5XLH
Crystal structure of aerobically purified and aerobically crystallized for 12weeks D. vulgaris Miyazaki F [NiFe]-hydrogenase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| L | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| L | 0016151 | molecular_function | nickel cation binding |
| L | 0016491 | molecular_function | oxidoreductase activity |
| L | 0042597 | cellular_component | periplasmic space |
| L | 0046872 | molecular_function | metal ion binding |
| L | 0047806 | molecular_function | cytochrome-c3 hydrogenase activity |
| S | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| S | 0009055 | molecular_function | electron transfer activity |
| S | 0009061 | biological_process | anaerobic respiration |
| S | 0009375 | cellular_component | ferredoxin hydrogenase complex |
| S | 0016020 | cellular_component | membrane |
| S | 0016491 | molecular_function | oxidoreductase activity |
| S | 0042597 | cellular_component | periplasmic space |
| S | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| S | 0046872 | molecular_function | metal ion binding |
| S | 0047806 | molecular_function | cytochrome-c3 hydrogenase activity |
| S | 0051536 | molecular_function | iron-sulfur cluster binding |
| S | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| S | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue SF4 S 1001 |
| Chain | Residue |
| L | ARG79 |
| S | CYS17 |
| S | CYS20 |
| S | THR113 |
| S | CYS114 |
| S | GLY149 |
| S | CYS150 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue SF4 S 1002 |
| Chain | Residue |
| S | LEU194 |
| S | CYS216 |
| S | LEU217 |
| S | CYS222 |
| S | HIS188 |
| S | CYS191 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | binding site for residue F3S S 1003 |
| Chain | Residue |
| L | GLN237 |
| S | ASN229 |
| S | CYS231 |
| S | PHE236 |
| S | TRP241 |
| S | CYS249 |
| S | ILE250 |
| S | CYS252 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue MPD S 1004 |
| Chain | Residue |
| L | PHE320 |
| L | LYS321 |
| L | MET379 |
| S | LEU194 |
| S | PRO195 |
| S | ASP198 |
| S | HOH1255 |
| site_id | AC5 |
| Number of Residues | 12 |
| Details | binding site for residue NFV L 601 |
| Chain | Residue |
| L | CYS81 |
| L | CYS84 |
| L | HIS88 |
| L | ALA477 |
| L | PRO478 |
| L | ARG479 |
| L | LEU482 |
| L | VAL500 |
| L | PRO501 |
| L | SER502 |
| L | CSO546 |
| L | CYS549 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue MG L 602 |
| Chain | Residue |
| L | GLU62 |
| L | LEU498 |
| L | HIS552 |
| L | HOH740 |
| L | HOH772 |
| L | HOH811 |
Functional Information from PROSITE/UniProt
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilkgrdprdaqhftQRtCGVC |
| Chain | Residue | Details |
| L | ARG59-CYS84 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCIACgv.H |
| Chain | Residue | Details |
| L | PHE543-HIS552 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10378274, ECO:0000269|PubMed:9438867, ECO:0007744|PDB:1H2A, ECO:0007744|PDB:1H2R |
| Chain | Residue | Details |
| L | GLU62 | |
| S | CYS249 | |
| S | CYS252 | |
| L | CYS81 | |
| L | CYS84 | |
| L | LEU498 | |
| L | CSO546 | |
| L | CYS549 | |
| L | HIS552 | |
| S | CYS222 | |
| S | CYS231 |
