Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5NC5

Crystal structure of AcrBZ in complex with antibiotic puromycin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0015562	molecular_function	efflux transmembrane transporter activity
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0042802	molecular_function	identical protein binding
A	0042908	biological_process	xenobiotic transport
A	0042910	molecular_function	xenobiotic transmembrane transporter activity
A	0055085	biological_process	transmembrane transport
A	0098567	cellular_component	periplasmic side of plasma membrane
A	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
A	1990281	cellular_component	efflux pump complex
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0015562	molecular_function	efflux transmembrane transporter activity
B	0016020	cellular_component	membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0042802	molecular_function	identical protein binding
B	0042908	biological_process	xenobiotic transport
B	0042910	molecular_function	xenobiotic transmembrane transporter activity
B	0055085	biological_process	transmembrane transport
B	0098567	cellular_component	periplasmic side of plasma membrane
B	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
B	1990281	cellular_component	efflux pump complex
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0015562	molecular_function	efflux transmembrane transporter activity
C	0016020	cellular_component	membrane
C	0022857	molecular_function	transmembrane transporter activity
C	0042802	molecular_function	identical protein binding
C	0042908	biological_process	xenobiotic transport
C	0042910	molecular_function	xenobiotic transmembrane transporter activity
C	0055085	biological_process	transmembrane transport
C	0098567	cellular_component	periplasmic side of plasma membrane
C	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
C	1990281	cellular_component	efflux pump complex
F	0005886	cellular_component	plasma membrane
F	0042910	molecular_function	xenobiotic transmembrane transporter activity
F	0046677	biological_process	response to antibiotic
F	1990961	biological_process	xenobiotic detoxification by transmembrane export across the plasma membrane
G	0005886	cellular_component	plasma membrane
G	0042910	molecular_function	xenobiotic transmembrane transporter activity
G	0046677	biological_process	response to antibiotic
G	1990961	biological_process	xenobiotic detoxification by transmembrane export across the plasma membrane
H	0005886	cellular_component	plasma membrane
H	0042910	molecular_function	xenobiotic transmembrane transporter activity
H	0046677	biological_process	response to antibiotic
H	1990961	biological_process	xenobiotic detoxification by transmembrane export across the plasma membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue LMT A 1101

