5UTU
2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004013 | molecular_function | adenosylhomocysteinase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033353 | biological_process | S-adenosylmethionine cycle |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004013 | molecular_function | adenosylhomocysteinase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033353 | biological_process | S-adenosylmethionine cycle |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004013 | molecular_function | adenosylhomocysteinase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033353 | biological_process | S-adenosylmethionine cycle |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004013 | molecular_function | adenosylhomocysteinase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033353 | biological_process | S-adenosylmethionine cycle |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0004013 | molecular_function | adenosylhomocysteinase activity |
| E | 0005829 | cellular_component | cytosol |
| E | 0006730 | biological_process | one-carbon metabolic process |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0033353 | biological_process | S-adenosylmethionine cycle |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0004013 | molecular_function | adenosylhomocysteinase activity |
| F | 0005829 | cellular_component | cytosol |
| F | 0006730 | biological_process | one-carbon metabolic process |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0033353 | biological_process | S-adenosylmethionine cycle |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0004013 | molecular_function | adenosylhomocysteinase activity |
| G | 0005829 | cellular_component | cytosol |
| G | 0006730 | biological_process | one-carbon metabolic process |
| G | 0016787 | molecular_function | hydrolase activity |
| G | 0033353 | biological_process | S-adenosylmethionine cycle |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0004013 | molecular_function | adenosylhomocysteinase activity |
| H | 0005829 | cellular_component | cytosol |
| H | 0006730 | biological_process | one-carbon metabolic process |
| H | 0016787 | molecular_function | hydrolase activity |
| H | 0033353 | biological_process | S-adenosylmethionine cycle |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 33 |
| Details | binding site for residue NAD A 501 |
| Chain | Residue |
| A | THR212 |
| A | THR297 |
| A | GLU298 |
| A | ILE299 |
| A | ASP300 |
| A | CYS303 |
| A | THR331 |
| A | GLY332 |
| A | ASN333 |
| A | VAL336 |
| A | ILE354 |
| A | THR213 |
| A | GLY355 |
| A | HIS356 |
| A | ASN403 |
| A | HIS410 |
| A | SAH502 |
| A | HOH621 |
| A | HOH668 |
| A | HOH675 |
| A | HOH681 |
| A | HOH725 |
| A | THR214 |
| A | HOH729 |
| B | GLN476 |
| B | LYS489 |
| B | TYR493 |
| A | ASN246 |
| A | CYS250 |
| A | GLY275 |
| A | GLY277 |
| A | GLU278 |
| A | VAL279 |
| site_id | AC2 |
| Number of Residues | 21 |
| Details | binding site for residue SAH A 502 |
| Chain | Residue |
| A | LEU52 |
| A | HIS53 |
| A | THR55 |
| A | GLU57 |
| A | THR58 |
| A | CYS77 |
| A | ASN78 |
| A | ASP137 |
| A | GLU211 |
| A | THR212 |
| A | LYS241 |
| A | ASP245 |
| A | HIS356 |
| A | PHE357 |
| A | LEU401 |
| A | THR408 |
| A | HIS410 |
| A | MET415 |
| A | PHE419 |
| A | NAD501 |
