5OHL
K6-specific affimer bound to K6 diUb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-06-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.872900 |
Spacegroup name | P 1 |
Unit cell lengths | 60.502, 69.672, 99.297 |
Unit cell angles | 79.79, 79.76, 83.08 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.230 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.260 |
Number of reflections | 53211 | |
<I/σ(I)> | 8.2 | 0.5 |
Completeness [%] | 98.4 | 97.5 |
Redundancy | 1.8 | 1.8 |
CC(1/2) | 0.996 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 32.5%PEG 2K MME 200mM Ammonium Acetate 0.1M Tris pH 8.5 |