5OPD

Structure of phosphorylated EF-Tu in complex with GTP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0003746	molecular_function	translation elongation factor activity
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005886	cellular_component	plasma membrane
A	0006412	biological_process	translation
A	0006414	biological_process	translational elongation
A	0032045	cellular_component	guanyl-nucleotide exchange factor complex
A	0046677	biological_process	response to antibiotic
A	0097216	molecular_function	guanosine tetraphosphate binding
B	0003723	molecular_function	RNA binding
B	0003746	molecular_function	translation elongation factor activity
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005886	cellular_component	plasma membrane
B	0006412	biological_process	translation
B	0006414	biological_process	translational elongation
B	0032045	cellular_component	guanyl-nucleotide exchange factor complex
B	0046677	biological_process	response to antibiotic
B	0097216	molecular_function	guanosine tetraphosphate binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue IOD A 501

Chain	Residue
A	HIS85
A	GLN115

---

site_id	AC2
Number of Residues	1
Details	binding site for residue IOD A 502

Chain	Residue
A	SER198

---

site_id	AC3
Number of Residues	2
Details	binding site for residue IOD A 505

Chain	Residue
A	HIS320
A	THR321

---

site_id	AC4
Number of Residues	1
Details	binding site for residue IOD A 508

Chain	Residue
A	ALA97

---

site_id	AC5
Number of Residues	1
Details	binding site for residue IOD A 514

Chain	Residue
A	ARG280

---

site_id	AC6
Number of Residues	5
Details	binding site for residue MG A 515

Chain	Residue
A	HOH603
A	HOH615
A	HOH618
A	THR26
A	GTP516

---

site_id	AC7
Number of Residues	21
Details	binding site for residue GTP A 516

Chain	Residue
A	HIS20
A	VAL21
A	ASP22
A	HIS23
A	GLY24
A	LYS25
A	THR26
A	THR27
A	PHE47
A	ASP51
A	ASN136
A	LYS137
A	ASP139
A	MET140
A	SER174
A	ALA175
A	LEU176
A	MG515
A	HOH603
A	HOH615
A	HOH649

---

site_id	AC8
Number of Residues	6
Details	binding site for residue GOL A 519

Chain	Residue
A	GLU118
A	LEU121
A	GLU308
A	ASN356
A	ILE357
A	HOH622

---

site_id	AC9
Number of Residues	1
Details	binding site for residue IOD B 501

Chain	Residue
B	HIS85

---

site_id	AD1
Number of Residues	1
Details	binding site for residue IOD B 503

Chain	Residue
A	LYS283

---

site_id	AD2
Number of Residues	1
Details	binding site for residue IOD B 504

Chain	Residue
B	SER198

---

site_id	AD3
Number of Residues	2
Details	binding site for residue IOD B 505

Chain	Residue
B	ARG117
B	HOH679

---

site_id	AD4
Number of Residues	2
Details	binding site for residue IOD B 506

Chain	Residue
B	ARG319
B	HIS320

---

site_id	AD5
Number of Residues	1
Details	binding site for residue IOD B 507

Chain	Residue
B	PHE324

---

site_id	AD6
Number of Residues	1
Details	binding site for residue IOD B 508

Chain	Residue
B	PHE262

---

site_id	AD7
Number of Residues	2
Details	binding site for residue IOD B 511

Chain	Residue
B	GLY225
B	THR257

---

site_id	AD8
Number of Residues	1
Details	binding site for residue IOD B 512

Chain	Residue
B	ILE207

---

site_id	AD9
Number of Residues	3
Details	binding site for residue IOD B 513

Chain	Residue
B	ARG234
B	ARG334
B	HOH654

---

site_id	AE1
Number of Residues	2
Details	binding site for residue IOD B 514

Chain	Residue
B	ARG334
B	HOH654

---

site_id	AE2
Number of Residues	4
Details	binding site for residue MG B 515

Chain	Residue
B	THR26
B	CYS82
B	GTP516
B	HOH645

---

site_id	AE3
Number of Residues	20
Details	binding site for residue GTP B 516

Chain	Residue
A	ARG328
B	VAL21
B	ASP22
B	HIS23
B	GLY24
B	LYS25
B	THR26
B	THR27
B	ASP51
B	PRO83
B	ASN136
B	LYS137
B	ASP139
B	MET140
B	SER174
B	ALA175
B	LEU176
B	MG515
B	HOH611
B	HOH616

---

site_id	AE4
Number of Residues	1
Details	binding site for residue NA B 517

Chain	Residue
B	ASP267

---

site_id	AE5
Number of Residues	1
Details	binding site for residue NA B 518

Chain	Residue
B	GLU118

---

site_id	AE6
Number of Residues	2
Details	binding site for residue NA B 519

Chain	Residue
B	ARG289
B	ASP337

---

Functional Information from PROSITE/UniProt

site_id	PS00301
Number of Residues	16
Details	G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DNapeEKaRGITIntS

Chain	Residue	Details
A	ASP51-SER66

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
A	GLY19
A	ASP81
A	ASN136
B	GLY19
B	ASP81
B	ASN136

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylserine => ECO:0000269|PubMed:6997043, ECO:0000269|PubMed:7021545

Chain	Residue	Details
A	SER2
B	SER2

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000269|PubMed:2022614, ECO:0000269|PubMed:389663, ECO:0000269|PubMed:6997043, ECO:0000269|PubMed:7021545

Chain	Residue	Details
A	LYS57
B	LYS57

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842

Chain	Residue	Details
A	LYS314
B	LYS314

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:19150849, ECO:0000269|PubMed:24141193, ECO:0000269|PubMed:8416965

Chain	Residue	Details
A	TPO383
B	TPO383

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 535

Chain	Residue	Details
A	ASP22	electrostatic stabiliser
A	LYS25	electrostatic stabiliser
A	THR26	metal ligand
A	HIS85	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 535

Chain	Residue	Details
B	ASP22	electrostatic stabiliser
B	LYS25	electrostatic stabiliser
B	THR26	metal ligand
B	HIS85	electrostatic stabiliser

---