5MIV

G307E variant of murine Apoptosis Inducing Factor in complex with NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0046983	molecular_function	protein dimerization activity
C	0016491	molecular_function	oxidoreductase activity
C	0046983	molecular_function	protein dimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue NAD A 701

Chain	Residue
A	ARG264
A	GLY398
A	GLU452
A	HIS453
A	TRP482
A	FAD702
A	HOH801
A	HOH805
A	HOH829
A	GLU307
A	GLY308
A	PHE309
A	LEU310
A	GLU313
A	PRO334
A	ALA396
A	VAL397

---

site_id	AC2
Number of Residues	30
Details	binding site for residue FAD A 702

Chain	Residue
A	GLY137
A	GLY138
A	GLY139
A	THR140
A	ALA141
A	VAL161
A	GLU163
A	ASP164
A	ARG171
A	PRO172
A	SER175
A	VAL232
A	ALA258
A	THR259
A	GLY260
A	ARG284
A	GLU313
A	ASN402
A	GLY436
A	ASP437
A	GLU452
A	HIS453
A	HIS454
A	ALA457
A	PHE481
A	TRP482
A	NAD701
A	HOH806
A	HOH810
A	HOH814

---

site_id	AC3
Number of Residues	17
Details	binding site for residue NAD C 701

Chain	Residue
C	ARG264
C	GLU307
C	PHE309
C	LEU310
C	GLU313
C	LYS341
C	ALA396
C	VAL397
C	GLY398
C	GLU452
C	HIS453
C	TRP482
C	FAD702
C	HOH805
C	HOH821
C	HOH823
C	HOH829

---

site_id	AC4
Number of Residues	33
Details	binding site for residue FAD C 702

Chain	Residue
C	GLY137
C	GLY138
C	GLY139
C	THR140
C	ALA141
C	VAL161
C	GLU163
C	ASP164
C	ARG171
C	PRO172
C	SER175
C	VAL232
C	ALA258
C	THR259
C	GLY260
C	ARG284
C	GLU313
C	ASN402
C	GLY436
C	ASP437
C	GLU452
C	HIS453
C	HIS454
C	ALA457
C	PHE481
C	TRP482
C	NAD701
C	HOH801
C	HOH805
C	HOH807
C	HOH827
C	HOH830
C	HOH836

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	698
Details	Region: {"description":"FAD-dependent oxidoreductase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Motif: {"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11967568","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19447115","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3GD3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3GD4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11967568","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19447115","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3GD4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11967568","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19447115","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3GD3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"O95831","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19447115","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3GD4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19447115","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3GD3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3GD4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"O95831","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"23576753","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"O95831","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---