5OPD
Structure of phosphorylated EF-Tu in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.8369 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.840, 61.880, 65.480 |
| Unit cell angles | 70.37, 75.83, 89.41 |
Refinement procedure
| Resolution | 51.370 - 2.750 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.370 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 20752 |
| <I/σ(I)> | 9 |
| Completeness [%] | 96.6 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3550, 0.1 M Bis-Tris propane pH 6.5 and 0.2 M Sodium Iodide |
