Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6AJA

Crystal structure of Trypanosoma brucei glycosomal isocitrate dehydrogenase in complex with NADPH, alpha-ketoglutarate and ca2+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0006099	biological_process	tricarboxylic acid cycle
A	0006102	biological_process	isocitrate metabolic process
A	0006739	biological_process	NADP+ metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0020015	cellular_component	glycosome
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0006099	biological_process	tricarboxylic acid cycle
B	0006102	biological_process	isocitrate metabolic process
B	0006739	biological_process	NADP+ metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0020015	cellular_component	glycosome
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0006099	biological_process	tricarboxylic acid cycle
C	0006102	biological_process	isocitrate metabolic process
C	0006739	biological_process	NADP+ metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0020015	cellular_component	glycosome
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0006099	biological_process	tricarboxylic acid cycle
D	0006102	biological_process	isocitrate metabolic process
D	0006739	biological_process	NADP+ metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0020015	cellular_component	glycosome
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue NAP A 501

Chain	Residue
A	LYS72
A	VAL311
A	THR312
A	ARG313
A	HIS314
A	ASN327
A	HOH620
A	THR75
A	ILE76
A	THR77
A	ARG82
A	ASN96
A	HIS308
A	GLY309
A	THR310

site_id	AC2
Number of Residues	3
Details	binding site for residue CA A 502

Chain	Residue
A	ASP274
A	ASP278
B	ASP251

site_id	AC3
Number of Residues	1
Details	binding site for residue GOL A 503

Chain	Residue
A	ASP360

site_id	AC4
Number of Residues	1
Details	binding site for residue GOL A 504

Chain	Residue
A	PRO118

site_id	AC5
Number of Residues	3
Details	binding site for residue CA A 505

Chain	Residue
A	ASP251
B	ASP274
B	ASP278

site_id	AC6
Number of Residues	13
Details	binding site for residue NAP B 501

Chain	Residue
B	THR75
B	ILE76
B	THR77
B	ASN96
B	GLU305
B	HIS308
B	GLY309
B	THR310
B	VAL311
B	THR312
B	ARG313
B	HIS314
B	ASN327

site_id	AC7
Number of Residues	19
Details	binding site for residue NAP C 501

Chain	Residue
C	LYS72
C	ALA74
C	THR75
C	ILE76
C	THR77
C	ARG82
C	ASN96
C	GLY288
C	GLU305
C	HIS308
C	GLY309
C	THR310
C	VAL311
C	THR312
C	ARG313
C	HIS314
C	THR326
C	ASN327
C	HOH607

site_id	AC8
Number of Residues	2
Details	binding site for residue CA C 502

Chain	Residue
C	ASP274
C	ASP278

site_id	AC9
Number of Residues	7
Details	binding site for residue GOL C 503

Chain	Residue
C	GLN138
C	ASP219
C	LYS269
C	TYR271
C	ASP272
D	GLN138
D	TYR271

site_id	AD1
Number of Residues	2
Details	binding site for residue GOL C 504

Chain	Residue
C	LYS27
C	TYR41

site_id	AD2
Number of Residues	21
Details	binding site for residue NAP D 501

Chain	Residue
C	LEU249
C	ASP252
C	ARG259
D	LYS72
D	ALA74
D	THR75
D	THR77
D	ARG82
D	ASN96
D	LEU287
D	GLU305
D	HIS308
D	GLY309
D	THR310
D	VAL311
D	THR312
D	ARG313
D	HIS314
D	THR326
D	ASN327
D	AKG503

site_id	AD3
Number of Residues	4
Details	binding site for residue CA D 502

Chain	Residue
D	ARG109
D	ASP274
D	ASP278
D	AKG503

site_id	AD4
Number of Residues	12
Details	binding site for residue AKG D 503

Chain	Residue
D	ASP274
D	ALA307
D	NAP501
D	CA502
C	LYS211
C	ILE214
D	THR77
D	SER94
D	ASN96
D	ARG100
D	ARG109
D	ARG132

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NYDGDVqSDslAqgf.GSLGM

Chain	Residue	Details
A	ASN270-MET289

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)