5OCU

Molecular basis of human kinesin-8 function and inhibition

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003924	molecular_function	GTPase activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0046872	molecular_function	metal ion binding
K	0003777	molecular_function	microtubule motor activity
K	0005524	molecular_function	ATP binding
K	0007018	biological_process	microtubule-based movement
K	0008017	molecular_function	microtubule binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	binding site for residue MG K 500

Chain	Residue
K	ANP501

---

site_id	AC2
Number of Residues	14
Details	binding site for residue ANP K 501

Chain	Residue
K	THR120
K	HIS121
K	SER225
K	SER226
K	GLY261
K	MG500
K	VAL18
K	ARG19
K	ALA114
K	THR115
K	GLY116
K	ALA117
K	GLY118
K	LYS119

---

site_id	AC3
Number of Residues	1
Details	binding site for residue ZN A 501

Chain	Residue
A	HIS283

---

site_id	AC4
Number of Residues	1
Details	binding site for residue MG A 502

Chain	Residue
A	GTP503

---

site_id	AC5
Number of Residues	11
Details	binding site for residue GTP A 503

Chain	Residue
A	GLY10
A	GLN11
A	ALA12
A	ALA99
A	ALA100
A	ASN101
A	GLY143
A	GLY144
A	THR145
A	GLY146
A	MG502

---

site_id	AC6
Number of Residues	8
Details	binding site for residue GDP B 600

Chain	Residue
B	GLY10
B	GLN11
B	CYS12
B	GLY142
B	GLY143
B	GLY144
B	THR145
B	GLY146

---

site_id	AC7
Number of Residues	11
Details	binding site for residue TA1 B 601

Chain	Residue
B	VAL23
B	ASP226
B	LEU230
B	ALA233
B	PRO274
B	LEU275
B	THR276
B	ARG278
B	PRO360
B	ARG369
B	GLY370

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
B	GLY142-GLY148
A	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

site_id	PS00411
Number of Residues	12
Details	KINESIN_MOTOR_1 Kinesin motor domain signature. AKMsLIDLAGSE

Chain	Residue	Details
K	ALA252-GLU263

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	GLU254
B	SER140
B	GLY144
B	THR145
B	GLY146
B	ASN206
B	ASN228

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	GLN11
A	GLU71
A	SER140
A	GLY144
A	THR145
A	THR179
A	ASN206
A	ASN228

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Involved in polymerization => ECO:0000250

Chain	Residue	Details
A	TYR451

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	LYS40

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	SER48
A	GLY232

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:P68373

Chain	Residue	Details
A	TYR282
B	THR292

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	ARG339

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68373

Chain	Residue	Details
A	SER439

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	GLU443

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68369

Chain	Residue	Details
A	GLU445
B	LYS326

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:Q71U36

Chain	Residue	Details
A	TYR451

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
A	LYS326
A	LYS370

---