Chain	Residue
A	ILE27
A	LEU28
A	LEU30
A	VAL32
A	HIS338
A	PHE380
A	D101106

site_id	AC2
Number of Residues	1
Details	binding site for residue LMT A 1102

Chain	Residue
C	ILE18

site_id	AC3
Number of Residues	2
Details	binding site for residue D12 A 1103

Chain	Residue
A	MET20
A	TRP13

site_id	AC4
Number of Residues	2
Details	binding site for residue D10 A 1104

Chain	Residue
A	TRP895
A	D101105

site_id	AC5
Number of Residues	3
Details	binding site for residue D10 A 1105

Chain	Residue
A	PHE885
A	TRP895
A	D101104

site_id	AC6
Number of Residues	2
Details	binding site for residue D10 A 1106

Chain	Residue
A	ILE370
A	LMT1101

site_id	AC7
Number of Residues	3
Details	binding site for residue D10 A 1108

Chain	Residue
A	ALA457
A	PHE458
A	DD91110

site_id	AC8
Number of Residues	2
Details	binding site for residue D10 A 1109

Chain	Residue
A	ALA385
A	DD91114

site_id	AC9
Number of Residues	2
Details	binding site for residue DD9 A 1110

Chain	Residue
A	PHE386
A	D101108

site_id	AD1
Number of Residues	4
Details	binding site for residue LMT A 1111

Chain	Residue
A	LYS29
A	DD91113
B	SER450
B	VAL454

site_id	AD2
Number of Residues	1
Details	binding site for residue D12 A 1112

Chain	Residue
A	ARG8

site_id	AD3
Number of Residues	1
Details	binding site for residue DD9 A 1113

Chain	Residue
A	LMT1111

site_id	AD4
Number of Residues	2
Details	binding site for residue DD9 A 1114

Chain	Residue
A	D101109
B	DD91112

site_id	AD5
Number of Residues	1
Details	binding site for residue D10 B 1101

Chain	Residue
B	TRP895

site_id	AD6
Number of Residues	3
Details	binding site for residue D10 B 1103

Chain	Residue
B	TRP13
B	D101110
C	TRP895

site_id	AD7
Number of Residues	1
Details	binding site for residue D10 B 1105

Chain	Residue
B	PHE885

site_id	AD8
Number of Residues	1
Details	binding site for residue DD9 B 1106

Chain	Residue
C	D101104

site_id	AD9
Number of Residues	2
Details	binding site for residue DD9 B 1107

Chain	Residue
B	LEU25
C	ILE879

site_id	AE1
Number of Residues	1
Details	binding site for residue D10 B 1110

Chain	Residue
B	D101103

site_id	AE2
Number of Residues	3
Details	binding site for residue D10 B 1111

Chain	Residue
B	TYR467
B	TYR877
B	GLN928

site_id	AE3
Number of Residues	2
Details	binding site for residue DD9 B 1112

Chain	Residue
A	DD91114
B	DD91119

site_id	AE4
Number of Residues	2
Details	binding site for residue D10 B 1113

Chain	Residue
B	PHE386
B	SER476

site_id	AE5
Number of Residues	2
Details	binding site for residue D10 B 1114

Chain	Residue
B	PHE4
B	D101122

site_id	AE6
Number of Residues	2
Details	binding site for residue DD9 B 1115

Chain	Residue
B	SER875
B	ILE882

site_id	AE7
Number of Residues	2
Details	binding site for residue D10 B 1116

Chain	Residue
B	SER880
B	LEU881

site_id	AE8
Number of Residues	3
Details	binding site for residue D10 B 1117

Chain	Residue
B	ILE27
B	PHE380
B	ILE390

site_id	AE9
Number of Residues	2
Details	binding site for residue DD9 B 1119

Chain	Residue
B	ALA385
B	DD91112

site_id	AF1
Number of Residues	9
Details	binding site for residue PUY B 1120

Chain	Residue
B	SER134
B	PHE136
B	VAL139
B	PHE178
B	MET573
B	PHE617
B	PHE666
B	LEU668
B	VAL672

site_id	AF2
Number of Residues	4
Details	binding site for residue D12 B 1121

Chain	Residue
B	ARG8
C	MET447
C	ALA890
C	LEU891

site_id	AF3
Number of Residues	1
Details	binding site for residue D10 B 1122

Chain	Residue
B	D101114

site_id	AF4
Number of Residues	2
Details	binding site for residue DD9 C 1101

Chain	Residue
C	PHE458
C	D121108

site_id	AF5
Number of Residues	1
Details	binding site for residue D12 C 1103

Chain	Residue
C	TRP895

site_id	AF6
Number of Residues	2
Details	binding site for residue D10 C 1104

Chain	Residue
B	DD91106
C	PHE458

site_id	AF7
Number of Residues	2
Details	binding site for residue D12 C 1106

Chain	Residue
A	TRP895
C	TRP13

site_id	AF8
Number of Residues	1
Details	binding site for residue D12 C 1107

Chain	Residue
C	PHE458

site_id	AF9
Number of Residues	1
Details	binding site for residue D12 C 1108

Chain	Residue
C	DD91101

site_id	AG1
Number of Residues	1
Details	binding site for residue DD9 C 1109

Chain	Residue
C	DD91110

site_id	AG2
Number of Residues	1
Details	binding site for residue DD9 C 1110

Chain	Residue
C	DD91109

site_id	AG3
Number of Residues	1
Details	binding site for residue D10 C 1112

Chain	Residue
C	GLN439

site_id	AG4
Number of Residues	2
Details	binding site for residue D10 C 1115

Chain	Residue
C	PHE885
C	D101116

site_id	AG5
Number of Residues	2
Details	binding site for residue D10 C 1116

Chain	Residue
C	MET902
C	D101115

site_id	AG6
Number of Residues	1
Details	binding site for residue D10 C 1117

Chain	Residue
C	LEU881

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	TOPO_DOM: Periplasmic => ECO:0000250

