5O81
Crystal Structure of R67A/E173A Mutant of alpha-L-arabinofuranosidase Ara51 from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 173.070, 173.070, 271.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.667 - 2.500 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c7f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.154 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.667 | 48.667 | 2.650 |
High resolution limit [Å] | 2.500 | 7.420 | 2.500 |
Rmerge | 0.100 | 0.029 | 0.861 |
Rmeas | 0.104 | 0.031 | 0.896 |
Number of reflections | 142438 | 5861 | 22405 |
<I/σ(I)> | 21.7 | 69.55 | 2.94 |
Completeness [%] | 99.8 | 99.4 | 98.6 |
Redundancy | 13.279 | 11.916 | 13.125 |
CC(1/2) | 0.999 | 1.000 | 0.623 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1-4 dioxane, Na Cacodylate pH 6.5, sodium acetate |