| A | HOH643 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 503 |
| Chain | Residue |
| A | ARG35 |
| A | HOH752 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 504 |
| Chain | Residue |
| A | LYS2 |
| A | MET3 |
| A | GLU4 |
| A | ARG6 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 A 505 |
| Chain | Residue |
| A | ILE7 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 506 |
| Chain | Residue |
| A | LYS2 |
| A | ARG6 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 507 |
| Chain | Residue |
| A | LYS270 |
| A | ARG293 |
| C | GLN313 |
| C | HOH672 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue P33 A 508 |
| Chain | Residue |
| A | ASN481 |
| A | VAL482 |
| A | LYS489 |
| site_id | AC9 |
| Number of Residues | 34 |
| Details | binding site for residue NAD B 501 |
| Chain | Residue |
| B | HOH616 |
| B | HOH625 |
| B | HOH635 |
| B | HOH666 |
| B | HOH672 |
| A | GLN476 |
| A | LYS489 |
| A | TYR493 |
| A | HOH652 |
| B | THR212 |
| B | THR213 |
| B | THR214 |
| B | ASN246 |
| B | CYS250 |
| B | GLY275 |
| B | GLY277 |
| B | GLU278 |
| B | VAL279 |
| B | THR297 |
| B | GLU298 |
| B | ILE299 |
| B | ASP300 |
| B | CYS303 |
| B | THR331 |
| B | GLY332 |
| B | ASN333 |
| B | VAL336 |
| B | ILE354 |
| B | GLY355 |
| B | HIS356 |
| B | LEU401 |
| B | ASN403 |
| B | HIS410 |
| B | SAH502 |
| site_id | AD1 |
| Number of Residues | 20 |
| Details | binding site for residue SAH B 502 |
| Chain | Residue |
| B | HIS53 |
| B | THR55 |
| B | GLU57 |
| B | THR58 |
| B | CYS77 |
| B | ASN78 |
| B | ASP137 |
| B | GLU211 |
| B | THR212 |
| B | LYS241 |
| B | ASP245 |
| B | PHE357 |
| B | LEU401 |
| B | THR408 |
| B | GLY409 |
| B | HIS410 |
| B | MET415 |
| B | PHE419 |
| B | NAD501 |
| B | HOH601 |
| site_id | AD2 |
| Number of Residues | 2 |
| Details | binding site for residue CL B 503 |
| Chain | Residue |
| B | LYS270 |
| B | ARG293 |
| site_id | AD3 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 504 |
| Chain | Residue |
| B | HOH682 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 505 |
| Chain | Residue |
| B | MET3 |
| B | GLU4 |
| B | ARG6 |
| site_id | AD5 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 B 506 |
| Chain | Residue |
| B | TRP101 |
| site_id | AD6 |
| Number of Residues | 2 |
| Details | binding site for residue P33 B 507 |
| Chain | Residue |
| B | ASN481 |
| B | PRO487 |
| site_id | AD7 |
| Number of Residues | 28 |
| Details | binding site for residue NAD C 501 |
| Chain | Residue |
| C | THR213 |
| C | THR214 |
| C | ASN246 |
| C | GLY277 |
| C | GLU278 |
| C | VAL279 |
| C | THR297 |
| C | GLU298 |
| C | ILE299 |
| C | ASP300 |
| C | CYS303 |
| C | THR331 |
| C | GLY332 |
| C | ASN333 |
| C | VAL336 |
| C | ILE354 |
| C | GLY355 |
| C | HIS356 |
| C | LEU401 |
| C | ASN403 |
| C | HIS410 |
| C | SAH502 |
| C | HOH642 |
| C | HOH669 |
| C | HOH682 |
| D | GLN476 |
| D | LYS489 |
| D | TYR493 |
| site_id | AD8 |
| Number of Residues | 20 |
| Details | binding site for residue SAH C 502 |
| Chain | Residue |
| C | LEU52 |
| C | HIS53 |
| C | THR55 |
| C | GLU57 |
| C | THR58 |
| C | CYS77 |
| C | ASN78 |
| C | ASP137 |
| C | GLU211 |
| C | THR212 |
| C | LYS241 |
| C | ASP245 |
| C | HIS356 |
| C | PHE357 |
| C | LEU401 |
| C | THR408 |
| C | HIS410 |
| C | MET415 |
| C | NAD501 |
| C | HOH620 |
