Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5ME0

Structure of the 30S Pre-Initiation Complex 1 (30S IC-1) Stalled by GE81112

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000028	biological_process	ribosomal small subunit assembly
B	0002181	biological_process	cytoplasmic translation
B	0003735	molecular_function	structural constituent of ribosome
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005840	cellular_component	ribosome
B	0006412	biological_process	translation
B	0008270	molecular_function	zinc ion binding
B	0015935	cellular_component	small ribosomal subunit
B	0022627	cellular_component	cytosolic small ribosomal subunit
B	1990904	cellular_component	ribonucleoprotein complex
C	0000028	biological_process	ribosomal small subunit assembly
C	0002181	biological_process	cytoplasmic translation
C	0003676	molecular_function	nucleic acid binding
C	0003723	molecular_function	RNA binding
C	0003729	molecular_function	mRNA binding
C	0003735	molecular_function	structural constituent of ribosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005840	cellular_component	ribosome
C	0006412	biological_process	translation
C	0019843	molecular_function	rRNA binding
C	0022627	cellular_component	cytosolic small ribosomal subunit
C	1990904	cellular_component	ribonucleoprotein complex
D	0000028	biological_process	ribosomal small subunit assembly
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0002181	biological_process	cytoplasmic translation
D	0003723	molecular_function	RNA binding
D	0003735	molecular_function	structural constituent of ribosome
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005840	cellular_component	ribosome
D	0006353	biological_process	DNA-templated transcription termination
D	0006412	biological_process	translation
D	0006417	biological_process	regulation of translation
D	0015935	cellular_component	small ribosomal subunit
D	0019843	molecular_function	rRNA binding
D	0022627	cellular_component	cytosolic small ribosomal subunit
D	0031564	biological_process	transcription antitermination
D	0042254	biological_process	ribosome biogenesis
D	0042274	biological_process	ribosomal small subunit biogenesis
D	0045947	biological_process	negative regulation of translational initiation
D	0046677	biological_process	response to antibiotic
D	0048027	molecular_function	mRNA 5'-UTR binding
D	1990145	biological_process	maintenance of translational fidelity
D	1990904	cellular_component	ribonucleoprotein complex
E	0000028	biological_process	ribosomal small subunit assembly
E	0002181	biological_process	cytoplasmic translation
E	0003723	molecular_function	RNA binding
E	0003735	molecular_function	structural constituent of ribosome
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005840	cellular_component	ribosome
E	0006412	biological_process	translation
E	0015935	cellular_component	small ribosomal subunit
E	0019843	molecular_function	rRNA binding
E	0022627	cellular_component	cytosolic small ribosomal subunit
E	0043229	cellular_component	intracellular organelle
E	0046677	biological_process	response to antibiotic
E	1990145	biological_process	maintenance of translational fidelity
E	1990904	cellular_component	ribonucleoprotein complex
F	0002181	biological_process	cytoplasmic translation
F	0003723	molecular_function	RNA binding
F	0003735	molecular_function	structural constituent of ribosome
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005840	cellular_component	ribosome
F	0006412	biological_process	translation
F	0019843	molecular_function	rRNA binding
F	0022627	cellular_component	cytosolic small ribosomal subunit
F	0043229	cellular_component	intracellular organelle
F	0048027	molecular_function	mRNA 5'-UTR binding
F	0070181	molecular_function	small ribosomal subunit rRNA binding
F	1990904	cellular_component	ribonucleoprotein complex
G	0003723	molecular_function	RNA binding
G	0003735	molecular_function	structural constituent of ribosome
G	0006412	biological_process	translation
G	0015935	cellular_component	small ribosomal subunit
H	0000028	biological_process	ribosomal small subunit assembly
H	0002181	biological_process	cytoplasmic translation
H	0003723	molecular_function	RNA binding
H	0003735	molecular_function	structural constituent of ribosome
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005840	cellular_component	ribosome
H	0006412	biological_process	translation
H	0006417	biological_process	regulation of translation
H	0019843	molecular_function	rRNA binding
H	0022627	cellular_component	cytosolic small ribosomal subunit
H	0043229	cellular_component	intracellular organelle
H	0043488	biological_process	regulation of mRNA stability
H	1990904	cellular_component	ribonucleoprotein complex
I	0000049	molecular_function	tRNA binding
I	0002181	biological_process	cytoplasmic translation
