5VFO

Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome

Functional Information from GO Data

Chain	GOid	namespace	contents
g	0000502	cellular_component	proteasome complex
g	0000932	cellular_component	P-body
g	0003723	molecular_function	RNA binding
g	0004175	molecular_function	endopeptidase activity
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0005654	cellular_component	nucleoplasm
g	0005737	cellular_component	cytoplasm
g	0005829	cellular_component	cytosol
g	0005839	cellular_component	proteasome core complex
g	0005840	cellular_component	ribosome
g	0006511	biological_process	ubiquitin-dependent protein catabolic process
g	0016363	cellular_component	nuclear matrix
g	0019773	cellular_component	proteasome core complex, alpha-subunit complex
g	0030016	cellular_component	myofibril
g	0030017	cellular_component	sarcomere
g	0035639	molecular_function	purine ribonucleoside triphosphate binding
g	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
g	0050727	biological_process	regulation of inflammatory response
g	0051059	molecular_function	NF-kappaB binding
g	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
g	0051603	biological_process	proteolysis involved in protein catabolic process
g	0070062	cellular_component	extracellular exosome
G	0000502	cellular_component	proteasome complex
G	0000932	cellular_component	P-body
G	0003723	molecular_function	RNA binding
G	0004175	molecular_function	endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0005840	cellular_component	ribosome
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016363	cellular_component	nuclear matrix
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030016	cellular_component	myofibril
G	0030017	cellular_component	sarcomere
G	0035639	molecular_function	purine ribonucleoside triphosphate binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0050727	biological_process	regulation of inflammatory response
G	0051059	molecular_function	NF-kappaB binding
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0070062	cellular_component	extracellular exosome
h	0000502	cellular_component	proteasome complex
h	0000932	cellular_component	P-body
h	0005515	molecular_function	protein binding
h	0005576	cellular_component	extracellular region
h	0005634	cellular_component	nucleus
h	0005654	cellular_component	nucleoplasm
h	0005737	cellular_component	cytoplasm
h	0005829	cellular_component	cytosol
h	0005839	cellular_component	proteasome core complex
h	0006511	biological_process	ubiquitin-dependent protein catabolic process
h	0009615	biological_process	response to virus
h	0019773	cellular_component	proteasome core complex, alpha-subunit complex
h	0034774	cellular_component	secretory granule lumen
h	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
h	0051603	biological_process	proteolysis involved in protein catabolic process
h	0070062	cellular_component	extracellular exosome
h	1904813	cellular_component	ficolin-1-rich granule lumen
H	0000502	cellular_component	proteasome complex
H	0000932	cellular_component	P-body
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005839	cellular_component	proteasome core complex
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0009615	biological_process	response to virus
H	0019773	cellular_component	proteasome core complex, alpha-subunit complex
H	0034774	cellular_component	secretory granule lumen
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0051603	biological_process	proteolysis involved in protein catabolic process
H	0070062	cellular_component	extracellular exosome
H	1904813	cellular_component	ficolin-1-rich granule lumen
i	0000502	cellular_component	proteasome complex
i	0000932	cellular_component	P-body
i	0005515	molecular_function	protein binding
i	0005634	cellular_component	nucleus
i	0005654	cellular_component	nucleoplasm
i	0005737	cellular_component	cytoplasm
i	0005829	cellular_component	cytosol
i	0005839	cellular_component	proteasome core complex
i	0006511	biological_process	ubiquitin-dependent protein catabolic process
i	0019773	cellular_component	proteasome core complex, alpha-subunit complex
i	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
i	0043231	cellular_component	intracellular membrane-bounded organelle
i	0051603	biological_process	proteolysis involved in protein catabolic process
i	0070062	cellular_component	extracellular exosome
I	0000502	cellular_component	proteasome complex
I	0000932	cellular_component	P-body
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0019773	cellular_component	proteasome core complex, alpha-subunit complex
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0043231	cellular_component	intracellular membrane-bounded organelle
I	0051603	biological_process	proteolysis involved in protein catabolic process
I	0070062	cellular_component	extracellular exosome
