6AJC
Crystal structure of Trypanosoma cruzi cytosolic isocitrate dehydrogenase in complex with NADP+, isocitrate and ca2+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005777 | cellular_component | peroxisome |
| A | 0005829 | cellular_component | cytosol |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0006102 | biological_process | isocitrate metabolic process |
| A | 0006739 | biological_process | NADP metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051287 | molecular_function | NAD binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005777 | cellular_component | peroxisome |
| B | 0005829 | cellular_component | cytosol |
| B | 0006099 | biological_process | tricarboxylic acid cycle |
| B | 0006102 | biological_process | isocitrate metabolic process |
| B | 0006739 | biological_process | NADP metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051287 | molecular_function | NAD binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005777 | cellular_component | peroxisome |
| C | 0005829 | cellular_component | cytosol |
| C | 0006099 | biological_process | tricarboxylic acid cycle |
| C | 0006102 | biological_process | isocitrate metabolic process |
| C | 0006739 | biological_process | NADP metabolic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051287 | molecular_function | NAD binding |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005777 | cellular_component | peroxisome |
| D | 0005829 | cellular_component | cytosol |
| D | 0006099 | biological_process | tricarboxylic acid cycle |
| D | 0006102 | biological_process | isocitrate metabolic process |
| D | 0006739 | biological_process | NADP metabolic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051287 | molecular_function | NAD binding |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| E | 0005739 | cellular_component | mitochondrion |
| E | 0005777 | cellular_component | peroxisome |
| E | 0005829 | cellular_component | cytosol |
| E | 0006099 | biological_process | tricarboxylic acid cycle |
| E | 0006102 | biological_process | isocitrate metabolic process |
| E | 0006739 | biological_process | NADP metabolic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0051287 | molecular_function | NAD binding |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0000287 | molecular_function | magnesium ion binding |
| F | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| F | 0005739 | cellular_component | mitochondrion |
| F | 0005777 | cellular_component | peroxisome |
| F | 0005829 | cellular_component | cytosol |
| F | 0006099 | biological_process | tricarboxylic acid cycle |
| F | 0006102 | biological_process | isocitrate metabolic process |
| F | 0006739 | biological_process | NADP metabolic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0051287 | molecular_function | NAD binding |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0000287 | molecular_function | magnesium ion binding |
| G | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| G | 0005739 | cellular_component | mitochondrion |
| G | 0005777 | cellular_component | peroxisome |
| G | 0005829 | cellular_component | cytosol |
| G | 0006099 | biological_process | tricarboxylic acid cycle |
| G | 0006102 | biological_process | isocitrate metabolic process |
| G | 0006739 | biological_process | NADP metabolic process |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0051287 | molecular_function | NAD binding |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0000287 | molecular_function | magnesium ion binding |
| H | 0004450 | molecular_function | isocitrate dehydrogenase (NADP+) activity |
| H | 0005739 | cellular_component | mitochondrion |
| H | 0005777 | cellular_component | peroxisome |
| H | 0005829 | cellular_component | cytosol |
| H | 0006099 | biological_process | tricarboxylic acid cycle |
| H | 0006102 | biological_process | isocitrate metabolic process |
