5ULP
Structure of the NS5 methyltransferase from Zika bound to MS2042
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| A | 0004483 | molecular_function | methyltransferase cap1 activity |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0032259 | biological_process | methylation |
| B | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| B | 0004483 | molecular_function | methyltransferase cap1 activity |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 27 |
| Details | binding site for residue KB1 A 301 |
| Chain | Residue |
| A | SER56 |
| A | HIS110 |
| A | VAL130 |
| A | ASP131 |
| A | VAL132 |
| A | PHE133 |
| A | ASP146 |
| A | GLY148 |
| A | LYS182 |
| A | CL304 |
| A | IPA308 |
| A | GLY58 |
| A | HOH428 |
| A | HOH460 |
| A | HOH475 |
| A | HOH545 |
| A | HOH558 |
| A | HOH561 |
| A | HOH567 |
| A | HOH591 |
| A | GLY81 |
| A | CYS82 |
| A | GLY83 |
| A | GLY86 |
| A | TRP87 |
| A | THR104 |
| A | LYS105 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 302 |
| Chain | Residue |
| A | GLY148 |
| A | GLU149 |
| A | HOH513 |
| A | HOH575 |
| A | HOH612 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 303 |
| Chain | Residue |
| A | ALA225 |
| A | LYS226 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 304 |
| Chain | Residue |
| A | GLY148 |
| A | KB1301 |
| A | HOH612 |
| A | HOH654 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue URE A 305 |
| Chain | Residue |
| A | ARG37 |
| A | ARG41 |
| A | ARG57 |
| A | ARG84 |
| A | HOH402 |
| A | HOH517 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue URE A 306 |
| Chain | Residue |
| A | LEU71 |
| A | GLN72 |
| A | ALA178 |
| A | SER223 |
| A | GLY224 |
| A | HOH452 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue URE A 307 |
| Chain | Residue |
| A | LEU162 |
| A | SER166 |
| A | ARG197 |
| A | HOH417 |
| A | HOH478 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue IPA A 308 |
| Chain | Residue |
| A | SER56 |
| A | GLY58 |
| A | LYS61 |
| A | KB1301 |
| A | HOH465 |
| A | HOH542 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 309 |
| Chain | Residue |
| A | GLY107 |
| A | HIS110 |
| A | GLU112 |
| A | HOH599 |
| A | HOH610 |
| site_id | AD1 |
| Number of Residues | 25 |
| Details | binding site for residue KB1 B 301 |
| Chain | Residue |
| B | SER56 |
| B | GLY58 |
| B | GLY81 |
| B | CYS82 |
| B | GLY83 |
| B | GLY86 |
| B | TRP87 |
| B | THR104 |
| B | LYS105 |
| B | VAL130 |
| B | ASP131 |
| B | VAL132 |
| B | PHE133 |
| B | ASP146 |
| B | GLY148 |
| B | LYS182 |
| B | NA308 |
| B | HOH446 |
| B | HOH450 |
| B | HOH471 |
| B | HOH478 |
| B | HOH561 |
| B | HOH566 |
| B | HOH584 |
| B | HOH587 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue CL B 302 |
| Chain | Residue |
| B | GLY148 |
| B | GLU149 |
| B | HOH601 |
| B | HOH628 |
| site_id | AD3 |
| Number of Residues | 3 |
| Details | binding site for residue URE B 303 |
| Chain | Residue |
| B | MET114 |
| B | LEU115 |
| B | HOH462 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue URE B 304 |
| Chain | Residue |
| B | HOH401 |
| B | HOH531 |
| B | SER166 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue URE B 305 |
| Chain | Residue |
| B | LEU71 |
| B | GLN72 |
| B | ALA178 |
| B | SER223 |
| B | GLY224 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue URE B 306 |
| Chain | Residue |
| B | ARG37 |
| B | ARG41 |
| B | VAL55 |
| B | SER56 |
| B | ARG84 |
| B | HOH553 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue URE B 307 |
| Chain | Residue |
| B | GLU67 |
| B | GLU254 |
| B | GLU255 |
| B | HOH432 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue NA B 308 |
| Chain | Residue |
| B | GLY148 |
| B | KB1301 |
| B | HOH628 |
| B | HOH691 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | Region: {"description":"SUMO-interacting motif (SIM)","evidences":[{"source":"PubMed","id":"32699085","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Active site: {"description":"For 2'-O-MTase activity","evidences":[{"source":"UniProtKB","id":"Q6YMS4","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 28 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"27866982","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"27633330","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27866982","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28031359","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 14 |
| Details | Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Site: {"description":"mRNA cap binding; via carbonyl oxygen","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | Site: {"description":"Essential for 2'-O-methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Site: {"description":"Essential for 2'-O-methyltransferase and N-7 methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P03314","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
