Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5O4I

Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005507	molecular_function	copper ion binding
A	0005886	cellular_component	plasma membrane
A	0006826	biological_process	iron ion transport
A	0016491	molecular_function	oxidoreductase activity
A	0046872	molecular_function	metal ion binding
B	0005507	molecular_function	copper ion binding
B	0005886	cellular_component	plasma membrane
B	0006826	biological_process	iron ion transport
B	0016491	molecular_function	oxidoreductase activity
B	0046872	molecular_function	metal ion binding
C	0005507	molecular_function	copper ion binding
C	0005886	cellular_component	plasma membrane
C	0006826	biological_process	iron ion transport
C	0016491	molecular_function	oxidoreductase activity
C	0046872	molecular_function	metal ion binding
D	0005507	molecular_function	copper ion binding
D	0005886	cellular_component	plasma membrane
D	0006826	biological_process	iron ion transport
D	0016491	molecular_function	oxidoreductase activity
D	0046872	molecular_function	metal ion binding
E	0005507	molecular_function	copper ion binding
E	0005886	cellular_component	plasma membrane
E	0006826	biological_process	iron ion transport
E	0016491	molecular_function	oxidoreductase activity
E	0046872	molecular_function	metal ion binding
F	0005507	molecular_function	copper ion binding
F	0005886	cellular_component	plasma membrane
F	0006826	biological_process	iron ion transport
F	0016491	molecular_function	oxidoreductase activity
F	0046872	molecular_function	metal ion binding
G	0005507	molecular_function	copper ion binding
G	0005886	cellular_component	plasma membrane
G	0006826	biological_process	iron ion transport
G	0016491	molecular_function	oxidoreductase activity
G	0046872	molecular_function	metal ion binding
H	0005507	molecular_function	copper ion binding
H	0005886	cellular_component	plasma membrane
H	0006826	biological_process	iron ion transport
H	0016491	molecular_function	oxidoreductase activity
H	0046872	molecular_function	metal ion binding
I	0005507	molecular_function	copper ion binding
I	0005886	cellular_component	plasma membrane
I	0006826	biological_process	iron ion transport
I	0016491	molecular_function	oxidoreductase activity
I	0046872	molecular_function	metal ion binding
J	0005507	molecular_function	copper ion binding
J	0005886	cellular_component	plasma membrane
J	0006826	biological_process	iron ion transport
J	0016491	molecular_function	oxidoreductase activity
J	0046872	molecular_function	metal ion binding
K	0005507	molecular_function	copper ion binding
K	0005886	cellular_component	plasma membrane
K	0006826	biological_process	iron ion transport
K	0016491	molecular_function	oxidoreductase activity
K	0046872	molecular_function	metal ion binding
L	0005507	molecular_function	copper ion binding
L	0005886	cellular_component	plasma membrane
L	0006826	biological_process	iron ion transport
L	0016491	molecular_function	oxidoreductase activity
L	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue CU A 401

Chain	Residue
A	HIS232
A	CYS289
A	HIS294
A	MET299

site_id	AC2
Number of Residues	4
Details	binding site for residue CU A 402

Chain	Residue
A	HIS237
A	HIS288
A	HOH517
C	HIS159

site_id	AC3
Number of Residues	5
Details	binding site for residue CU A 403

Chain	Residue
A	HIS105
A	HIS157
B	HIS290
B	HOH562
A	HIS103

site_id	AC4
Number of Residues	8
Details	binding site for residue GOL A 404

Chain	Residue
A	GLY152
A	TYR153
A	SER269
A	HOH518
A	HOH556
B	GLN257
B	VAL258
B	HOH526

site_id	AC5
Number of Residues	4
Details	binding site for residue GOL A 405

Chain	Residue
A	ARG50
A	VAL52
A	ILE80
A	GLU81

site_id	AC6
Number of Residues	4
Details	binding site for residue CU B 401

Chain	Residue
B	HIS232
B	CYS289
B	HIS294
B	MET299

site_id	AC7
Number of Residues	4
Details	binding site for residue CU B 402

Chain	Residue
A	HIS159
B	HIS237
B	HIS288
B	HOH562

site_id	AC8
Number of Residues	5
Details	binding site for residue CU B 403

Chain	Residue
B	HIS103
B	HIS105
B	HIS157
C	HIS290
C	HOH523

site_id	AC9
Number of Residues	9
Details	binding site for residue GOL B 404

Chain	Residue
B	GLY152
B	TYR153
B	TRP154
B	SER269
B	HOH525
B	HOH578
C	GLN257
C	VAL258
C	HOH550