Chain	Residue	Details
F	MET1-SER7
B	GLU414-ILE438
B	ALA491-THR538
B	ALA889-PRO898
B	LEU944-LEU972
B	ILE1019-HIS1049
C	MET1-PRO9
C	LEU357-THR365
C	GLU414-ILE438
C	ALA491-THR538
C	ALA889-PRO898
G	MET1-SER7
C	LEU944-LEU972
C	ILE1019-HIS1049
H	MET1-SER7
A	ALA491-THR538
A	ALA889-PRO898
A	LEU944-LEU972
A	ILE1019-HIS1049
B	MET1-PRO9
B	LEU357-THR365

site_id	SWS_FT_FI2
Number of Residues	60
Details	TRANSMEM: Helical => ECO:0000255\|HAMAP-Rule:MF_01484

Chain	Residue	Details
F	LEU8-GLY28
G	LEU8-GLY28
H	LEU8-GLY28

site_id	SWS_FT_FI3
Number of Residues	60
Details	TOPO_DOM: Cytoplasmic => ECO:0000250

Chain	Residue	Details
F	LEU29-HIS49
B	PHE556-ASN871
B	ARG919-ASP924
B	THR993-GLY998
C	LYS29-SER336
C	PHE386-ASN391
C	PHE458-ALA465
C	PHE556-ASN871
C	ARG919-ASP924
C	THR993-GLY998
G	LEU29-HIS49
H	LEU29-HIS49
A	PHE556-ASN871
A	ARG919-ASP924
A	THR993-GLY998
B	LYS29-SER336
B	PHE386-ASN391
B	PHE458-ALA465

site_id	SWS_FT_FI4
Number of Residues	57
Details	TRANSMEM: Helical; Name=2

Chain	Residue	Details
A	ILE337-TYR356
B	ILE337-TYR356
C	ILE337-TYR356

site_id	SWS_FT_FI5
Number of Residues	57
Details	TRANSMEM: Helical; Name=3

Chain	Residue	Details
A	LEU366-ALA385
B	LEU366-ALA385
C	LEU366-ALA385

site_id	SWS_FT_FI6
Number of Residues	63
Details	TRANSMEM: Helical; Name=4

Chain	Residue	Details
A	THR392-VAL413
B	THR392-VAL413
C	THR392-VAL413

site_id	SWS_FT_FI7
Number of Residues	54
Details	TRANSMEM: Helical; Name=5

Chain	Residue	Details
A	GLN439-ALA457
B	GLN439-ALA457
C	GLN439-ALA457

site_id	SWS_FT_FI8
Number of Residues	72
Details	TRANSMEM: Helical; Name=6

Chain	Residue	Details
A	ILE466-PRO490
B	ILE466-PRO490
C	ILE466-PRO490

site_id	SWS_FT_FI9
Number of Residues	48
Details	TRANSMEM: Helical; Name=7

Chain	Residue	Details
A	GLY539-LEU555
B	GLY539-LEU555
C	GLY539-LEU555

site_id	SWS_FT_FI10
Number of Residues	48
Details	TRANSMEM: Helical; Name=8

Chain	Residue	Details
A	GLN872-LEU888
B	GLN872-LEU888
C	GLN872-LEU888

site_id	SWS_FT_FI11
Number of Residues	57
Details	TRANSMEM: Helical; Name=9

Chain	Residue	Details
A	PHE899-PHE918
B	PHE899-PHE918
C	PHE899-PHE918

site_id	SWS_FT_FI12
Number of Residues	54
Details	TRANSMEM: Helical; Name=10

Chain	Residue	Details
A	VAL925-ILE943
B	VAL925-ILE943
C	VAL925-ILE943

site_id	SWS_FT_FI13
Number of Residues	57
Details	TRANSMEM: Helical; Name=11

Chain	Residue	Details
A	ARG973-SER992
B	ARG973-SER992
C	ARG973-SER992

site_id	SWS_FT_FI14
Number of Residues	57
Details	TRANSMEM: Helical; Name=12

Chain	Residue	Details
A	ALA999-ALA1018
B	ALA999-ALA1018
C	ALA999-ALA1018

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)