5WQE
Crystal structure of Alicyclobacillus acidoterrestris C2c1 in complex with single-guide RNA at 3.1 Angstrom resolution
Functional Information from GO Data
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000305|PubMed:27984729, ECO:0000305|PubMed:27989439 |
| Chain | Residue | Details |
| A | ASP570 | |
| A | GLU848 | |
| A | ASP977 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27984729, ECO:0000269|PubMed:27989439 |
| Chain | Residue | Details |
| A | SER899 | |
| A | ARG911 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Binds DNA protospacer adjacent motif (PAM) on target DNA => ECO:0000269|PubMed:27984729 |
| Chain | Residue | Details |
| A | ASN400 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | SITE: Binds sgRNA => ECO:0000269|PubMed:27984729, ECO:0000269|PubMed:27989439 |
| Chain | Residue | Details |
| A | ARG415 | |
| A | GLN982 | |
| A | ARG484 | |
| A | TYR501 | |
| A | PHE600 | |
| A | HIS614 | |
| A | ARG734 | |
| A | GLN767 | |
| A | TYR825 | |
| A | GLN882 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Binds 'phosphate lock' on target strand DNA; via amide nitrogen => ECO:0000269|PubMed:27984729 |
| Chain | Residue | Details |
| A | GLY478 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | SITE: Binds 'phosphate lock' on target strand DNA => ECO:0000269|PubMed:27984729 |
| Chain | Residue | Details |
| A | ARG507 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | SITE: Disrupts base stacking adjacent to scissile phosphate => ECO:0000305|PubMed:27984729 |
| Chain | Residue | Details |
| A | TYR853 |