site_id	AD1
Number of Residues	9
Details	binding site for residue GOL B 405

Chain	Residue
A	LYS262
A	ASP268
A	HOH534
B	GLN257
B	ASN261
B	LYS262
B	ILE263
B	HOH518
C	GOL407

site_id	AD2
Number of Residues	9
Details	binding site for residue GOL B 406

Chain	Residue
B	LYS262
B	ASP268
B	HOH510
B	HOH548
C	GLN257
C	ASN261
C	LYS262
C	ILE263
C	GOL407

site_id	AD3
Number of Residues	8
Details	binding site for residue GOL B 407

Chain	Residue
B	GLY152
B	TYR153
B	VAL180
B	ARG181
B	ASP185
B	ARG245
B	THR246
B	HOH574

site_id	AD4
Number of Residues	2
Details	binding site for residue SO4 B 408

Chain	Residue
B	ARG147
C	GLY279

site_id	AD5
Number of Residues	5
Details	binding site for residue CU C 401

Chain	Residue
A	HIS290
A	HOH517
C	HIS103
C	HIS105
C	HIS157

site_id	AD6
Number of Residues	4
Details	binding site for residue CU C 402

Chain	Residue
C	HIS232
C	CYS289
C	HIS294
C	MET299

site_id	AD7
Number of Residues	4
Details	binding site for residue CU C 403

Chain	Residue
B	HIS159
C	HIS237
C	HIS288
C	HOH523

site_id	AD8
Number of Residues	9
Details	binding site for residue GOL C 404

Chain	Residue
A	GLN257
A	ASN261
A	LYS262
A	ILE263
C	LYS262
C	ASP268
C	GOL407
C	HOH504
C	HOH527

site_id	AD9
Number of Residues	10
Details	binding site for residue GOL C 405

Chain	Residue
C	GLY152
C	TYR153
C	TRP154
C	ARG245
C	SER269
C	HOH518
C	HOH526
C	HOH535
A	GLN257
A	VAL258

site_id	AE1
Number of Residues	9
Details	binding site for residue GOL C 406

Chain	Residue
C	GLY152
C	TYR153
C	ILE179
C	VAL180
C	ARG181
C	ASP185
C	ARG245
C	THR246
C	HOH514

site_id	AE2
Number of Residues	7
Details	binding site for residue GOL C 407

Chain	Residue
A	LYS262
A	ILE263
B	LYS262
B	GOL405
B	GOL406
C	LYS262
C	GOL404

site_id	AE3
Number of Residues	4
Details	binding site for residue CU D 401

Chain	Residue
D	HIS232
D	CYS289
D	HIS294
D	MET299

site_id	AE4
Number of Residues	4
Details	binding site for residue CU D 402

Chain	Residue
D	HIS237
D	HIS288
D	HOH574
F	HIS159

site_id	AE5
Number of Residues	5
Details	binding site for residue CU D 403

Chain	Residue
D	HIS103
D	HIS105
D	HIS157
E	HIS290
E	HOH515

site_id	AE6
Number of Residues	9
Details	binding site for residue GOL D 404

Chain	Residue
D	GLN257
D	ASN261
D	LYS262
D	ILE263
D	GOL407
D	HOH507
D	HOH518
F	LYS262
F	ASP268

site_id	AE7
Number of Residues	9
Details	binding site for residue GOL D 405

Chain	Residue
D	GLY152
D	TYR153
D	TRP154
D	SER269
D	HOH523
D	HOH554
E	GLN257
E	VAL258
E	HOH548