| site_id | AD9 |
| Number of Residues | 2 |
| Details | binding site for residue CL C 503 |
| Chain | Residue |
| C | GLU491 |
| D | HOH692 |
| site_id | AE1 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 C 504 |
| Chain | Residue |
| C | LYS2 |
| C | MET3 |
| C | GLU4 |
| C | ARG6 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 C 505 |
| Chain | Residue |
| A | GLN313 |
| C | LYS270 |
| C | ARG293 |
| C | HOH672 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 506 |
| Chain | Residue |
| C | ILE7 |
| C | HOH633 |
| C | HOH667 |
| site_id | AE4 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 C 507 |
| Chain | Residue |
| C | ARG343 |
| site_id | AE5 |
| Number of Residues | 30 |
| Details | binding site for residue NAD D 501 |
| Chain | Residue |
| C | GLN476 |
| C | LYS489 |
| C | TYR493 |
| D | THR212 |
| D | THR213 |
| D | THR214 |
| D | ASN246 |
| D | GLY275 |
| D | GLY277 |
| D | GLU278 |
| D | VAL279 |
| D | THR297 |
| D | GLU298 |
| D | ILE299 |
| D | ASP300 |
| D | CYS303 |
| D | THR331 |
| D | GLY332 |
| D | ASN333 |
| D | VAL336 |
| D | ILE354 |
| D | GLY355 |
| D | HIS356 |
| D | ASN403 |
| D | HIS410 |
| D | SAH502 |
| D | HOH628 |
| D | HOH636 |
| D | HOH647 |
| D | HOH664 |
| site_id | AE6 |
| Number of Residues | 22 |
| Details | binding site for residue SAH D 502 |
| Chain | Residue |
| D | LEU52 |
| D | HIS53 |
| D | THR55 |
| D | GLU57 |
| D | THR58 |
| D | ASN78 |
| D | ASP137 |
| D | GLY138 |
| D | GLU211 |
| D | THR212 |
| D | LYS241 |
| D | ASP245 |
| D | HIS356 |
| D | PHE357 |
| D | LEU401 |
| D | THR408 |
| D | GLY409 |
| D | HIS410 |
| D | MET415 |
| D | PHE419 |
| D | NAD501 |
| D | HOH615 |
| site_id | AE7 |
| Number of Residues | 1 |
| Details | binding site for residue CL D 503 |
| Chain | Residue |
| D | ARG35 |
| site_id | AE8 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 D 504 |
| Chain | Residue |
| D | ILE7 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 D 505 |
| Chain | Residue |
| D | LYS270 |
| D | ARG293 |
| D | HOH613 |
| D | HOH670 |
| site_id | AF1 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 D 506 |
| Chain | Residue |
| D | GLN83 |
| D | ASP84 |
| D | HIS85 |
| site_id | AF2 |
| Number of Residues | 4 |
| Details | binding site for residue P6G D 507 |
| Chain | Residue |
| C | LEU217 |
| D | ASN481 |
| D | VAL482 |
| D | LYS489 |
| site_id | AF3 |
| Number of Residues | 29 |
| Details | binding site for residue NAD E 501 |
| Chain | Residue |
| E | THR213 |
| E | THR214 |
| E | ASN246 |
| E | GLY277 |
| E | GLU278 |
| E | VAL279 |
| E | THR297 |
| E | GLU298 |
| E | ILE299 |
| E | ASP300 |
| E | CYS303 |
| E | THR331 |
| E | GLY332 |
| E | ASN333 |
| E | VAL336 |
| E | ILE354 |
| E | GLY355 |
| E | HIS356 |
| E | ASN403 |
| E | HIS410 |
| E | SAH502 |
| E | HOH611 |
| E | HOH621 |
| E | HOH667 |
| E | HOH690 |
| E | HOH718 |
| F | GLN476 |
| F | LYS489 |
| F | TYR493 |
| site_id | AF4 |
| Number of Residues | 20 |
| Details | binding site for residue SAH E 502 |
| Chain | Residue |
| E | LEU52 |
| E | HIS53 |
| E | THR55 |
| E | GLU57 |
| E | THR58 |
| E | CYS77 |
| E | ASN78 |
| E | ASP137 |
| E | GLU211 |
| E | THR212 |
| E | LYS241 |
| E | ASP245 |
| E | HIS356 |
| E | PHE357 |
| E | LEU401 |
| E | THR408 |
| E | HIS410 |
| E | MET415 |
| E | PHE419 |
