5OHL

K6-specific affimer bound to K6 diUb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	110
Detector technology	CCD
Collection date	2015-06-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.872900
Spacegroup name	P 1
Unit cell lengths	60.502, 69.672, 99.297
Unit cell angles	79.79, 79.76, 83.08

Refinement procedure

Resolution	48.230 - 2.500
R-factor	0.2015
Rwork	0.200
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 4n6u
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.230	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.260
Number of reflections	53211
<I/σ(I)>	8.2	0.5
Completeness [%]	98.4	97.5
Redundancy	1.8	1.8
CC(1/2)	0.996	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	32.5%PEG 2K MME 200mM Ammonium Acetate 0.1M Tris pH 8.5