site_id	AE8
Number of Residues	10
Details	binding site for residue GOL D 406

Chain	Residue
D	ALA151
D	GLY152
D	TYR153
D	VAL180
D	ARG181
D	ASP185
D	ARG245
D	THR246
D	HOH503
D	HOH504

site_id	AE9
Number of Residues	7
Details	binding site for residue GOL D 407

Chain	Residue
D	LYS262
D	GOL404
E	LYS262
E	GOL404
F	LYS262
F	ILE263
F	GOL405

site_id	AF1
Number of Residues	4
Details	binding site for residue GOL D 408

Chain	Residue
D	ARG50
D	LEU79
D	ILE80
D	GLU81

site_id	AF2
Number of Residues	4
Details	binding site for residue CU E 401

Chain	Residue
E	HIS232
E	CYS289
E	HIS294
E	MET299

site_id	AF3
Number of Residues	4
Details	binding site for residue CU E 402

Chain	Residue
D	HIS159
E	HIS237
E	HIS288
E	HOH515

site_id	AF4
Number of Residues	5
Details	binding site for residue CU E 403

Chain	Residue
E	HIS103
E	HIS105
E	HIS157
E	HOH563
F	HIS290

site_id	AF5
Number of Residues	9
Details	binding site for residue GOL E 404

Chain	Residue
D	LYS262
D	ASP268
D	GOL407
D	HOH544
E	GLN257
E	ASN261
E	LYS262
E	ILE263
E	HOH513

site_id	AF6
Number of Residues	10
Details	binding site for residue GOL E 405

Chain	Residue
E	GLY152
E	TYR153
E	TRP154
E	ASN244
E	SER269
E	HOH505
E	HOH521
E	HOH540
F	GLN257
F	VAL258

site_id	AF7
Number of Residues	6
Details	binding site for residue GOL E 406

Chain	Residue
E	LYS117
E	VAL160
E	GLY162
E	THR163
E	HOH550
J	ASP97

site_id	AF8
Number of Residues	5
Details	binding site for residue GOL E 407

Chain	Residue
E	ASP97
J	LYS117
J	VAL160
J	GLY162
J	THR163

site_id	AF9
Number of Residues	8
Details	binding site for residue GOL E 408

Chain	Residue
E	GLY152
E	TYR153
E	VAL180
E	ARG181
E	ASP185
E	ARG245
E	THR246
E	HOH516

site_id	AG1
Number of Residues	4
Details	binding site for residue GOL E 409

Chain	Residue
E	ARG50
E	LEU79
E	ILE80
E	GLU81

site_id	AG2
Number of Residues	5
Details	binding site for residue CU F 401

Chain	Residue
D	HIS290
D	HOH574
F	HIS103
F	HIS105
F	HIS157

site_id	AG3
Number of Residues	4
Details	binding site for residue CU F 402

Chain	Residue
F	HIS232
F	CYS289
F	HIS294
F	MET299

site_id	AG4
Number of Residues	5
Details	binding site for residue CU F 403

Chain	Residue
E	HIS103
E	HIS159
E	HOH563
F	HIS237
F	HIS288

site_id	AG5
Number of Residues	8
Details	binding site for residue GOL F 404

Chain	Residue
D	GLN257
D	VAL258
F	GLY152
F	TYR153
F	SER269
F	HOH520
F	HOH521
F	HOH525

site_id	AG6
Number of Residues	9
Details	binding site for residue GOL F 405

Chain	Residue
D	GOL407
E	LYS262
E	ASP268
F	GLN257
F	ASN261
F	LYS262
F	ILE263
F	HOH515
F	HOH548

site_id	AG7
Number of Residues	7
Details	binding site for residue GOL F 406

Chain	Residue
F	GLY152
F	TYR153
F	VAL180
F	ARG181
F	ASP185
F	ARG245
F	THR246

site_id	AG8
Number of Residues	4
Details	binding site for residue SO4 F 407

Chain	Residue
D	GLY279
F	ARG134
F	ARG147
F	HOH537

site_id	AG9
Number of Residues	4
Details	binding site for residue CU G 401

Chain	Residue
G	HIS232
G	CYS289
G	HIS294
G	MET299

site_id	AH1
Number of Residues	5
Details	binding site for residue CU G 402

Chain	Residue
G	HIS237
G	HIS288
G	HOH557
I	HIS103
I	HIS159

site_id	AH2
Number of Residues	5
Details	binding site for residue CU G 403

Chain	Residue
G	HIS103
G	HIS105
G	HIS157
H	HIS290
H	HOH544

site_id	AH3
Number of Residues	9
Details	binding site for residue GOL G 404

Chain	Residue
G	LYS262
G	ASP268
G	GOL408
G	HOH514
G	HOH531
H	GLN257
H	ASN261
H	LYS262
H	ILE263