| H | 0006739 | biological_process | NADP metabolic process |
| H | 0016491 | molecular_function | oxidoreductase activity |
| H | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| H | 0046872 | molecular_function | metal ion binding |
| H | 0051287 | molecular_function | NAD binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 22 |
| Details | binding site for residue NAP A 501 |
| Chain | Residue |
| A | LYS72 |
| A | HIS308 |
| A | GLY309 |
| A | THR310 |
| A | VAL311 |
| A | ARG313 |
| A | HIS314 |
| A | THR326 |
| A | ASN327 |
| A | ICT503 |
| B | THR213 |
| A | THR75 |
| B | LEU249 |
| B | ASP252 |
| B | LYS259 |
| A | ILE76 |
| A | THR77 |
| A | ARG82 |
| A | ASN96 |
| A | LEU287 |
| A | ALA306 |
| A | ALA307 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 502 |
| Chain | Residue |
| A | ARG109 |
| A | ASP274 |
| A | ASP278 |
| A | ICT503 |
| B | ASP251 |
| site_id | AC3 |
| Number of Residues | 13 |
| Details | binding site for residue ICT A 503 |
| Chain | Residue |
| A | THR77 |
| A | SER94 |
| A | ASN96 |
| A | ARG100 |
| A | ARG109 |
| A | ARG132 |
| A | TYR139 |
| A | ASP274 |
| A | ALA307 |
| A | NAP501 |
| A | CA502 |
| B | LYS211 |
| B | ASP251 |
| site_id | AC4 |
| Number of Residues | 24 |
| Details | binding site for residue NAP B 501 |
| Chain | Residue |
| A | LEU249 |
| A | ASP252 |
| A | LYS259 |
| A | HOH606 |
| B | LYS72 |
| B | ALA74 |
| B | THR75 |
| B | ILE76 |
| B | THR77 |
| B | ARG82 |
| B | ASN96 |
| B | LEU287 |
| B | ALA306 |
| B | ALA307 |
| B | HIS308 |
| B | GLY309 |
| B | THR310 |
| B | VAL311 |
| B | THR312 |
| B | ARG313 |
| B | HIS314 |
| B | ASN327 |
| B | ICT503 |
| B | HOH604 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue CA B 502 |
| Chain | Residue |
| A | ASP251 |
| B | ASP274 |
| B | ASP278 |
| B | ICT503 |
| B | HOH602 |
| site_id | AC6 |
| Number of Residues | 14 |
| Details | binding site for residue ICT B 503 |
| Chain | Residue |
| A | LYS211 |
| A | ILE214 |
| A | ASP251 |
| B | THR77 |
| B | SER94 |
| B | ASN96 |
| B | ARG100 |
| B | ARG109 |
| B | ARG132 |
| B | TYR139 |
| B | ASP274 |
| B | ALA307 |
| B | NAP501 |
| B | CA502 |
| site_id | AC7 |
| Number of Residues | 23 |
| Details | binding site for residue NAP C 501 |
| Chain | Residue |
| C | HIS314 |
| C | THR326 |
| C | ASN327 |
| C | ICT503 |
| D | LEU249 |
| D | ASP252 |
| D | LYS259 |
| C | LYS72 |
| C | ALA74 |
| C | THR75 |
| C | ILE76 |
| C | THR77 |
| C | ARG82 |
| C | ASN96 |
| C | LEU287 |
| C | ALA306 |
| C | ALA307 |
| C | HIS308 |
| C | GLY309 |
| C | THR310 |
| C | VAL311 |
| C | THR312 |
| C | ARG313 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue CA C 502 |
| Chain | Residue |
| C | ARG109 |
| C | ASP274 |
| C | ASP278 |
| C | ICT503 |
| D | ASP251 |
| site_id | AC9 |
| Number of Residues | 13 |
| Details | binding site for residue ICT C 503 |
| Chain | Residue |
| C | THR77 |
| C | SER94 |
| C | ASN96 |
| C | ARG100 |
| C | ARG109 |
| C | ARG132 |
| C | TYR139 |
| C | ASP274 |
| C | ALA307 |
| C | NAP501 |
| C | CA502 |
| D | LYS211 |
| D | ILE214 |
| site_id | AD1 |
| Number of Residues | 24 |
| Details | binding site for residue NAP D 501 |
| Chain | Residue |
| C | THR213 |
| C | LEU249 |
| C | ASP252 |
| C | LYS259 |
| D | LYS72 |
| D | ALA74 |
| D | THR75 |
| D | ILE76 |
| D | THR77 |
| D | ARG82 |
| D | ASN96 |
| D | LEU287 |
| D | ALA306 |
| D | ALA307 |
| D | HIS308 |
| D | GLY309 |
| D | THR310 |
| D | VAL311 |
| D | THR312 |
| D | ARG313 |
| D | HIS314 |
| D | THR326 |
| D | ASN327 |
| D | ICT503 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue CA D 502 |
| Chain | Residue |
| C | ASP251 |
| D | ASP274 |
| D | ASP278 |
| D | ICT503 |
| site_id | AD3 |
| Number of Residues | 12 |
| Details | binding site for residue ICT D 503 |
| Chain | Residue |
| C | LYS211 |
| C | ILE214 |
| D | THR77 |
| D | SER94 |
| D | ASN96 |
| D | ARG100 |
| D | ARG109 |
| D | ARG132 |
| D | TYR139 |
