5N10

Cucurbit[8]uril and 14-3-3 based binary bivalent supramolecular-protein assembly platform

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004860	molecular_function	protein kinase inhibitor activity
A	0004864	molecular_function	protein phosphatase inhibitor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005774	cellular_component	vacuolar membrane
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0007165	biological_process	signal transduction
A	0008104	biological_process	intracellular protein localization
A	0016020	cellular_component	membrane
A	0017053	cellular_component	transcription repressor complex
A	0019899	molecular_function	enzyme binding
A	0019904	molecular_function	protein domain specific binding
A	0032991	cellular_component	protein-containing complex
A	0042308	biological_process	negative regulation of protein import into nucleus
A	0042470	cellular_component	melanosome
A	0042802	molecular_function	identical protein binding
A	0042826	molecular_function	histone deacetylase binding
A	0044877	molecular_function	protein-containing complex binding
A	0045296	molecular_function	cadherin binding
A	0045744	biological_process	negative regulation of G protein-coupled receptor signaling pathway
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050815	molecular_function	phosphoserine residue binding
A	0051219	molecular_function	phosphoprotein binding
A	0070062	cellular_component	extracellular exosome
A	0140311	molecular_function	protein sequestering activity
B	0004860	molecular_function	protein kinase inhibitor activity
B	0004864	molecular_function	protein phosphatase inhibitor activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005774	cellular_component	vacuolar membrane
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0007165	biological_process	signal transduction
B	0008104	biological_process	intracellular protein localization
B	0016020	cellular_component	membrane
B	0017053	cellular_component	transcription repressor complex
B	0019899	molecular_function	enzyme binding
B	0019904	molecular_function	protein domain specific binding
B	0032991	cellular_component	protein-containing complex
B	0042308	biological_process	negative regulation of protein import into nucleus
B	0042470	cellular_component	melanosome
B	0042802	molecular_function	identical protein binding
B	0042826	molecular_function	histone deacetylase binding
B	0044877	molecular_function	protein-containing complex binding
B	0045296	molecular_function	cadherin binding
B	0045744	biological_process	negative regulation of G protein-coupled receptor signaling pathway
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050815	molecular_function	phosphoserine residue binding
B	0051219	molecular_function	phosphoprotein binding
B	0070062	cellular_component	extracellular exosome
B	0140311	molecular_function	protein sequestering activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue GOL A 301

Chain	Residue
A	LYS195
A	PHE198
A	ASP199
A	ARG224
A	HOH467
A	HOH469
A	HOH548

---

site_id	AC2
Number of Residues	23
Details	binding site for residue C8L C 601

Chain	Residue
A	GLU233
B	GLN69
B	THR71
C	PHE581
C	GLY582
C	GLU587
C	GLU589
C	GLY590
C	HOH702
C	HOH704
C	HOH705
C	HOH713
C	HOH715
C	HOH723
C	HOH725
C	HOH727
C	HOH730
D	PHE581
D	GLY582
D	GLY583
D	HOH601
A	LEU229
A	SER232

---

Functional Information from PROSITE/UniProt

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
A	ARG43-VAL53
B	ARG43-VAL53

---

site_id	PS00797
Number of Residues	20
Details	1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTS

Chain	Residue	Details
A	TYR213-SER232
B	TYR213-SER232

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Site: {"description":"Interaction with phosphoserine on interacting protein","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P27348","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P27348","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9CQV8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"Q9CQV8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68251","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P27348","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---