5O4I
Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9687 |
Spacegroup name | P 1 |
Unit cell lengths | 76.933, 94.931, 116.587 |
Unit cell angles | 90.08, 90.20, 91.65 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.17958 |
Rwork | 0.177 |
R-free | 0.22150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o3k |
RMSD bond length | 0.017 |
RMSD bond angle | 1.769 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 283032 |
<I/σ(I)> | 6.81 |
Completeness [%] | 92.6 |
Redundancy | 2.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 23% PEG 4000, 0.05M Tris-HCl |