Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LW1

Crystal structure of DARPin-DARPin rigid fusion, variant DD_232_11_D12 in complex JNK1a1 and JIP1 peptide

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0004672	molecular_function	protein kinase activity
B	0004707	molecular_function	MAP kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation
E	0004672	molecular_function	protein kinase activity
E	0004707	molecular_function	MAP kinase activity
E	0005524	molecular_function	ATP binding
E	0006468	biological_process	protein phosphorylation
H	0004672	molecular_function	protein kinase activity
H	0004707	molecular_function	MAP kinase activity
H	0005524	molecular_function	ATP binding
H	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue SO4 A 401

Chain	Residue
A	GLY215
A	LEU217
A	GLY218

site_id	AC2
Number of Residues	4
Details	binding site for residue SO4 A 402

Chain	Residue
A	PHE89
A	HIS123
A	HIS125
B	TYR230

site_id	AC3
Number of Residues	9
Details	binding site for residue ADN B 401

Chain	Residue
B	ALA53
B	MET108
B	GLU109
B	MET111
B	ASN114
B	SER155
B	LEU168
B	GLY33
B	VAL40

site_id	AC4
Number of Residues	2
Details	binding site for residue SO4 B 402

Chain	Residue
B	LYS250
B	ARG257

site_id	AC5
Number of Residues	1
Details	binding site for residue SO4 B 403

Chain	Residue
B	ARG127

site_id	AC6
Number of Residues	3
Details	binding site for residue SO4 B 404

Chain	Residue
B	ARG189
B	ARG192
B	TYR230

site_id	AC7
Number of Residues	7
Details	binding site for residue SO4 B 405

Chain	Residue
B	MET181
B	THR183
B	VAL186
B	VAL187
B	THR188
D	TRP213
D	TYR246

site_id	AC8
Number of Residues	2
Details	binding site for residue SO4 B 406

Chain	Residue
B	THR348
B	ILE349

site_id	AC9
Number of Residues	3
Details	binding site for residue SO4 D 401

Chain	Residue
D	HIS216
D	LEU217
D	GLY218

site_id	AD1
Number of Residues	4
Details	binding site for residue SO4 D 402

Chain	Residue
D	GLY281
D	HIS282
D	LEU283
D	GLU284

site_id	AD2
Number of Residues	2
Details	binding site for residue SO4 D 403

Chain	Residue
D	ARG184
D	GLU221

site_id	AD3
Number of Residues	2
Details	binding site for residue SO4 D 404

Chain	Residue
D	VAL263
D	ASN264

site_id	AD4
Number of Residues	9
Details	binding site for residue ADN E 401

Chain	Residue
E	GLY33
E	VAL40
E	ALA53
E	ILE86
E	MET108
E	GLU109
E	MET111
E	ASN114
E	SER155

site_id	AD5
Number of Residues	3
Details	binding site for residue SO4 E 402

Chain	Residue
E	ARG189
E	ARG192
E	TYR230

site_id	AD6
Number of Residues	1
Details	binding site for residue SO4 E 403

Chain	Residue
E	ARG257

site_id	AD7
Number of Residues	6
Details	binding site for residue SO4 E 404

Chain	Residue
E	THR183
E	VAL186
E	VAL187
E	THR188
G	TRP213
G	TYR246

site_id	AD8
Number of Residues	1
Details	binding site for residue SO4 E 405

Chain	Residue
E	SER12

site_id	AD9
Number of Residues	3
Details	binding site for residue SO4 G 401

Chain	Residue
G	GLY215
G	LEU217
G	GLY218

site_id	AE1
Number of Residues	3
Details	binding site for residue SO4 G 402

Chain	Residue
G	LYS16
G	ASP44
G	GLN45

site_id	AE2
Number of Residues	4
Details	binding site for residue SO4 G 403

Chain	Residue
G	GLY281
G	HIS282
G	LEU283
G	GLU284

site_id	AE3
Number of Residues	2
Details	binding site for residue SO4 G 404

Chain	Residue
G	VAL263
G	ASN264

site_id	AE4
Number of Residues	9
Details	binding site for residue ADN H 401

Chain	Residue
H	SER34
H	VAL40
H	ALA53
H	MET108
H	GLU109
H	MET111
H	ASN114
H	SER155
H	LEU168

site_id	AE5
Number of Residues	3
Details	binding site for residue SO4 H 402

Chain	Residue
H	ARG189
H	ARG192
H	TYR230

site_id	AE6
Number of Residues	3
Details	binding site for residue SO4 H 403

Chain	Residue
G	ARG111
H	LYS250
H	ARG257

site_id	AE7
Number of Residues	2
Details	binding site for residue SO4 H 404

Chain	Residue
H	LYS290
H	GLN293

site_id	AE8
Number of Residues	2
Details	binding site for residue SO4 H 405

Chain	Residue
H	TYR11
H	SER12

site_id	AE9
Number of Residues	2
Details	binding site for residue SO4 I 201

Chain	Residue
A	ASN69
I	LEU160

site_id	AF1
Number of Residues	9
Details	binding site for Pepstatin chain L

Chain	Residue
H	THR178
H	GLY199
H	GLY201
H	TYR230
H	ILE231
G	LEU114
G	ILE122
G	HIS123
G	ASN156

Functional Information from PROSITE/UniProt

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDLKpsNIVV

Chain	Residue	Details
B	ILE147-VAL159

site_id	PS01351
Number of Residues	103
Details	MAPK MAP kinase signature. FqnqthakrayRElvlmkcvnhkniigllnvftpqksleefqdvyivmelmdanlcqviqmeldhermsyllyqmlcgikhlhsagiih..........RDlKpsnivvksdC

Chain	Residue	Details
B	PHE61-CYS163

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	885
Details	Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	6
Details	Motif: {"description":"TXY"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	27
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	3
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"UniProtKB","id":"P49185","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	3
Details	Modified residue: {"description":"Phosphothreonine; by MAP2K7","evidences":[{"source":"PubMed","id":"11062067","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	3
Details	Modified residue: {"description":"Phosphotyrosine; by MAP2K4","evidences":[{"source":"PubMed","id":"11062067","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)