5Y52

Crystal Structure of Highly Active BTUO Mutant P287G Improved by Humidity Control at 83% RH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004846	molecular_function	urate oxidase activity
A	0006144	biological_process	purine nucleobase metabolic process
B	0004846	molecular_function	urate oxidase activity
B	0006144	biological_process	purine nucleobase metabolic process
C	0004846	molecular_function	urate oxidase activity
C	0006144	biological_process	purine nucleobase metabolic process
D	0004846	molecular_function	urate oxidase activity
D	0006144	biological_process	purine nucleobase metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue AZA A 401

Chain	Residue
A	PHE184
B	ALA72
B	THR73
A	LEU195
A	ARG201
A	SER248
A	ILE249
A	GLN250
A	ASN276
A	OXY402
A	HOH673

---

site_id	AC2
Number of Residues	5
Details	binding site for residue OXY A 402

Chain	Residue
A	ASN276
A	GLY302
A	GLN304
A	AZA401
B	THR73

---

site_id	AC3
Number of Residues	2
Details	binding site for residue SO4 A 403

Chain	Residue
A	ARG298
C	ARG298

---

site_id	AC4
Number of Residues	4
Details	binding site for residue EDO A 404

Chain	Residue
A	GLU119
A	ALA148
A	GLU231
A	ARG234

---

site_id	AC5
Number of Residues	12
Details	binding site for residue AZA B 401

Chain	Residue
A	VAL70
A	ALA72
A	THR73
B	PHE184
B	LEU195
B	ARG201
B	SER248
B	ILE249
B	GLN250
B	ASN276
B	OXY402
B	HOH669

---

site_id	AC6
Number of Residues	5
Details	binding site for residue OXY B 402

Chain	Residue
A	THR73
B	ASN276
B	GLY302
B	GLN304
B	AZA401

---

site_id	AC7
Number of Residues	4
Details	binding site for residue EDO B 403

Chain	Residue
B	GLU119
B	GLU231
B	ARG234
B	EDO406

---

site_id	AC8
Number of Residues	3
Details	binding site for residue EDO B 404

Chain	Residue
B	GLN275
B	TYR301
B	PHE303

---

site_id	AC9
Number of Residues	7
Details	binding site for residue EDO B 405

Chain	Residue
B	HIS42
B	ILE43
B	LEU44
B	PHE122
B	PHE140
B	HOH582
B	HOH704

---

site_id	AD1
Number of Residues	6
Details	binding site for residue EDO B 406

Chain	Residue
B	GLU123
B	SER143
B	ASN145
B	GLU146
B	EDO403
B	HOH586

---

site_id	AD2
Number of Residues	11
Details	binding site for residue AZA C 401

Chain	Residue
C	PHE184
C	LEU195
C	ARG201
C	SER248
C	ILE249
C	GLN250
C	ASN276
C	OXY402
C	HOH570
D	ALA72
D	THR73

---

site_id	AD3
Number of Residues	5
Details	binding site for residue OXY C 402

Chain	Residue
C	ASN276
C	GLY302
C	GLN304
C	AZA401
D	THR73

---

site_id	AD4
Number of Residues	4
Details	binding site for residue EDO C 403

Chain	Residue
C	GLU119
C	GLU231
C	ARG234
C	EDO404

---

site_id	AD5
Number of Residues	6
Details	binding site for residue EDO C 404

Chain	Residue
C	GLU123
C	SER143
C	ASN145
C	GLU146
C	EDO403
C	HOH522

---

site_id	AD6
Number of Residues	7
Details	binding site for residue EDO C 405

Chain	Residue
C	HIS42
C	ILE43
C	LEU44
C	PHE122
C	PHE140
C	HOH532
C	HOH678

---

site_id	AD7
Number of Residues	4
Details	binding site for residue EDO C 406

Chain	Residue
C	LYS50
C	HOH615
C	HOH756
D	TYR301

---

site_id	AD8
Number of Residues	7
Details	binding site for residue EDO D 401

Chain	Residue
D	PHE122
D	PHE140
D	HOH550
D	HOH603
D	HIS42
D	ILE43
D	LEU44

---

site_id	AD9
Number of Residues	11
Details	binding site for residue AZA D 402

Chain	Residue
C	ALA72
C	THR73
D	PHE184
D	LEU195
D	ARG201
D	SER248
D	ILE249
D	GLN250
D	ASN276
D	OXY403
D	HOH615

---

site_id	AE1
Number of Residues	5
Details	binding site for residue OXY D 403

Chain	Residue
C	THR73
D	ASN276
D	GLY302
D	GLN304
D	AZA402

---

site_id	AE2
Number of Residues	2
Details	binding site for residue SO4 D 404

Chain	Residue
B	ARG298
D	ARG298

---

site_id	AE3
Number of Residues	3
Details	binding site for residue EDO D 405

Chain	Residue
D	GLU119
D	GLU231
D	ARG234

---

Functional Information from PROSITE/UniProt

site_id	PS00366
Number of Residues	28
Details	URICASE Uricase signature. LqLIKVSgNsFvgFirdeYttLpedsnR

Chain	Residue	Details
A	LEU174-ARG201

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Charge relay system; for urate oxidase activity","evidences":[{"source":"UniProtKB","id":"D0VWQ1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"UniProtKB","id":"Q00511","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q00511","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"FEB-2009","submissionDatabase":"PDB data bank","title":"Crystal structure of urate oxidase from Bacillus sp.","authoringGroup":["Mycobacterium tuberculosis structural genomics consortium (TB)"]}}]}

Chain

Residue

Details

---