5O81

Crystal Structure of R67A/E173A Mutant of alpha-L-arabinofuranosidase Ara51 from Clostridium thermocellum

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000272	biological_process	polysaccharide catabolic process
A	0005737	cellular_component	cytoplasm
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0031222	biological_process	arabinan catabolic process
A	0046373	biological_process	L-arabinose metabolic process
A	0046556	molecular_function	alpha-L-arabinofuranosidase activity
B	0000272	biological_process	polysaccharide catabolic process
B	0005737	cellular_component	cytoplasm
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0031222	biological_process	arabinan catabolic process
B	0046373	biological_process	L-arabinose metabolic process
B	0046556	molecular_function	alpha-L-arabinofuranosidase activity
C	0000272	biological_process	polysaccharide catabolic process
C	0005737	cellular_component	cytoplasm
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0031222	biological_process	arabinan catabolic process
C	0046373	biological_process	L-arabinose metabolic process
C	0046556	molecular_function	alpha-L-arabinofuranosidase activity
D	0000272	biological_process	polysaccharide catabolic process
D	0005737	cellular_component	cytoplasm
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0031222	biological_process	arabinan catabolic process
D	0046373	biological_process	L-arabinose metabolic process
D	0046556	molecular_function	alpha-L-arabinofuranosidase activity
E	0000272	biological_process	polysaccharide catabolic process
E	0005737	cellular_component	cytoplasm
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0031222	biological_process	arabinan catabolic process
E	0046373	biological_process	L-arabinose metabolic process
E	0046556	molecular_function	alpha-L-arabinofuranosidase activity
F	0000272	biological_process	polysaccharide catabolic process
F	0005737	cellular_component	cytoplasm
F	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
F	0031222	biological_process	arabinan catabolic process
F	0046373	biological_process	L-arabinose metabolic process
F	0046556	molecular_function	alpha-L-arabinofuranosidase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:16336192

Chain	Residue	Details
A	ALA173
B	ALA173
C	ALA173
D	ALA173
E	ALA173
F	ALA173

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site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:16336192

Chain	Residue	Details
A	GLU292
B	GLU292
C	GLU292
D	GLU292
E	GLU292
F	GLU292

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site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING:

Chain	Residue	Details
E	TYR244
E	GLU292
F	GLU27
F	ASN72
F	TYR244
F	GLU292
A	GLU292
B	GLU27
B	ASN72
B	TYR244
B	GLU292
C	GLU27
C	ASN72
C	TYR244
C	GLU292
D	GLU27
D	ASN72
D	TYR244
D	GLU292
E	GLU27
E	ASN72
A	GLU27
A	ASN72
A	TYR244

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site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
D	ASN172
D	GLN352
E	ASN172
E	GLN352
F	ASN172
F	GLN352
A	ASN172
A	GLN352
B	ASN172
B	GLN352
C	ASN172
C	GLN352

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site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Important for substrate recognition => ECO:0000250

Chain	Residue	Details
B	TRP296
C	TRP296
D	TRP296
E	TRP296
F	TRP296
A	TRP296

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site_id	SWS_FT_FI6
Number of Residues	6
Details	SITE: Important for substrate recognition

Chain	Residue	Details
A	GLN352
B	GLN352
C	GLN352
D	GLN352
E	GLN352
F	GLN352

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