| E | NAD501 |
| site_id | AF5 |
| Number of Residues | 1 |
| Details | binding site for residue CL E 503 |
| Chain | Residue |
| E | ARG343 |
| site_id | AF6 |
| Number of Residues | 1 |
| Details | binding site for residue CL E 504 |
| Chain | Residue |
| E | ARG35 |
| site_id | AF7 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 E 505 |
| Chain | Residue |
| E | LYS2 |
| E | MET3 |
| E | GLU4 |
| E | ARG6 |
| site_id | AF8 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 E 506 |
| Chain | Residue |
| E | LYS270 |
| E | ARG293 |
| E | HOH613 |
| E | HOH680 |
| E | HOH717 |
| G | GLN313 |
| site_id | AF9 |
| Number of Residues | 34 |
| Details | binding site for residue NAD F 501 |
| Chain | Residue |
| E | GLN476 |
| E | LYS489 |
| E | TYR493 |
| F | THR212 |
| F | THR213 |
| F | THR214 |
| F | ASN246 |
| F | GLY275 |
| F | GLY277 |
| F | GLU278 |
| F | VAL279 |
| F | THR297 |
| F | GLU298 |
| F | ILE299 |
| F | ASP300 |
| F | CYS303 |
| F | ALA330 |
| F | THR331 |
| F | GLY332 |
| F | ASN333 |
| F | VAL336 |
| F | ILE354 |
| F | GLY355 |
| F | HIS356 |
| F | LEU401 |
| F | ASN403 |
| F | HIS410 |
| F | SAH502 |
| F | ADN503 |
| F | HOH645 |
| F | HOH650 |
| F | HOH664 |
| F | HOH667 |
| F | HOH674 |
| site_id | AG1 |
| Number of Residues | 21 |
| Details | binding site for residue SAH F 502 |
| Chain | Residue |
| F | LEU52 |
| F | HIS53 |
| F | THR55 |
| F | GLU57 |
| F | THR58 |
| F | CYS77 |
| F | ASN78 |
| F | ASP137 |
| F | GLU211 |
| F | THR212 |
| F | LYS241 |
| F | ASP245 |
| F | HIS356 |
| F | PHE357 |
| F | LEU401 |
| F | THR408 |
| F | HIS410 |
| F | MET415 |
| F | PHE419 |
| F | NAD501 |
| F | ADN503 |
| site_id | AG2 |
| Number of Residues | 18 |
| Details | binding site for residue ADN F 503 |
| Chain | Residue |
| F | LEU52 |
| F | HIS53 |
| F | THR55 |
| F | GLU57 |
| F | THR58 |
| F | ASP137 |
| F | GLU211 |
| F | THR212 |
| F | LYS241 |
| F | ASP245 |
| F | HIS356 |
| F | LEU404 |
| F | THR408 |
| F | GLY409 |
| F | HIS410 |
| F | MET415 |
| F | NAD501 |
| F | SAH502 |
| site_id | AG3 |
| Number of Residues | 1 |
| Details | binding site for residue CL F 504 |
| Chain | Residue |
| F | TYR164 |
| site_id | AG4 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 F 506 |
| Chain | Residue |
| F | LYS270 |
| F | ARG293 |
| F | HOH621 |
| H | GLN313 |
| site_id | AG5 |
| Number of Residues | 4 |
| Details | binding site for residue P6G F 507 |
| Chain | Residue |
| E | LEU217 |
| F | ASN481 |
| F | PRO487 |
| F | LYS489 |
| site_id | AG6 |
| Number of Residues | 31 |
| Details | binding site for residue NAD G 501 |
| Chain | Residue |
| G | THR213 |
| G | THR214 |
| G | ASN246 |
| G | CYS250 |
| G | GLY275 |
| G | GLY277 |
| G | GLU278 |
| G | VAL279 |
| G | THR297 |
| G | GLU298 |
| G | ILE299 |
| G | ASP300 |
| G | CYS303 |
| G | THR331 |
| G | GLY332 |
| G | ASN333 |
| G | VAL336 |
| G | ILE354 |
| G | GLY355 |
| G | HIS356 |
| G | LEU401 |
| G | ASN403 |
| G | HIS410 |
| G | SAH502 |
| G | HOH647 |
| G | HOH653 |
| G | HOH670 |
| G | HOH681 |
| H | GLN476 |
| H | LYS489 |
| H | TYR493 |
| site_id | AG7 |
| Number of Residues | 21 |
| Details | binding site for residue SAH G 502 |
| Chain | Residue |
| G | LEU52 |
| G | HIS53 |
| G | THR55 |
| G | GLU57 |
| G | THR58 |
| G | CYS77 |
| G | ASN78 |
| G | ASP137 |
