5TCJ
Crystal structure of tryptophan synthase from M. tuberculosis - aminoacrylate and BRD4592-bound form
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000162 | biological_process | L-tryptophan biosynthetic process |
| A | 0004834 | molecular_function | tryptophan synthase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006568 | biological_process | L-tryptophan metabolic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| B | 0000162 | biological_process | L-tryptophan biosynthetic process |
| B | 0004834 | molecular_function | tryptophan synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006568 | biological_process | L-tryptophan metabolic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 1901605 | biological_process | alpha-amino acid metabolic process |
| C | 0000162 | biological_process | L-tryptophan biosynthetic process |
| C | 0004834 | molecular_function | tryptophan synthase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006568 | biological_process | L-tryptophan metabolic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| C | 0016829 | molecular_function | lyase activity |
| D | 0000162 | biological_process | L-tryptophan biosynthetic process |
| D | 0004834 | molecular_function | tryptophan synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006568 | biological_process | L-tryptophan metabolic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 1901605 | biological_process | alpha-amino acid metabolic process |
| E | 0000162 | biological_process | L-tryptophan biosynthetic process |
| E | 0004834 | molecular_function | tryptophan synthase activity |
| E | 0005829 | cellular_component | cytosol |
| E | 0005886 | cellular_component | plasma membrane |
| E | 0006568 | biological_process | L-tryptophan metabolic process |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| E | 0016829 | molecular_function | lyase activity |
| F | 0000162 | biological_process | L-tryptophan biosynthetic process |
| F | 0004834 | molecular_function | tryptophan synthase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006568 | biological_process | L-tryptophan metabolic process |
| F | 0008652 | biological_process | amino acid biosynthetic process |
| F | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| F | 0016829 | molecular_function | lyase activity |
| F | 1901605 | biological_process | alpha-amino acid metabolic process |
| G | 0000162 | biological_process | L-tryptophan biosynthetic process |
| G | 0004834 | molecular_function | tryptophan synthase activity |
| G | 0005829 | cellular_component | cytosol |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0006568 | biological_process | L-tryptophan metabolic process |
| G | 0008652 | biological_process | amino acid biosynthetic process |
| G | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| G | 0016829 | molecular_function | lyase activity |
| H | 0000162 | biological_process | L-tryptophan biosynthetic process |
| H | 0004834 | molecular_function | tryptophan synthase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0006568 | biological_process | L-tryptophan metabolic process |
| H | 0008652 | biological_process | amino acid biosynthetic process |
| H | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| H | 0016829 | molecular_function | lyase activity |
| H | 1901605 | biological_process | alpha-amino acid metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 301 |
| Chain | Residue |
| A | TYR181 |
| A | GLY217 |
| A | LEU218 |
| A | GLY219 |
| A | VAL220 |
| A | ILE237 |
| A | GLY239 |
| A | SER240 |
| A | FMT302 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue FMT A 302 |
| Chain | Residue |
| A | TYR30 |
| A | GLU57 |
| A | TYR181 |
| A | ILE237 |
| A | MLI301 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | binding site for residue P1T B 501 |
| Chain | Residue |
| B | HIS100 |
| B | LYS101 |
| B | THR124 |
| B | GLY125 |
| B | ALA126 |
| B | GLY127 |
| B | GLN128 |
| B | HIS129 |
| B | LEU180 |
| B | THR204 |
| B | CYS244 |
| B | GLY246 |
| B | GLY247 |
| B | GLY248 |
| B | SER249 |
| B | ASN250 |
| B | GLY317 |
| B | GLU364 |
| B | SER390 |
| B | GLY391 |