site_id	AH4
Number of Residues	9
Details	binding site for residue GOL G 405

Chain	Residue
G	GLY152
G	TYR153
G	TRP154
G	SER269
G	HOH503
G	HOH538
G	HOH549
H	GLN257
H	VAL258

site_id	AH5
Number of Residues	9
Details	binding site for residue GOL G 406

Chain	Residue
G	GLN257
G	ASN261
G	LYS262
G	ILE263
G	GOL408
G	HOH501
G	HOH526
I	LYS262
I	ASP268

site_id	AH6
Number of Residues	6
Details	binding site for residue GOL G 407

Chain	Residue
G	ASP243
G	GLN257
H	ASP243
H	GLN257
I	ASP243
I	GLN257

site_id	AH7
Number of Residues	8
Details	binding site for residue GOL G 408

Chain	Residue
G	LYS262
G	ILE263
G	GOL404
G	GOL406
H	LYS262
H	GOL404
I	LYS262
I	ILE263

site_id	AH8
Number of Residues	4
Details	binding site for residue SO4 G 409

Chain	Residue
G	ARG182
G	ARG245
H	ASP254
H	PRO255

site_id	AH9
Number of Residues	4
Details	binding site for residue CU H 401

Chain	Residue
H	HIS232
H	CYS289
H	HIS294
H	MET299

site_id	AI1
Number of Residues	4
Details	binding site for residue CU H 402

Chain	Residue
G	HIS159
H	HIS237
H	HIS288
H	HOH544

site_id	AI2
Number of Residues	5
Details	binding site for residue CU H 403

Chain	Residue
H	HIS103
H	HIS105
H	HIS157
I	HIS290
I	HOH526

site_id	AI3
Number of Residues	9
Details	binding site for residue GOL H 404

Chain	Residue
G	GOL408
H	LYS262
H	ASP268
H	HOH515
H	HOH525
I	GLN257
I	ASN261
I	LYS262
I	ILE263

site_id	AI4
Number of Residues	8
Details	binding site for residue GOL H 405

Chain	Residue
H	GLY152
H	TYR153
H	TRP154
H	SER269
H	HOH522
H	HOH528
I	GLN257
I	VAL258

site_id	AI5
Number of Residues	5
Details	binding site for residue CU I 401

Chain	Residue
G	HIS290
G	HOH557
I	HIS103
I	HIS105
I	HIS157

site_id	AI6
Number of Residues	4
Details	binding site for residue CU I 402

Chain	Residue
I	HIS232
I	CYS289
I	HIS294
I	MET299

site_id	AI7
Number of Residues	4
Details	binding site for residue CU I 403

Chain	Residue
H	HIS159
I	HIS237
I	HIS288
I	HOH526

site_id	AI8
Number of Residues	9
Details	binding site for residue GOL I 404

Chain	Residue
G	GLN257
G	VAL258
G	HOH525
I	GLY152
I	TYR153
I	TRP154
I	SER269
I	HOH527
I	HOH536