I	0003723	molecular_function	RNA binding
I	0003735	molecular_function	structural constituent of ribosome
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005840	cellular_component	ribosome
I	0006412	biological_process	translation
I	0022627	cellular_component	cytosolic small ribosomal subunit
I	0043229	cellular_component	intracellular organelle
I	1990904	cellular_component	ribonucleoprotein complex
J	0000049	molecular_function	tRNA binding
J	0001072	molecular_function	transcription antitermination factor activity, RNA binding
J	0002181	biological_process	cytoplasmic translation
J	0003723	molecular_function	RNA binding
J	0003735	molecular_function	structural constituent of ribosome
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005840	cellular_component	ribosome
J	0006412	biological_process	translation
J	0008023	cellular_component	transcription elongation factor complex
J	0015935	cellular_component	small ribosomal subunit
J	0022627	cellular_component	cytosolic small ribosomal subunit
J	0031564	biological_process	transcription antitermination
J	0032784	biological_process	regulation of DNA-templated transcription elongation
J	0042254	biological_process	ribosome biogenesis
J	1990904	cellular_component	ribonucleoprotein complex
K	0002181	biological_process	cytoplasmic translation
K	0003723	molecular_function	RNA binding
K	0003735	molecular_function	structural constituent of ribosome
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005840	cellular_component	ribosome
K	0006412	biological_process	translation
K	0019843	molecular_function	rRNA binding
K	0022627	cellular_component	cytosolic small ribosomal subunit
K	0070181	molecular_function	small ribosomal subunit rRNA binding
K	1990904	cellular_component	ribonucleoprotein complex
L	0000049	molecular_function	tRNA binding
L	0000372	biological_process	Group I intron splicing
L	0002181	biological_process	cytoplasmic translation
L	0003723	molecular_function	RNA binding
L	0003735	molecular_function	structural constituent of ribosome
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005840	cellular_component	ribosome
L	0006412	biological_process	translation
L	0015935	cellular_component	small ribosomal subunit
L	0019843	molecular_function	rRNA binding
L	0022627	cellular_component	cytosolic small ribosomal subunit
L	0033120	biological_process	positive regulation of RNA splicing
L	0034336	molecular_function	misfolded RNA binding
L	0034337	biological_process	RNA folding
L	0046677	biological_process	response to antibiotic
L	1990145	biological_process	maintenance of translational fidelity
L	1990904	cellular_component	ribonucleoprotein complex
M	0000049	molecular_function	tRNA binding
M	0002181	biological_process	cytoplasmic translation
M	0003676	molecular_function	nucleic acid binding
M	0003723	molecular_function	RNA binding
M	0003735	molecular_function	structural constituent of ribosome
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005840	cellular_component	ribosome
M	0006412	biological_process	translation
M	0015935	cellular_component	small ribosomal subunit
M	0019843	molecular_function	rRNA binding
M	0022627	cellular_component	cytosolic small ribosomal subunit
M	1990904	cellular_component	ribonucleoprotein complex
N	0000028	biological_process	ribosomal small subunit assembly
N	0000049	molecular_function	tRNA binding
N	0002181	biological_process	cytoplasmic translation
N	0003735	molecular_function	structural constituent of ribosome
N	0005737	cellular_component	cytoplasm
N	0005840	cellular_component	ribosome
N	0006412	biological_process	translation
N	0015935	cellular_component	small ribosomal subunit
N	0019843	molecular_function	rRNA binding
N	0022627	cellular_component	cytosolic small ribosomal subunit
N	0043229	cellular_component	intracellular organelle
N	1990904	cellular_component	ribonucleoprotein complex
O	0000028	biological_process	ribosomal small subunit assembly
O	0002181	biological_process	cytoplasmic translation
O	0003723	molecular_function	RNA binding
O	0003735	molecular_function	structural constituent of ribosome
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005840	cellular_component	ribosome
O	0006412	biological_process	translation
O	0006417	biological_process	regulation of translation
O	0019843	molecular_function	rRNA binding
O	0022627	cellular_component	cytosolic small ribosomal subunit
O	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
O	0043229	cellular_component	intracellular organelle
O	0070181	molecular_function	small ribosomal subunit rRNA binding
O	1903231	molecular_function	mRNA base-pairing translational repressor activity
O	1990904	cellular_component	ribonucleoprotein complex
P	0003735	molecular_function	structural constituent of ribosome
P	0005840	cellular_component	ribosome
