5OHL

K6-specific affimer bound to K6 diUb

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
B	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
C	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
D	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
E	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
F	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
G	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
H	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue GOL A 201

Chain	Residue
A	ASN33
A	SER34
A	HOH307
C	TYR108

---

site_id	AC2
Number of Residues	4
Details	binding site for residue 15P B 201

Chain	Residue
B	TRP99
C	TRP69
C	TRP99
E	GLN116

---

site_id	AC3
Number of Residues	6
Details	binding site for residue 15P E 201

Chain	Residue
A	MET78
E	TRP69
E	MET78
E	TYR80
E	LYS97
A	TRP69

---

site_id	AC4
Number of Residues	6
Details	binding site for residue 15P F 201

Chain	Residue
F	TRP69
F	TRP99
G	GLN116
H	TRP69
H	MET78
H	TRP99

---

site_id	AC5
Number of Residues	3
Details	binding site for residue 15P G 201

Chain	Residue
D	TYR80
G	TRP69
G	MET78

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
I	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
I	ARG54
M	ARG72
N	ARG54
N	ARG72
O	ARG54
O	ARG72
P	ARG54
P	ARG72
I	ARG72
J	ARG54
J	ARG72
K	ARG54
K	ARG72
L	ARG54
L	ARG72
M	ARG54

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	SITE: Essential for function

Chain	Residue	Details
I	HIS68
J	HIS68
K	HIS68
L	HIS68
M	HIS68
N	HIS68
O	HIS68
P	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
I	SER65
J	SER65
K	SER65
L	SER65
M	SER65
N	SER65
O	SER65
P	SER65

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
I	THR66
J	THR66
K	THR66
L	THR66
M	THR66
N	THR66
O	THR66
P	THR66

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
I	GLY76
J	GLY76
K	GLY76
L	GLY76
M	GLY76
N	GLY76
O	GLY76
P	GLY76

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
I	LYS6
J	LYS6
K	LYS6
L	LYS6
M	LYS6
N	LYS6
O	LYS6
P	LYS6

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
M	GLY76
N	GLY76
O	GLY76
P	GLY76
I	GLY76
J	GLY76
K	GLY76
L	GLY76

---

site_id	SWS_FT_FI8
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
I	LYS11
M	LYS48
N	LYS11
N	LYS48
O	LYS11
O	LYS48
P	LYS11
P	LYS48
I	LYS48
J	LYS11
J	LYS48
K	LYS11
K	LYS48
L	LYS11
L	LYS48
M	LYS11

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
I	LYS27
J	LYS27
K	LYS27
L	LYS27
M	LYS27
N	LYS27
O	LYS27
P	LYS27

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
I	LYS29
J	LYS29
K	LYS29
L	LYS29
M	LYS29
N	LYS29
O	LYS29
P	LYS29

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
I	LYS33
J	LYS33
K	LYS33
L	LYS33
M	LYS33
N	LYS33
O	LYS33
P	LYS33

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
I	LYS63
J	LYS63
K	LYS63
L	LYS63
M	LYS63
N	LYS63
O	LYS63
P	LYS63

---