | LYS223 | |
| D | LYS231 | |
| D | LYS242 | |
| A | LYS231 | |
| A | LYS242 | |
| B | LYS223 | |
| B | LYS231 | |
| B | LYS242 | |
| C | LYS223 | |
| C | LYS231 | |
| C | LYS242 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P06151 |
| Chain | Residue | Details |
| A | TYR238 | |
| B | TYR238 | |
| C | TYR238 | |
| D | TYR238 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P04642 |
| Chain | Residue | Details |
| A | THR308 | |
| A | THR321 | |
| B | THR308 | |
| B | THR321 | |
| C | THR308 | |
| C | THR321 | |
| D | THR308 | |
| D | THR321 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER309 | |
| B | SER309 | |
| C | SER309 | |
| D | SER309 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS56 | |
| B | LYS56 | |
| C | LYS56 | |
| D | LYS56 |