j	0000502	cellular_component	proteasome complex
j	0005515	molecular_function	protein binding
j	0005634	cellular_component	nucleus
j	0005654	cellular_component	nucleoplasm
j	0005737	cellular_component	cytoplasm
j	0005829	cellular_component	cytosol
j	0005839	cellular_component	proteasome core complex
j	0006511	biological_process	ubiquitin-dependent protein catabolic process
j	0019773	cellular_component	proteasome core complex, alpha-subunit complex
j	0042802	molecular_function	identical protein binding
j	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
j	0051603	biological_process	proteolysis involved in protein catabolic process
j	0070062	cellular_component	extracellular exosome
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0006511	biological_process	ubiquitin-dependent protein catabolic process
J	0019773	cellular_component	proteasome core complex, alpha-subunit complex
J	0042802	molecular_function	identical protein binding
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0070062	cellular_component	extracellular exosome
k	0000502	cellular_component	proteasome complex
k	0005515	molecular_function	protein binding
k	0005576	cellular_component	extracellular region
k	0005634	cellular_component	nucleus
k	0005654	cellular_component	nucleoplasm
k	0005737	cellular_component	cytoplasm
k	0005829	cellular_component	cytosol
k	0005839	cellular_component	proteasome core complex
k	0006511	biological_process	ubiquitin-dependent protein catabolic process
k	0019773	cellular_component	proteasome core complex, alpha-subunit complex
k	0034774	cellular_component	secretory granule lumen
k	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
k	0051603	biological_process	proteolysis involved in protein catabolic process
k	0070062	cellular_component	extracellular exosome
k	1904813	cellular_component	ficolin-1-rich granule lumen
K	0000502	cellular_component	proteasome complex
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
K	0019773	cellular_component	proteasome core complex, alpha-subunit complex
K	0034774	cellular_component	secretory granule lumen
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	0070062	cellular_component	extracellular exosome
K	1904813	cellular_component	ficolin-1-rich granule lumen
l	0000502	cellular_component	proteasome complex
l	0001530	molecular_function	lipopolysaccharide binding
l	0002376	biological_process	immune system process
l	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
l	0005515	molecular_function	protein binding
l	0005634	cellular_component	nucleus
l	0005654	cellular_component	nucleoplasm
l	0005737	cellular_component	cytoplasm
l	0005813	cellular_component	centrosome
l	0005829	cellular_component	cytosol
l	0005839	cellular_component	proteasome core complex
l	0006511	biological_process	ubiquitin-dependent protein catabolic process
l	0019773	cellular_component	proteasome core complex, alpha-subunit complex
l	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
l	0051603	biological_process	proteolysis involved in protein catabolic process
l	0070062	cellular_component	extracellular exosome
L	0000502	cellular_component	proteasome complex
L	0001530	molecular_function	lipopolysaccharide binding
L	0002376	biological_process	immune system process
L	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005813	cellular_component	centrosome
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0006511	biological_process	ubiquitin-dependent protein catabolic process
L	0019773	cellular_component	proteasome core complex, alpha-subunit complex
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	0070062	cellular_component	extracellular exosome
m	0000502	cellular_component	proteasome complex
m	0005515	molecular_function	protein binding
m	0005634	cellular_component	nucleus
m	0005654	cellular_component	nucleoplasm
m	0005737	cellular_component	cytoplasm
m	0005829	cellular_component	cytosol
m	0005839	cellular_component	proteasome core complex
m	0006511	biological_process	ubiquitin-dependent protein catabolic process
m	0019773	cellular_component	proteasome core complex, alpha-subunit complex
m	0031625	molecular_function	ubiquitin protein ligase binding
m	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
m	0045202	cellular_component	synapse
m	0051603	biological_process	proteolysis involved in protein catabolic process
m	0052548	biological_process	regulation of endopeptidase activity
m	0070062	cellular_component	extracellular exosome
M	0000502	cellular_component	proteasome complex
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0006511	biological_process	ubiquitin-dependent protein catabolic process
M	0019773	cellular_component	proteasome core complex, alpha-subunit complex
M	0031625	molecular_function	ubiquitin protein ligase binding
