Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5P2P

X-RAY STRUCTURE OF PHOSPHOLIPASE A2 COMPLEXED WITH A SUBSTRATE-DERIVED INHIBITOR

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002446	biological_process	neutrophil mediated immunity
A	0004623	molecular_function	phospholipase A2 activity
A	0005102	molecular_function	signaling receptor binding
A	0005509	molecular_function	calcium ion binding
A	0005543	molecular_function	phospholipid binding
A	0005576	cellular_component	extracellular region
A	0006629	biological_process	lipid metabolic process
A	0006633	biological_process	fatty acid biosynthetic process
A	0006644	biological_process	phospholipid metabolic process
A	0008284	biological_process	positive regulation of cell population proliferation
A	0009986	cellular_component	cell surface
A	0010524	biological_process	positive regulation of calcium ion transport into cytosol
A	0016042	biological_process	lipid catabolic process
A	0016787	molecular_function	hydrolase activity
A	0019370	biological_process	leukotriene biosynthetic process
A	0030593	biological_process	neutrophil chemotaxis
A	0032052	molecular_function	bile acid binding
A	0032652	biological_process	regulation of interleukin-1 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0032869	biological_process	cellular response to insulin stimulus
A	0035556	biological_process	intracellular signal transduction
A	0043406	biological_process	positive regulation of MAP kinase activity
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046324	biological_process	regulation of glucose import
A	0046470	biological_process	phosphatidylcholine metabolic process
A	0046471	biological_process	phosphatidylglycerol metabolic process
A	0046872	molecular_function	metal ion binding
A	0047498	molecular_function	calcium-dependent phospholipase A2 activity
A	0048146	biological_process	positive regulation of fibroblast proliferation
A	0050482	biological_process	arachidonic acid secretion
A	0050778	biological_process	positive regulation of immune response
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	1904635	biological_process	positive regulation of podocyte apoptotic process
B	0002446	biological_process	neutrophil mediated immunity
B	0004623	molecular_function	phospholipase A2 activity
B	0005102	molecular_function	signaling receptor binding
B	0005509	molecular_function	calcium ion binding
B	0005543	molecular_function	phospholipid binding
B	0005576	cellular_component	extracellular region
B	0006629	biological_process	lipid metabolic process
B	0006633	biological_process	fatty acid biosynthetic process
B	0006644	biological_process	phospholipid metabolic process
B	0008284	biological_process	positive regulation of cell population proliferation
B	0009986	cellular_component	cell surface
B	0010524	biological_process	positive regulation of calcium ion transport into cytosol
B	0016042	biological_process	lipid catabolic process
B	0016787	molecular_function	hydrolase activity
B	0019370	biological_process	leukotriene biosynthetic process
B	0030593	biological_process	neutrophil chemotaxis
B	0032052	molecular_function	bile acid binding
B	0032652	biological_process	regulation of interleukin-1 production
B	0032757	biological_process	positive regulation of interleukin-8 production
B	0032869	biological_process	cellular response to insulin stimulus
B	0035556	biological_process	intracellular signal transduction
B	0043406	biological_process	positive regulation of MAP kinase activity
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046324	biological_process	regulation of glucose import
B	0046470	biological_process	phosphatidylcholine metabolic process
B	0046471	biological_process	phosphatidylglycerol metabolic process
B	0046872	molecular_function	metal ion binding
B	0047498	molecular_function	calcium-dependent phospholipase A2 activity
B	0048146	biological_process	positive regulation of fibroblast proliferation
B	0050482	biological_process	arachidonic acid secretion
B	0050778	biological_process	positive regulation of immune response
B	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
B	1904635	biological_process	positive regulation of podocyte apoptotic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 125

Chain	Residue
A	TYR28
A	GLY30
A	GLY32
A	ASP49
A	DHG126

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 125

Chain	Residue
B	DHG127
B	TYR28
B	GLY30
B	GLY32
B	ASP49

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 126

Chain	Residue
A	GLU71
A	SER72
A	GLU92
B	GLU71
B	SER72
B	GLU92

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE DHG A 126

Chain	Residue
A	LEU2
A	PRO18
A	PHE22
A	TYR28
A	GLY30
A	TRP31
A	GLY32
A	HIS48
A	ASP49
A	TYR52
A	TYR69
A	PHE106
A	CA125
A	HOH191
B	TRP31
B	GLY32

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE DHG B 127

Chain	Residue
A	TRP31
A	GLY32
A	HOH191
B	LEU2
B	ARG6
B	PRO18
B	TYR28
B	GLY30
B	TRP31
B	GLY32
B	CYS45
B	HIS48
B	ASP49
B	TYR52
B	ARG53
B	TYR69
B	PHE106
B	CA125
B	HOH129

Functional Information from PROSITE/UniProt

site_id	PS00118
Number of Residues	8
Details	PA2_HIS Phospholipase A2 histidine active site. CCEtHDnC

Chain	Residue	Details
A	CYS44-CYS51

site_id	PS00119
Number of Residues	11
Details	PA2_ASP Phospholipase A2 aspartic acid active site. ICNCDRNAaIC

Chain	Residue	Details
A	ILE95-CYS105

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000269\|PubMed:6876174

Chain	Residue	Details
A	HIS48
A	ILE104
B	HIS48
B	ILE104

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P00593

Chain	Residue	Details
B	GLY32
B	ASP49
A	ASP49
B	TYR28
B	GLY30
A	TYR28
A	GLY30
A	GLY32

site_id	SWS_FT_FI3
Number of Residues	2
Details	LIPID: N6-palmitoyl lysine => ECO:0000269\|PubMed:2498336

Chain	Residue	Details
A	LYS56
B	LYS56

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	8
Details	M-CSA 83

Chain	Residue	Details
A	TYR28	metal ligand
A	GLY30	metal ligand
A	GLY32	metal ligand
A	HIS48	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ASP49	metal ligand
A	TYR52	electrostatic stabiliser, hydrogen bond donor
A	SER78	electrostatic stabiliser, hydrogen bond donor
A	ILE104	electrostatic stabiliser, hydrogen bond acceptor

site_id	MCSA2
Number of Residues	8
Details	M-CSA 83

Chain	Residue	Details
B	TYR28	metal ligand
B	GLY30	metal ligand
B	GLY32	metal ligand
B	HIS48	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	ASP49	metal ligand
B	TYR52	electrostatic stabiliser, hydrogen bond donor
B	SER78	electrostatic stabiliser, hydrogen bond donor
B	ILE104	electrostatic stabiliser, hydrogen bond acceptor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)