Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5TC5

Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with butylthio-DADMe-Immucillin-A and chloride

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006139	biological_process	nucleobase-containing compound metabolic process
A	0006166	biological_process	purine ribonucleoside salvage
A	0006738	biological_process	nicotinamide riboside catabolic process
A	0009116	biological_process	nucleoside metabolic process
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
A	0019509	biological_process	L-methionine salvage from methylthioadenosine
A	0032259	biological_process	methylation
A	0033574	biological_process	response to testosterone
A	0070062	cellular_component	extracellular exosome
B	0003824	molecular_function	catalytic activity
B	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0006166	biological_process	purine ribonucleoside salvage
B	0006738	biological_process	nicotinamide riboside catabolic process
B	0009116	biological_process	nucleoside metabolic process
B	0016757	molecular_function	glycosyltransferase activity
B	0016763	molecular_function	pentosyltransferase activity
B	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
B	0019509	biological_process	L-methionine salvage from methylthioadenosine
B	0032259	biological_process	methylation
B	0033574	biological_process	response to testosterone
B	0070062	cellular_component	extracellular exosome
C	0003824	molecular_function	catalytic activity
C	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006139	biological_process	nucleobase-containing compound metabolic process
C	0006166	biological_process	purine ribonucleoside salvage
C	0006738	biological_process	nicotinamide riboside catabolic process
C	0009116	biological_process	nucleoside metabolic process
C	0016757	molecular_function	glycosyltransferase activity
C	0016763	molecular_function	pentosyltransferase activity
C	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
C	0019509	biological_process	L-methionine salvage from methylthioadenosine
C	0032259	biological_process	methylation
C	0033574	biological_process	response to testosterone
C	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue BIG A 301

Chain	Residue
A	PRO69
A	ASP220
A	ASP222
A	CL302
A	HOH411
A	HOH430
C	HIS137
C	LEU279
A	ALA94
A	CYS95
A	GLY96
A	PHE177
A	ILE194
A	ASN195
A	MET196
A	THR219

site_id	AC2
Number of Residues	4
Details	binding site for residue CL A 302

Chain	Residue
A	THR93
A	MET196
A	THR197
A	BIG301

site_id	AC3
Number of Residues	4
Details	binding site for residue CL A 303

Chain	Residue
A	ARG116
B	ARG116
C	ARG116
C	HOH470

site_id	AC4
Number of Residues	15
Details	binding site for residue BIG B 301

Chain	Residue
A	HIS137
A	HOH437
B	PRO69
B	ALA94
B	CYS95
B	GLY96
B	PHE177
B	ASN195
B	MET196
B	THR219
B	ASP220
B	ASP222
B	CL302
B	HOH412
B	HOH456

site_id	AC5
Number of Residues	4
Details	binding site for residue CL B 302

Chain	Residue
B	THR93
B	MET196
B	THR197
B	BIG301

site_id	AC6
Number of Residues	15
Details	binding site for residue BIG C 301

Chain	Residue
B	HIS137
B	LEU279
B	HOH420
C	PRO69
C	ALA94
C	CYS95
C	GLY96
C	ILE194
C	ASN195
C	MET196
C	THR219
C	ASP220
C	ASP222
C	CL302
C	HOH406

site_id	AC7
Number of Residues	5
Details	binding site for residue CL C 302

Chain	Residue
C	THR93
C	ASN195
C	MET196
C	THR197
C	BIG301

Functional Information from PROSITE/UniProt

site_id	PS01240
Number of Residues	41
Details	PNP_MTAP_2 Purine and other phosphorylases family 2 signature. LarhGrqHtImpskVnyqAn.IwAlkeeGcth.VIvtTAcGSL

Chain	Residue	Details
A	LEU58-LEU98

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	BINDING:

Chain	Residue	Details
B	THR18
B	ARG60
B	THR93
B	MET196
B	THR197
B	ASP220
C	THR18
C	ARG60
C	THR93
C	MET196
C	THR197
C	ASP220
A	ARG60
A	THR93
A	MET196
A	THR197
A	ASP220
A	THR18

site_id	SWS_FT_FI2
Number of Residues	6
Details	SITE: Important for substrate specificity

Chain	Residue	Details
B	VAL233
C	SER178
C	VAL233
A	SER178
A	VAL233
B	SER178

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q9CQ65

Chain	Residue	Details
A	LYS51
B	LYS51
C	LYS51

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
A	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ASP222	electrostatic stabiliser

site_id	MCSA2
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
B	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	ASP222	electrostatic stabiliser

site_id	MCSA3
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
C	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	ASP222	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)