| B | HOH626 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue CS B 502 |
| Chain | Residue |
| B | GLY246 |
| B | ALA282 |
| B | THR284 |
| B | TYR320 |
| B | GLY322 |
| B | HOH613 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue CS B 503 |
| Chain | Residue |
| B | GLY67 |
| B | PRO69 |
| B | HOH738 |
| B | HOH739 |
| D | GLY67 |
| D | PRO69 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue CS B 504 |
| Chain | Residue |
| B | LYS402 |
| B | TRP403 |
| H | LYS402 |
| H | TRP403 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | binding site for residue 79V B 505 |
| Chain | Residue |
| A | ASP64 |
| A | GLY66 |
| A | MET67 |
| A | ASP136 |
| B | PRO31 |
| B | LEU34 |
| B | PHE188 |
| B | PHE202 |
| B | PRO208 |
| B | HIS294 |
| B | GLY295 |
| B | HOH661 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue FMT B 506 |
| Chain | Residue |
| B | LYS47 |
| B | GLU48 |
| B | HOH658 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | binding site for residue FMT B 507 |
| Chain | Residue |
| B | ARG355 |
| D | LEU165 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue FMT B 508 |
| Chain | Residue |
| B | GLN80 |
| B | HOH709 |
| F | ARG119 |
| site_id | AD2 |
| Number of Residues | 2 |
| Details | binding site for residue FMT B 509 |
| Chain | Residue |
| B | LEU165 |
| D | ARG355 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for residue MLI G 301 |
| Chain | Residue |
| G | TYR181 |
| G | GLY217 |
| G | LEU218 |
| G | GLY219 |
| G | GLY239 |
| G | SER240 |
| G | FMT303 |
| G | HOH431 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue FMT G 302 |
| Chain | Residue |
| A | HOH418 |
| G | ARG223 |
| G | GLY258 |
| G | ALA262 |
| G | HOH447 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue FMT G 303 |
| Chain | Residue |
| G | GLU57 |
| G | TYR181 |
| G | ILE237 |
| G | MLI301 |
| site_id | AD6 |
| Number of Residues | 3 |
| Details | binding site for residue FMT G 304 |
| Chain | Residue |
| G | ASN22 |
| G | ARG24 |
| G | ARG265 |
| site_id | AD7 |
| Number of Residues | 22 |
| Details | binding site for residue P1T H 501 |
| Chain | Residue |
| H | GLN128 |
| H | HIS129 |
| H | LEU180 |
| H | THR204 |
| H | CYS244 |
| H | GLY246 |
| H | GLY247 |
| H | GLY248 |
| H | SER249 |
| H | ASN250 |
| H | GLY317 |
| H | GLU364 |
| H | SER390 |
| H | GLY391 |
| H | HOH649 |
| H | HOH698 |
| H | HIS100 |
| H | LYS101 |
| H | THR124 |
| H | GLY125 |
| H | ALA126 |
| H | GLY127 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue CS H 502 |
| Chain | Residue |
| H | GLY246 |
| H | ALA282 |
| H | THR284 |
| H | TYR320 |
| H | GLY322 |
| site_id | AD9 |
| Number of Residues | 10 |
| Details | binding site for residue 79V H 503 |
| Chain | Residue |
| G | ASP64 |
| G | MET67 |
| G | TYR108 |
| G | ASP136 |
| H | LEU34 |
| H | PHE188 |
| H | PHE202 |
| H | PRO208 |
| H | HIS294 |
| H | GLY295 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue FMT H 504 |
| Chain | Residue |
| D | ARG119 |
| D | VAL145 |
| D | HOH704 |
| H | ARG77 |
| H | GLN80 |
| H | HOH700 |
| site_id | AE2 |
| Number of Residues | 2 |
| Details | binding site for residue FMT H 505 |
| Chain | Residue |
| H | GLU48 |
| H | ARG114 |
| site_id | AE3 |
| Number of Residues | 2 |
| Details | binding site for residue FMT H 506 |
| Chain | Residue |
| H | HOH620 |
| H | HOH646 |
| site_id | AE4 |
| Number of Residues | 2 |
| Details | binding site for residue FMT H 507 |
| Chain | Residue |
| F | ARG355 |
| H | LEU165 |
| site_id | AE5 |
| Number of Residues | 2 |
| Details | binding site for residue FMT H 508 |
| Chain | Residue |
| D | ASN194 |
| H | SER84 |
| site_id | AE6 |
| Number of Residues | 6 |
| Details | binding site for residue MLI E 301 |
| Chain | Residue |
| E | TYR181 |
| E | GLY217 |
| E | LEU218 |
| E | GLY219 |
| E | GLY239 |
| E | SER240 |
| site_id | AE7 |
| Number of Residues | 20 |
| Details | binding site for residue P1T F 501 |
| Chain | Residue |
| F | HIS100 |
| F | LYS101 |
| F | THR124 |
| F | GLY125 |
| F | ALA126 |
| F | GLY127 |
| F | GLN128 |
| F | HIS129 |
| F | THR204 |
| F | CYS244 |
| F | GLY246 |
| F | GLY247 |
| F | GLY248 |
| F | SER249 |
| F | ASN250 |
| F | GLY317 |
| F | GLU364 |
| F | SER390 |
| F | GLY391 |