| G | GLU211 |
| G | THR212 |
| G | LYS241 |
| G | ASP245 |
| G | HIS356 |
| G | PHE357 |
| G | LEU401 |
| G | THR408 |
| G | HIS410 |
| G | MET415 |
| G | PHE419 |
| G | NAD501 |
| G | HOH707 |
| site_id | AG8 |
| Number of Residues | 1 |
| Details | binding site for residue CL G 503 |
| Chain | Residue |
| G | ARG35 |
| site_id | AG9 |
| Number of Residues | 2 |
| Details | binding site for residue CL G 504 |
| Chain | Residue |
| G | LYS2 |
| G | ARG6 |
| site_id | AH1 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 G 505 |
| Chain | Residue |
| G | LYS2 |
| G | MET3 |
| G | GLU4 |
| G | ARG6 |
| site_id | AH2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 G 506 |
| Chain | Residue |
| E | GLN313 |
| G | LYS270 |
| G | ARG293 |
| G | HOH680 |
| site_id | AH3 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 G 507 |
| Chain | Residue |
| G | LYS8 |
| site_id | AH4 |
| Number of Residues | 3 |
| Details | binding site for residue PEG G 508 |
| Chain | Residue |
| G | ASN481 |
| G | VAL482 |
| G | PRO487 |
| site_id | AH5 |
| Number of Residues | 2 |
| Details | binding site for residue PEG G 509 |
| Chain | Residue |
| G | ASN481 |
| G | SER483 |
| site_id | AH6 |
| Number of Residues | 32 |
| Details | binding site for residue NAD H 501 |
| Chain | Residue |
| G | GLN476 |
| G | LYS489 |
| G | TYR493 |
| H | THR212 |
| H | THR213 |
| H | THR214 |
| H | ASN246 |
| H | GLY275 |
| H | GLY277 |
| H | GLU278 |
| H | VAL279 |
| H | THR297 |
| H | GLU298 |
| H | ILE299 |
| H | ASP300 |
| H | CYS303 |
| H | THR331 |
| H | GLY332 |
| H | ASN333 |
| H | VAL336 |
| H | ILE354 |
| H | GLY355 |
| H | HIS356 |
| H | ASN403 |
| H | HIS410 |
| H | SAH502 |
| H | ADN503 |
| H | HOH626 |
| H | HOH636 |
| H | HOH640 |
| H | HOH653 |
| H | HOH678 |
| site_id | AH7 |
| Number of Residues | 21 |
| Details | binding site for residue SAH H 502 |
| Chain | Residue |
| H | LEU52 |
| H | HIS53 |
| H | THR55 |
| H | GLU57 |
| H | THR58 |
| H | CYS77 |
| H | ASN78 |
| H | ASP137 |
| H | GLU211 |
| H | THR212 |
| H | LYS241 |
| H | ASP245 |
| H | HIS356 |
| H | PHE357 |
| H | LEU401 |
| H | THR408 |
| H | HIS410 |
| H | MET415 |
| H | NAD501 |
| H | ADN503 |
| H | HOH608 |
| site_id | AH8 |
| Number of Residues | 17 |
| Details | binding site for residue ADN H 503 |
| Chain | Residue |
| H | HIS53 |
| H | THR55 |
| H | GLU57 |
| H | THR58 |
| H | ASP137 |
| H | GLU211 |
| H | THR212 |
| H | LYS241 |
| H | ASP245 |
| H | HIS356 |
| H | THR408 |
| H | GLY409 |
| H | HIS410 |
| H | MET415 |
| H | PHE419 |
| H | NAD501 |
| H | SAH502 |
| site_id | AH9 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 H 506 |
| Chain | Residue |
| F | GLN313 |
| H | LYS270 |
| H | ARG293 |
| H | HOH644 |
| site_id | AI1 |
| Number of Residues | 3 |
| Details | binding site for residue P33 H 507 |
| Chain | Residue |
| G | LEU217 |
| H | ASN481 |
| H | PRO487 |
Functional Information from PROSITE/UniProt
| site_id | PS00738 |
| Number of Residues | 15 |
| Details | ADOHCYASE_1 S-adenosyl-L-homocysteine hydrolase signature 1. SCNiYSTQDhAAAAL |
| Chain | Residue | Details |
| A | SER76-LEU90 |
| site_id | PS00739 |
| Number of Residues | 17 |
| Details | ADOHCYASE_2 S-adenosyl-L-homocysteine hydrolase signature 2. GKkivVlGYGeVGKGc.A |
| Chain | Residue | Details |
| A | GLY268-ALA284 |
