5OPD
Structure of phosphorylated EF-Tu in complex with GTP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003746 | molecular_function | translation elongation factor activity |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006412 | biological_process | translation |
| A | 0006414 | biological_process | translational elongation |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003746 | molecular_function | translation elongation factor activity |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006412 | biological_process | translation |
| B | 0006414 | biological_process | translational elongation |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
| B | 0046677 | biological_process | response to antibiotic |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | binding site for residue IOD A 501 |
| Chain | Residue |
| A | HIS85 |
| A | GLN115 |
| site_id | AC2 |
| Number of Residues | 1 |
| Details | binding site for residue IOD A 502 |
| Chain | Residue |
| A | SER198 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue IOD A 505 |
| Chain | Residue |
| A | HIS320 |
| A | THR321 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | binding site for residue IOD A 508 |
| Chain | Residue |
| A | ALA97 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue IOD A 514 |
| Chain | Residue |
| A | ARG280 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 515 |
| Chain | Residue |
| A | HOH603 |
| A | HOH615 |
| A | HOH618 |
| A | THR26 |
| A | GTP516 |
| site_id | AC7 |
| Number of Residues | 21 |
| Details | binding site for residue GTP A 516 |
| Chain | Residue |
| A | HIS20 |
| A | VAL21 |
| A | ASP22 |
| A | HIS23 |
| A | GLY24 |
| A | LYS25 |
| A | THR26 |
| A | THR27 |
| A | PHE47 |
| A | ASP51 |
| A | ASN136 |
| A | LYS137 |
| A | ASP139 |
| A | MET140 |
| A | SER174 |
| A | ALA175 |
| A | LEU176 |
| A | MG515 |
| A | HOH603 |
| A | HOH615 |
| A | HOH649 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 519 |
| Chain | Residue |
| A | GLU118 |
| A | LEU121 |
| A | GLU308 |
| A | ASN356 |
| A | ILE357 |
| A | HOH622 |
| site_id | AC9 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 501 |
| Chain | Residue |
| B | HIS85 |
| site_id | AD1 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 503 |
| Chain | Residue |
| A | LYS283 |
| site_id | AD2 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 504 |
| Chain | Residue |
| B | SER198 |
| site_id | AD3 |
| Number of Residues | 2 |
| Details | binding site for residue IOD B 505 |
| Chain | Residue |
| B | ARG117 |
| B | HOH679 |
| site_id | AD4 |
| Number of Residues | 2 |
| Details | binding site for residue IOD B 506 |
| Chain | Residue |
| B | ARG319 |
| B | HIS320 |
| site_id | AD5 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 507 |
| Chain | Residue |
| B | PHE324 |
| site_id | AD6 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 508 |
| Chain | Residue |
| B | PHE262 |
| site_id | AD7 |
| Number of Residues | 2 |
| Details | binding site for residue IOD B 511 |
| Chain | Residue |
| B | GLY225 |
| B | THR257 |
| site_id | AD8 |
| Number of Residues | 1 |
| Details | binding site for residue IOD B 512 |
| Chain | Residue |
| B | ILE207 |
| site_id | AD9 |
| Number of Residues | 3 |
| Details | binding site for residue IOD B 513 |
| Chain | Residue |
| B | ARG234 |
| B | ARG334 |
| B | HOH654 |
| site_id | AE1 |
| Number of Residues | 2 |
| Details | binding site for residue IOD B 514 |
| Chain | Residue |
| B | ARG334 |
| B | HOH654 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue MG B 515 |
| Chain | Residue |
| B | THR26 |
| B | CYS82 |
| B | GTP516 |
| B | HOH645 |
| site_id | AE3 |
| Number of Residues | 20 |
| Details | binding site for residue GTP B 516 |
| Chain | Residue |
| A | ARG328 |
| B | VAL21 |
| B | ASP22 |
| B | HIS23 |
| B | GLY24 |
| B | LYS25 |
| B | THR26 |
| B | THR27 |
| B | ASP51 |
| B | PRO83 |
| B | ASN136 |
| B | LYS137 |
| B | ASP139 |
| B | MET140 |
| B | SER174 |
| B | ALA175 |
| B | LEU176 |
| B | MG515 |
| B | HOH611 |
| B | HOH616 |
| site_id | AE4 |
| Number of Residues | 1 |
| Details | binding site for residue NA B 517 |
| Chain | Residue |
| B | ASP267 |
| site_id | AE5 |
| Number of Residues | 1 |
| Details | binding site for residue NA B 518 |
| Chain | Residue |
| B | GLU118 |
| site_id | AE6 |
| Number of Residues | 2 |
| Details | binding site for residue NA B 519 |
| Chain | Residue |
| B | ARG289 |
| B | ASP337 |
Functional Information from PROSITE/UniProt
| site_id | PS00301 |
| Number of Residues | 16 |
| Details | G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DNapeEKaRGITIntS |
| Chain | Residue | Details |
| A | ASP51-SER66 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 14 |
| Details | Region: {"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | Region: {"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | Region: {"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Region: {"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 28 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00118","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5MI3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19150849","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24141193","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8416965","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 535 |
| Chain | Residue | Details |
| A | ASP22 | electrostatic stabiliser |
| A | LYS25 | electrostatic stabiliser |
| A | THR26 | metal ligand |
| A | HIS85 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 535 |
| Chain | Residue | Details |
| B | ASP22 | electrostatic stabiliser |
| B | LYS25 | electrostatic stabiliser |
| B | THR26 | metal ligand |
| B | HIS85 | electrostatic stabiliser |