| D | ASP274 |
| D | NAP501 |
| D | CA502 |
| site_id | AD4 |
| Number of Residues | 25 |
| Details | binding site for residue NAP E 501 |
| Chain | Residue |
| E | LYS72 |
| E | ALA74 |
| E | THR75 |
| E | ILE76 |
| E | THR77 |
| E | ARG82 |
| E | ASN96 |
| E | LEU287 |
| E | ALA306 |
| E | ALA307 |
| E | HIS308 |
| E | GLY309 |
| E | THR310 |
| E | VAL311 |
| E | THR312 |
| E | ARG313 |
| E | HIS314 |
| E | ASN327 |
| E | ICT503 |
| E | HOH602 |
| E | HOH604 |
| F | THR213 |
| F | LEU249 |
| F | ASP252 |
| F | LYS259 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue CA E 502 |
| Chain | Residue |
| E | ARG109 |
| E | ASP274 |
| E | ASP278 |
| E | ICT503 |
| F | ASP251 |
| site_id | AD6 |
| Number of Residues | 13 |
| Details | binding site for residue ICT E 503 |
| Chain | Residue |
| E | THR77 |
| E | SER94 |
| E | ASN96 |
| E | ARG100 |
| E | ARG109 |
| E | ARG132 |
| E | TYR139 |
| E | ASP274 |
| E | ALA307 |
| E | NAP501 |
| E | CA502 |
| E | HOH604 |
| F | LYS211 |
| site_id | AD7 |
| Number of Residues | 20 |
| Details | binding site for residue NAP F 501 |
| Chain | Residue |
| E | LEU249 |
| E | ASP252 |
| E | LYS259 |
| F | ALA74 |
| F | THR75 |
| F | ILE76 |
| F | THR77 |
| F | ARG82 |
| F | ASN96 |
| F | ALA306 |
| F | ALA307 |
| F | HIS308 |
| F | GLY309 |
| F | THR310 |
| F | VAL311 |
| F | THR312 |
| F | ARG313 |
| F | HIS314 |
| F | ASN327 |
| F | ICT503 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue CA F 502 |
| Chain | Residue |
| E | ASP251 |
| F | ARG109 |
| F | ASP274 |
| F | ASP278 |
| F | ICT503 |
| site_id | AD9 |
| Number of Residues | 13 |
| Details | binding site for residue ICT F 503 |
| Chain | Residue |
| E | LYS211 |
| E | ASP251 |
| F | THR77 |
| F | SER94 |
| F | ASN96 |
| F | ARG100 |
| F | ARG109 |
| F | ARG132 |
| F | TYR139 |
| F | ASP274 |
| F | ALA307 |
| F | NAP501 |
| F | CA502 |
| site_id | AE1 |
| Number of Residues | 25 |
| Details | binding site for residue NAP G 501 |
| Chain | Residue |
| G | LYS72 |
| G | THR75 |
| G | ILE76 |
| G | THR77 |
| G | ARG82 |
| G | ASN96 |
| G | LEU287 |
| G | ALA306 |
| G | ALA307 |
| G | HIS308 |
| G | GLY309 |
| G | THR310 |
| G | VAL311 |
| G | THR312 |
| G | ARG313 |
| G | HIS314 |
| G | THR326 |
| G | ASN327 |
| G | ASP374 |
| G | ICT503 |
| G | HOH601 |
| G | HOH609 |
| H | LEU249 |
| H | ASP252 |
| H | LYS259 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue CA G 502 |
| Chain | Residue |
| G | ASP274 |
| G | ASP278 |
| G | ICT503 |
| G | HOH608 |
| H | ASP251 |
| site_id | AE3 |
| Number of Residues | 13 |
| Details | binding site for residue ICT G 503 |
| Chain | Residue |
| G | THR77 |
| G | SER94 |
| G | ASN96 |
| G | ARG100 |
| G | ARG109 |
| G | ARG132 |
| G | TYR139 |
| G | ASP274 |
| G | ALA307 |
| G | NAP501 |
| G | CA502 |
| H | LYS211 |
| H | ILE214 |
| site_id | AE4 |
| Number of Residues | 22 |
| Details | binding site for residue NAP H 501 |
| Chain | Residue |
| G | LEU249 |
| G | ASP252 |
| G | LYS259 |
| H | ALA74 |
| H | THR75 |
| H | ILE76 |
| H | THR77 |
| H | ARG82 |
| H | ASN96 |
| H | LEU287 |
| H | ALA306 |
| H | ALA307 |
| H | HIS308 |
| H | GLY309 |
| H | THR310 |
| H | VAL311 |
| H | THR312 |
| H | ARG313 |
| H | HIS314 |
| H | THR326 |
| H | ASN327 |
| H | ICT503 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue CA H 502 |
| Chain | Residue |
| G | ASP251 |
| H | ASP274 |
| H | ASP278 |
| H | ICT503 |
| site_id | AE6 |
| Number of Residues | 12 |
| Details | binding site for residue ICT H 503 |
| Chain | Residue |
| G | LYS211 |
| G | ILE214 |
| H | THR77 |
| H | SER94 |
| H | ASN96 |
| H | ARG100 |
| H | ARG109 |
| H | ARG132 |
| H | TYR139 |
| H | ASP274 |
| H | NAP501 |
| H | CA502 |
Functional Information from PROSITE/UniProt
| site_id | PS00470 |
| Number of Residues | 20 |
| Details | IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NYDGDVqSDsvAqgf.GSLGL |
| Chain | Residue | Details |
| A | ASN270-LEU289 |
