5ME0
Structure of the 30S Pre-Initiation Complex 1 (30S IC-1) Stalled by GE81112
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | JEOL 2200FSC |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Energy Filter | In-column Omega Filter |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 13.5 |
Number of Particle | 174142 |
Software | RELION,Coot,DigitalMicrograph,CTFFIND,Situs |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |