5OHV

K33-specific affimer bound to K33 diUb

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
D	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue SO4 A 101

Chain	Residue
A	ARG42
A	ARG72
A	ARG74
C	LYS11

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site_id	AC2
Number of Residues	2
Details	binding site for residue GOL A 102

Chain	Residue
A	LYS6
D	GLU76

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site_id	AC3
Number of Residues	3
Details	binding site for residue GOL A 103

Chain	Residue
A	THR9
A	LYS11
D	GLU114

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site_id	AC4
Number of Residues	4
Details	binding site for residue SO4 C 101

Chain	Residue
A	LYS11
C	GLN31
C	ASP39
C	ARG72

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site_id	AC5
Number of Residues	3
Details	binding site for residue GOL C 102

Chain	Residue
C	THR9
C	GLY10
C	LYS11

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site_id	AC6
Number of Residues	4
Details	binding site for residue GOL B 201

Chain	Residue
B	TRP69
B	GLU76
B	TRP99
C	LYS6

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Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
A	LYS27-ASP52

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
A	ARG54
A	ARG72
C	ARG54
C	ARG72

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site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Essential for function

Chain	Residue	Details
A	HIS68
C	HIS68

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site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
A	SER65
C	SER65

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site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
A	THR66
C	THR66

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
A	GLY76
C	GLY76

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
A	LYS6
C	LYS6

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site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
A	GLY76
C	GLY76

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
A	LYS11
A	LYS48
C	LYS11
C	LYS48

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site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
A	LYS27
C	LYS27

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site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
A	LYS29
C	LYS29

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site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
A	LYS33
C	LYS33

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site_id	SWS_FT_FI12
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
A	LYS63
C	LYS63

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