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0045202	cellular_component	synapse
M	0051603	biological_process	proteolysis involved in protein catabolic process
M	0052548	biological_process	regulation of endopeptidase activity
M	0070062	cellular_component	extracellular exosome
n	0004298	molecular_function	threonine-type endopeptidase activity
n	0005839	cellular_component	proteasome core complex
n	0051603	biological_process	proteolysis involved in protein catabolic process
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
o	0004298	molecular_function	threonine-type endopeptidase activity
o	0005839	cellular_component	proteasome core complex
o	0051603	biological_process	proteolysis involved in protein catabolic process
O	0004298	molecular_function	threonine-type endopeptidase activity
O	0005839	cellular_component	proteasome core complex
O	0051603	biological_process	proteolysis involved in protein catabolic process
p	0000502	cellular_component	proteasome complex
p	0005515	molecular_function	protein binding
p	0005634	cellular_component	nucleus
p	0005654	cellular_component	nucleoplasm
p	0005737	cellular_component	cytoplasm
p	0005829	cellular_component	cytosol
p	0005839	cellular_component	proteasome core complex
p	0019774	cellular_component	proteasome core complex, beta-subunit complex
p	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
p	0051603	biological_process	proteolysis involved in protein catabolic process
p	0070062	cellular_component	extracellular exosome
P	0000502	cellular_component	proteasome complex
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0019774	cellular_component	proteasome core complex, beta-subunit complex
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0070062	cellular_component	extracellular exosome
q	0000502	cellular_component	proteasome complex
q	0005515	molecular_function	protein binding
q	0005634	cellular_component	nucleus
q	0005654	cellular_component	nucleoplasm
q	0005737	cellular_component	cytoplasm
q	0005829	cellular_component	cytosol
q	0005839	cellular_component	proteasome core complex
q	0010498	biological_process	proteasomal protein catabolic process
q	0014070	biological_process	response to organic cyclic compound
q	0016020	cellular_component	membrane
q	0019774	cellular_component	proteasome core complex, beta-subunit complex
q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
q	0051603	biological_process	proteolysis involved in protein catabolic process
q	0070062	cellular_component	extracellular exosome
Q	0000502	cellular_component	proteasome complex
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0010498	biological_process	proteasomal protein catabolic process
Q	0014070	biological_process	response to organic cyclic compound
Q	0016020	cellular_component	membrane
Q	0019774	cellular_component	proteasome core complex, beta-subunit complex
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0070062	cellular_component	extracellular exosome
r	0004298	molecular_function	threonine-type endopeptidase activity
r	0005839	cellular_component	proteasome core complex
r	0051603	biological_process	proteolysis involved in protein catabolic process
R	0004298	molecular_function	threonine-type endopeptidase activity
R	0005839	cellular_component	proteasome core complex
R	0051603	biological_process	proteolysis involved in protein catabolic process
s	0005839	cellular_component	proteasome core complex
s	0051603	biological_process	proteolysis involved in protein catabolic process
S	0005839	cellular_component	proteasome core complex
S	0051603	biological_process	proteolysis involved in protein catabolic process
t	0005839	cellular_component	proteasome core complex
t	0051603	biological_process	proteolysis involved in protein catabolic process
T	0005839	cellular_component	proteasome core complex
T	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsaStFSPdGRvfQVEYAmkA

Chain	Residue	Details
M	TYR7-ALA29
H	TYR6-ALA28
K	TYR8-ALA30
I	TYR5-ALA27
L	TYR6-ALA28
J	TYR3-ALA25
G	PHE9-ALA31

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LAFkFrhGVIVAADsratagayiasqtvk.KvieinpyllgtmaGgaAD

Chain	Residue	Details
R	LEU4-ASP51
P	MET12-ASP59
s	LEU13-ASP60
I	LEU35-ASP82
O	ALA4-ASP51
N	MET4-ASP51
Q	ILE5-ASP52
t	LEU12-ASP59

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
T	TYR57
t	TYR57
S	SER134
s	SER34
s	SER40
s	SER134

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:O09061

Chain	Residue	Details
S	TYR122
s	TYR122
M	LYS229
m	LYS56
m	LYS205
m	LYS229

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
S	LYS176
s	LYS176
i	LYS127
i	LYS176

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
S	SER30
S	SER181
s	SER30
s	SER181
l	LYS208

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
K	SER198
k	SER198

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P17220

Chain	Residue	Details
H	TYR121
h	TYR121

---