5SDB

Crystal Structure of Human DHFR complexed with NADP and N10-formyl-tetrahydrofolate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0004146	molecular_function	dihydrofolate reductase activity
A	0005515	molecular_function	protein binding
A	0005542	molecular_function	folic acid binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006729	biological_process	tetrahydrobiopterin biosynthetic process
A	0006730	biological_process	one-carbon metabolic process
A	0008144	molecular_function	obsolete drug binding
A	0016491	molecular_function	oxidoreductase activity
A	0017148	biological_process	negative regulation of translation
A	0031103	biological_process	axon regeneration
A	0031427	biological_process	response to methotrexate
A	0046452	biological_process	dihydrofolate metabolic process
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046654	biological_process	tetrahydrofolate biosynthetic process
A	0046655	biological_process	folic acid metabolic process
A	0050661	molecular_function	NADP binding
A	0070402	molecular_function	NADPH binding
A	1990825	molecular_function	sequence-specific mRNA binding
A	2000121	biological_process	regulation of removal of superoxide radicals
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0003723	molecular_function	RNA binding
B	0003729	molecular_function	mRNA binding
B	0004146	molecular_function	dihydrofolate reductase activity
B	0005515	molecular_function	protein binding
B	0005542	molecular_function	folic acid binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006729	biological_process	tetrahydrobiopterin biosynthetic process
B	0006730	biological_process	one-carbon metabolic process
B	0008144	molecular_function	obsolete drug binding
B	0016491	molecular_function	oxidoreductase activity
B	0017148	biological_process	negative regulation of translation
B	0031103	biological_process	axon regeneration
B	0031427	biological_process	response to methotrexate
B	0046452	biological_process	dihydrofolate metabolic process
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046654	biological_process	tetrahydrofolate biosynthetic process
B	0046655	biological_process	folic acid metabolic process
B	0050661	molecular_function	NADP binding
B	0070402	molecular_function	NADPH binding
B	1990825	molecular_function	sequence-specific mRNA binding
B	2000121	biological_process	regulation of removal of superoxide radicals

Functional Information from PROSITE/UniProt

site_id	PS00075
Number of Residues	24
Details	DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfryFqrmT

Chain	Residue	Details
A	GLY16-THR39

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	362
Details	Domain: {"description":"DHFR","evidences":[{"source":"PROSITE-ProRule","id":"PRU00660","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15039552","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16222560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19478082","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"2248959","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 490

Chain	Residue	Details
A	LEU23	electrostatic stabiliser
A	GLU31	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	2
Details	M-CSA 490

Chain	Residue	Details
B	LEU23	electrostatic stabiliser
B	GLU31	electrostatic stabiliser

---