5UIP
structure of DHFR with bound DAP, p-ABG and NADP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046656 | biological_process | folic acid biosynthetic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005542 | molecular_function | folic acid binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0031427 | biological_process | response to methotrexate |
| B | 0044281 | biological_process | small molecule metabolic process |
| B | 0046452 | biological_process | dihydrofolate metabolic process |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0046656 | biological_process | folic acid biosynthetic process |
| B | 0046677 | biological_process | response to antibiotic |
| B | 0050661 | molecular_function | NADP binding |
| B | 0051870 | molecular_function | methotrexate binding |
| B | 0051871 | molecular_function | dihydrofolic acid binding |
| B | 0070401 | molecular_function | NADP+ binding |
| B | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue 8DM A 201 |
| Chain | Residue |
| A | PHE31 |
| A | LYS32 |
| A | ARG52 |
| A | ARG57 |
| A | HOH322 |
| A | HOH386 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue LG3 A 202 |
| Chain | Residue |
| A | PHE31 |
| A | ILE94 |
| A | TYR100 |
| A | THR113 |
| A | ILE5 |
| A | ALA6 |
| A | ASP27 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | binding site for residue NAP A 203 |
| Chain | Residue |
| A | GLY43 |
| A | ARG44 |
| A | HIS45 |
| A | THR46 |
| A | LEU62 |
| A | SER63 |
| A | SER64 |
| A | LYS76 |
| A | GLY96 |
| A | GLY97 |
| A | ARG98 |
| A | VAL99 |
| A | GLN102 |
| A | PHE137 |
| A | HOH312 |
| A | HOH339 |
| A | HOH352 |
| A | HOH381 |
| A | HOH387 |
| A | HOH412 |
| A | HOH428 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 204 |
| Chain | Residue |
| A | HOH330 |
| A | HOH347 |
| A | HOH406 |
| B | HOH327 |
| B | HOH343 |
| B | HOH448 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue 8DM B 201 |
| Chain | Residue |
| B | PHE31 |
| B | ARG52 |
| B | ARG57 |
| B | LG3202 |
| B | HOH302 |
| B | HOH374 |
| B | HOH383 |
| B | HOH409 |
| B | HOH437 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | binding site for residue LG3 B 202 |
| Chain | Residue |
| B | ILE5 |
| B | ALA6 |
| B | ASP27 |
| B | PHE31 |
| B | ILE94 |
| B | TYR100 |
| B | THR113 |
| B | 8DM201 |
| B | HOH336 |
| site_id | AC7 |
| Number of Residues | 29 |
| Details | binding site for residue NAP B 203 |
| Chain | Residue |
| A | ASP70 |
| A | VAL72 |
| A | THR73 |
| A | HOH311 |
| B | MET16 |
| B | GLU17 |
| B | GLY43 |
| B | ARG44 |
| B | HIS45 |
| B | THR46 |
| B | LEU62 |
| B | SER63 |
| B | SER64 |
| B | LYS76 |
| B | GLY96 |
| B | GLY97 |
| B | ARG98 |
| B | VAL99 |
| B | GLN102 |
| B | ASP122 |
| B | THR123 |
| B | PHE137 |
| B | HOH346 |
| B | HOH347 |
| B | HOH350 |
| B | HOH358 |
| B | HOH363 |
| B | HOH419 |
| B | HOH440 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue NA B 204 |
| Chain | Residue |
| A | HOH340 |
| A | HOH355 |
| A | HOH473 |
| B | HOH324 |
| B | HOH349 |
| B | HOH357 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:9012674 |
| Chain | Residue | Details |
| A | ILE5 | |
| B | THR113 | |
| A | ASP27 | |
| A | ARG52 | |
| A | ARG57 | |
| A | THR113 | |
| B | ILE5 | |
| B | ASP27 | |
| B | ARG52 | |
| B | ARG57 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:19374017 |
| Chain | Residue | Details |
| A | ALA7 | |
| B | SER63 | |
| B | LYS76 | |
| B | GLY95 | |
| A | VAL13 | |
| A | HIS45 | |
| A | SER63 | |
| A | LYS76 | |
| A | GLY95 | |
| B | ALA7 | |
| B | VAL13 | |
| B | HIS45 |