P	0006412	biological_process	translation
Q	0000028	biological_process	ribosomal small subunit assembly
Q	0002181	biological_process	cytoplasmic translation
Q	0003723	molecular_function	RNA binding
Q	0003735	molecular_function	structural constituent of ribosome
Q	0005737	cellular_component	cytoplasm
Q	0005840	cellular_component	ribosome
Q	0006412	biological_process	translation
Q	0008270	molecular_function	zinc ion binding
Q	0019843	molecular_function	rRNA binding
Q	0022627	cellular_component	cytosolic small ribosomal subunit
Q	0046677	biological_process	response to antibiotic
Q	0060090	molecular_function	molecular adaptor activity
Q	0070181	molecular_function	small ribosomal subunit rRNA binding
Q	1990904	cellular_component	ribonucleoprotein complex
R	0002181	biological_process	cytoplasmic translation
R	0003723	molecular_function	RNA binding
R	0003735	molecular_function	structural constituent of ribosome
R	0005515	molecular_function	protein binding
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005840	cellular_component	ribosome
R	0006412	biological_process	translation
R	0019843	molecular_function	rRNA binding
R	0022627	cellular_component	cytosolic small ribosomal subunit
R	0048027	molecular_function	mRNA 5'-UTR binding
R	0070181	molecular_function	small ribosomal subunit rRNA binding
R	1990904	cellular_component	ribonucleoprotein complex
S	0000028	biological_process	ribosomal small subunit assembly
S	0000049	molecular_function	tRNA binding
S	0002181	biological_process	cytoplasmic translation
S	0003723	molecular_function	RNA binding
S	0003735	molecular_function	structural constituent of ribosome
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005840	cellular_component	ribosome
S	0006412	biological_process	translation
S	0015935	cellular_component	small ribosomal subunit
S	0019843	molecular_function	rRNA binding
S	0022627	cellular_component	cytosolic small ribosomal subunit
S	0043229	cellular_component	intracellular organelle
S	1990904	cellular_component	ribonucleoprotein complex
T	0000028	biological_process	ribosomal small subunit assembly
T	0002181	biological_process	cytoplasmic translation
T	0003723	molecular_function	RNA binding
T	0003735	molecular_function	structural constituent of ribosome
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005840	cellular_component	ribosome
T	0006412	biological_process	translation
T	0008073	molecular_function	ornithine decarboxylase inhibitor activity
T	0015935	cellular_component	small ribosomal subunit
T	0019843	molecular_function	rRNA binding
T	0022627	cellular_component	cytosolic small ribosomal subunit
T	0070181	molecular_function	small ribosomal subunit rRNA binding
T	1990904	cellular_component	ribonucleoprotein complex
U	0000028	biological_process	ribosomal small subunit assembly
U	0002181	biological_process	cytoplasmic translation
U	0003723	molecular_function	RNA binding
U	0003735	molecular_function	structural constituent of ribosome
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005840	cellular_component	ribosome
U	0006412	biological_process	translation
U	0019843	molecular_function	rRNA binding
U	0022627	cellular_component	cytosolic small ribosomal subunit
U	1990904	cellular_component	ribonucleoprotein complex
V	0003723	molecular_function	RNA binding
V	0003743	molecular_function	translation initiation factor activity
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0006412	biological_process	translation
V	0006413	biological_process	translational initiation
V	0019843	molecular_function	rRNA binding
V	0043022	molecular_function	ribosome binding
W	0000166	molecular_function	nucleotide binding
W	0003743	molecular_function	translation initiation factor activity
W	0003924	molecular_function	GTPase activity
W	0005515	molecular_function	protein binding
W	0005525	molecular_function	GTP binding
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0006412	biological_process	translation
W	0006413	biological_process	translational initiation
W	0009409	biological_process	response to cold
W	0016020	cellular_component	membrane
W	0043024	molecular_function	ribosomal small subunit binding
W	0061077	biological_process	chaperone-mediated protein folding
W	0097216	molecular_function	guanosine tetraphosphate binding
Y	0003743	molecular_function	translation initiation factor activity
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0006412	biological_process	translation
Y	0006413	biological_process	translational initiation
Y	0016020	cellular_component	membrane
Y	0032790	biological_process	ribosome disassembly
Y	0043022	molecular_function	ribosome binding
Z	0003743	molecular_function	translation initiation factor activity
Z	0006413	biological_process	translational initiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue FME X 101