| F | HOH644 |
| site_id | AE8 |
| Number of Residues | 6 |
| Details | binding site for residue CS F 502 |
| Chain | Residue |
| F | GLY246 |
| F | ALA282 |
| F | THR284 |
| F | TYR320 |
| F | GLY322 |
| F | HOH616 |
| site_id | AE9 |
| Number of Residues | 2 |
| Details | binding site for residue CS F 503 |
| Chain | Residue |
| D | HOH721 |
| F | LEU142 |
| site_id | AF1 |
| Number of Residues | 6 |
| Details | binding site for residue CS F 504 |
| Chain | Residue |
| F | GLY67 |
| F | PRO69 |
| F | HOH715 |
| H | GLY67 |
| H | PRO69 |
| H | HOH736 |
| site_id | AF2 |
| Number of Residues | 10 |
| Details | binding site for residue 79V F 505 |
| Chain | Residue |
| E | ASP64 |
| E | GLY66 |
| E | ASP136 |
| F | PRO31 |
| F | LEU34 |
| F | PHE188 |
| F | TYR200 |
| F | PHE202 |
| F | PRO208 |
| F | HIS294 |
| site_id | AF3 |
| Number of Residues | 7 |
| Details | binding site for residue FMT F 506 |
| Chain | Residue |
| D | GLY141 |
| D | LEU142 |
| D | ASP143 |
| D | CS503 |
| F | GLY141 |
| F | LEU142 |
| F | ASP143 |
| site_id | AF4 |
| Number of Residues | 3 |
| Details | binding site for residue FMT F 507 |
| Chain | Residue |
| F | LYS47 |
| F | GLU48 |
| F | ARG114 |
| site_id | AF5 |
| Number of Residues | 2 |
| Details | binding site for residue FMT F 508 |
| Chain | Residue |
| E | GLN144 |
| F | SER23 |
| site_id | AF6 |
| Number of Residues | 3 |
| Details | binding site for residue FMT F 509 |
| Chain | Residue |
| F | LEU165 |
| H | ARG355 |
| H | PHE404 |
| site_id | AF7 |
| Number of Residues | 5 |
| Details | binding site for residue FMT F 510 |
| Chain | Residue |
| F | ASP274 |
| F | ARG280 |
| F | HIS312 |
| F | SER313 |
| F | ASP343 |
| site_id | AF8 |
| Number of Residues | 6 |
| Details | binding site for residue MLI C 301 |
| Chain | Residue |
| C | TYR181 |
| C | GLY217 |
| C | GLY219 |
| C | ILE237 |
| C | GLY239 |
| C | SER240 |
| site_id | AF9 |
| Number of Residues | 2 |
| Details | binding site for residue FMT C 302 |
| Chain | Residue |
| C | GLN144 |
| D | SER23 |
| site_id | AG1 |
| Number of Residues | 22 |
| Details | binding site for residue P1T D 501 |
| Chain | Residue |
| D | HIS100 |
| D | LYS101 |
| D | THR124 |
| D | GLY125 |
| D | ALA126 |
| D | GLY127 |
| D | GLN128 |
| D | HIS129 |
| D | THR204 |
| D | CYS244 |
| D | GLY246 |
| D | GLY247 |
| D | GLY248 |
| D | SER249 |
| D | ASN250 |
| D | GLY317 |
| D | LEU318 |
| D | GLU364 |
| D | SER390 |
| D | GLY391 |
| D | HOH644 |
| D | HOH658 |
| site_id | AG2 |
| Number of Residues | 7 |
| Details | binding site for residue CS D 502 |
| Chain | Residue |
| D | GLY246 |
| D | ALA282 |
| D | THR284 |
| D | TYR320 |
| D | GLY322 |
| D | HOH648 |
| D | HOH685 |
| site_id | AG3 |
| Number of Residues | 3 |
| Details | binding site for residue CS D 503 |
| Chain | Residue |
| D | LEU142 |
| F | FMT506 |
| F | HOH700 |
| site_id | AG4 |
| Number of Residues | 9 |
| Details | binding site for residue 79V D 504 |
| Chain | Residue |
| C | ASP64 |
| C | GLY66 |
| C | MET67 |
| D | LEU34 |
| D | PHE188 |
| D | PHE202 |
| D | PRO208 |
| D | HIS294 |
| D | GLY295 |
| site_id | AG5 |
| Number of Residues | 2 |
| Details | binding site for residue FMT D 505 |
| Chain | Residue |
| D | LYS47 |
| D | GLU48 |
| site_id | AG6 |
| Number of Residues | 3 |
| Details | binding site for residue FMT D 506 |
| Chain | Residue |
| D | HIS312 |
| D | SER313 |
| D | ASP319 |
| site_id | AG7 |
| Number of Residues | 2 |
| Details | binding site for residue FMT D 507 |
| Chain | Residue |
| D | ALA36 |
| D | GLU40 |
Functional Information from PROSITE/UniProt
| site_id | PS00167 |
| Number of Residues | 14 |
| Details | TRP_SYNTHASE_ALPHA Tryptophan synthase alpha chain signature. IEVGvPYSDPGMDG |
| Chain | Residue | Details |
| A | ILE56-GLY69 |
| site_id | PS00168 |
| Number of Residues | 15 |
| Details | TRP_SYNTHASE_BETA Tryptophan synthase beta chain pyridoxal-phosphate attachment site. LnHtGSHKiNnvLgQ |
| Chain | Residue | Details |
| B | LEU94-GLN108 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00131","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"HAMAP-Rule","id":"MF_00133","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