site_id	AI9
Number of Residues	6
Details	binding site for residue GOL I 405

Chain	Residue
C	ASP97
I	LYS117
I	VAL160
I	GLY162
I	THR163
I	HOH535

site_id	AJ1
Number of Residues	4
Details	binding site for residue GOL I 406

Chain	Residue
I	GLU137
I	GLY139
I	ARG140
I	ARG147

site_id	AJ2
Number of Residues	4
Details	binding site for residue CU J 401

Chain	Residue
J	HIS232
J	CYS289
J	HIS294
J	MET299

site_id	AJ3
Number of Residues	4
Details	binding site for residue CU J 402

Chain	Residue
J	HIS237
J	HIS288
J	HOH553
L	HIS159

site_id	AJ4
Number of Residues	5
Details	binding site for residue CU J 403

Chain	Residue
J	HIS103
J	HIS105
J	HIS157
K	HIS290
K	HOH536

site_id	AJ5
Number of Residues	9
Details	binding site for residue GOL J 404

Chain	Residue
J	GLN257
J	ASN261
J	LYS262
J	ILE263
J	GOL407
J	HOH504
J	HOH539
L	LYS262
L	ASP268

site_id	AJ6
Number of Residues	9
Details	binding site for residue GOL J 405

Chain	Residue
J	GLY152
J	TYR153
J	ARG245
J	SER269
J	HOH510
J	HOH532
J	HOH544
K	GLN257
K	VAL258

site_id	AJ7
Number of Residues	7
Details	binding site for residue GOL J 406

Chain	Residue
J	ASP243
J	GLN257
J	HOH533
K	ASP243
K	GLN257
L	ASP243
L	GLN257

site_id	AJ8
Number of Residues	8
Details	binding site for residue GOL J 407

Chain	Residue
J	LYS262
J	GOL404
K	ASP243
K	LYS262
K	ILE263
K	GOL404
K	GOL406
L	LYS262

site_id	AJ9
Number of Residues	2
Details	binding site for residue SO4 J 408

Chain	Residue
J	ARG58
J	GLY61

site_id	AK1
Number of Residues	4
Details	binding site for residue CU K 401

Chain	Residue
K	HIS232
K	CYS289
K	HIS294
K	MET299

site_id	AK2
Number of Residues	4
Details	binding site for residue CU K 402

Chain	Residue
J	HIS159
K	HIS237
K	HIS288
K	HOH536

site_id	AK3
Number of Residues	5
Details	binding site for residue CU K 403

Chain	Residue
K	HIS103
K	HIS105
K	HIS157
L	HIS290
L	HOH515

site_id	AK4
Number of Residues	9
Details	binding site for residue GOL K 404

Chain	Residue
J	LYS262
J	ASP268
J	GOL407
J	HOH511
K	GLN257
K	ASN261
K	LYS262
K	ILE263
K	HOH503

site_id	AK5
Number of Residues	8
Details	binding site for residue GOL K 405

Chain	Residue
K	GLY152
K	TYR153
K	SER269
K	HOH524
K	HOH537
K	HOH545
L	GLN257
L	VAL258

site_id	AK6
Number of Residues	9
Details	binding site for residue GOL K 406

Chain	Residue
J	GOL407
K	LYS262
K	ASP268
K	HOH513
K	HOH514
L	GLN257
L	ASN261
L	LYS262
L	ILE263

site_id	AK7
Number of Residues	7
Details	binding site for residue GOL K 407

Chain	Residue
K	GLY152
K	TYR153
K	VAL180
K	ARG181
K	ASP185
K	ARG245
K	HOH518

site_id	AK8
Number of Residues	5
Details	binding site for residue CU L 401

Chain	Residue
J	HIS290
J	HOH553
L	HIS103
L	HIS105
L	HIS157

site_id	AK9
Number of Residues	4
Details	binding site for residue CU L 402

Chain	Residue
L	HIS232
L	CYS289
L	HIS294
L	MET299

site_id	AL1
Number of Residues	4
Details	binding site for residue CU L 403

Chain	Residue
K	HIS159
L	HIS237
L	HIS288
L	HOH515

site_id	AL2
Number of Residues	9
Details	binding site for residue GOL L 404

Chain	Residue
J	GLN257
J	VAL258
L	GLY152
L	TYR153
L	TRP154
L	SER269
L	HOH508
L	HOH531
L	HOH542

Functional Information from PROSITE/UniProt

site_id	PS00080
Number of Residues	12
Details	MULTICOPPER_OXIDASE2 Multicopper oxidases signature 2. HCHvqsHsdmGM

Chain	Residue	Details
A	HIS288-MET299

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)