Chain	Residue
W	ARG801
W	GLY862
X	A76
W	PHE804
W	GLY814
W	CYS815
W	ARG846
W	ARG847
W	PHE848
W	GLU860
W	CYS861

Functional Information from PROSITE/UniProt

site_id	PS00362
Number of Residues	31
Details	RIBOSOMAL_S15 Ribosomal protein S15 signature. LqgHFaehKkDhhSrrgLlrMvsqrrkLldY

Chain	Residue	Details
O	LEU39-TYR69

site_id	PS01176
Number of Residues	23
Details	IF2 Initiation factor 2 signature. GeLesLRRfKddvneVrnGmECG

Chain	Residue	Details
W	GLY840-GLY862

site_id	PS00548
Number of Residues	35
Details	RIBOSOMAL_S3 Ribosomal protein S3 signature. ARtewyreGrVplHt..LradIDyntseahTtyGviG

Chain	Residue	Details
C	ALA163-GLY197

site_id	PS00057
Number of Residues	24
Details	RIBOSOMAL_S18 Ribosomal protein S18 signature. IDYkd.IatLknYITEs.GKIvpsRI

Chain	Residue	Details
R	ILE21-ILE44

site_id	PS00056
Number of Residues	13
Details	RIBOSOMAL_S17 Ribosomal protein S17 signature. GDvVeIrEcRPLS

Chain	Residue	Details
Q	GLY56-SER68

site_id	PS00055
Number of Residues	8
Details	RIBOSOMAL_S12 Ribosomal protein S12 signature. KkPNSAlR

Chain	Residue	Details
L	LYS43-ARG50

site_id	PS00054
Number of Residues	23
Details	RIBOSOMAL_S11 Ribosomal protein S11 signature. IrALnaA.GFrItnitDvTPiPHN

Chain	Residue	Details
K	ILE97-ASN119

site_id	PS00361
Number of Residues	16
Details	RIBOSOMAL_S10 Ribosomal protein S10 signature. AkrtGAqvrGPIpLPT

Chain	Residue	Details
J	ALA29-THR44

site_id	PS01181
Number of Residues	13
Details	RIBOSOMAL_S21 Ribosomal protein S21 signature. DvALRRFKRsceK

Chain	Residue	Details
U	ASP13-LYS25

site_id	PS00360
Number of Residues	19
Details	RIBOSOMAL_S9 Ribosomal protein S9 signature. GGGisGQagAirhGiTRAL

Chain	Residue	Details
I	GLY69-LEU87

site_id	PS00585
Number of Residues	33
Details	RIBOSOMAL_S5 Ribosomal protein S5 signature. GRifsFtAltVVGDgn.GrVGfGygkar.EVpa.AI

Chain	Residue	Details
E	GLY28-ILE60

site_id	PS00732
Number of Residues	10
Details	RIBOSOMAL_S16 Ribosomal protein S16 signature. VtIRLARHGA

Chain	Residue	Details
P	VAL2-ALA11

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. PdhkhavialTsiYGVGKTRSkAILaAAG

Chain	Residue	Details
M	PRO10-GLY38

site_id	PS00646
Number of Residues	14
Details	RIBOSOMAL_S13_1 Ribosomal protein S13 signature. RGlRHrrGlpVRGQ

Chain	Residue	Details
M	ARG87-GLN100

site_id	PS00527
Number of Residues	23
Details	RIBOSOMAL_S14 Ribosomal protein S14 signature. R.CrqtgrphgflrkFGLSRiKVR

Chain	Residue	Details
N	ARG63-ARG85

site_id	PS00962
Number of Residues	12
Details	RIBOSOMAL_S2_1 Ribosomal protein S2 signature 1. MrDMLKAGVHFG

Chain	Residue	Details
B	MET6-GLY17

site_id	PS00963
Number of Residues	25
Details	RIBOSOMAL_S2_2 Ribosomal protein S2 signature 2. PdaLFViDadhEhiaIkEannlGIP

Chain	Residue	Details
B	PRO158-PRO182

site_id	PS00323
Number of Residues	25
Details	RIBOSOMAL_S19 Ribosomal protein S19 signature. NGRqhvpvfVtdemVGhkLGEFapT

Chain	Residue	Details
S	ASN53-THR77

site_id	PS00632
Number of Residues	25
Details	RIBOSOMAL_S4 Ribosomal protein S4 signature. LEgRLdnvVYRmgfGaTraeARqLV

Chain	Residue	Details
D	LEU94-VAL118

site_id	PS00053
Number of Residues	18
Details	RIBOSOMAL_S8 Ribosomal protein S8 signature. GiaVVSTSkGVMtdraAR

Chain	Residue	Details
H	GLY100-ARG117

site_id	PS01048
Number of Residues	10
Details	RIBOSOMAL_S6 Ribosomal protein S6 signature. GrRQLAYpIN

Chain	Residue	Details
F	GLY43-ASN52

site_id	PS00052
Number of Residues	27
Details	RIBOSOMAL_S7 Ribosomal protein S7 signature. SeLlakFVnilmvdGKKstaesIVysA

Chain	Residue	Details
G	SER20-ALA46

site_id	PS00938
Number of Residues	14
Details	IF3 Initiation factor 3 signature. RIMDYGKFrFEqqK

Chain	Residue	Details
Y	ARG58-LYS71
Z	ARG66-LYS79

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Important for 30S binding => ECO:0000305\|PubMed:1457399

Chain	Residue	Details
Z	TYR107
Z	LYS110
W	ASN498

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:18723842

Chain	Residue	Details
W	LYS808

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)