5OHV

K33-specific affimer bound to K33 diUb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979490
Spacegroup name	H 3
Unit cell lengths	120.288, 120.288, 69.973
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.144 - 2.801
R-factor	0.1761
Rwork	0.174
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 4n6u
RMSD bond length	0.003
RMSD bond angle	0.776
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.144	3.936
High resolution limit [Å]	2.800	3.800
Rmerge	0.237	0.794
Number of reflections	9294
<I/σ(I)>	5	2
Completeness [%]	100.0	100
Redundancy	7	7.1
CC(1/2)		0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	295	21% PEG 3350 200 mM Li2SO4 100 mM NaOAc pH 5.2